+Open data
-Basic information
Entry | Database: PDB / ID: 6hif | ||||||
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Title | Kuenenia stuttgartiensis hydrazine dehydrogenase complex | ||||||
Components |
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Keywords | OXIDOREDUCTASE / anammox / hydrazine / dehydrogenase / P460 / redox | ||||||
Function / homology | Function and homology information hydrazine dehydrogenase / hydroxylamine oxidoreductase activity / anammoxosome / membrane => GO:0016020 / metal ion binding Similarity search - Function | ||||||
Biological species | Kuenenia stuttgartiensis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Akram, M. / Dietl, A. / Mersdorf, U. / Prinz, S. / Maalcke, W. / Keltjens, J. / Ferousi, C. / de Almeida, N.M. / Reimann, J. / Kartal, B. ...Akram, M. / Dietl, A. / Mersdorf, U. / Prinz, S. / Maalcke, W. / Keltjens, J. / Ferousi, C. / de Almeida, N.M. / Reimann, J. / Kartal, B. / Jetten, M.S.M. / Parey, K. / Barends, T.R.M. | ||||||
Citation | Journal: Sci Adv / Year: 2019 Title: A 192-heme electron transfer network in the hydrazine dehydrogenase complex. Authors: M Akram / A Dietl / U Mersdorf / S Prinz / W Maalcke / J Keltjens / C Ferousi / N M de Almeida / J Reimann / B Kartal / M S M Jetten / K Parey / T R M Barends / Abstract: Anaerobic ammonium oxidation (anammox) is a major process in the biogeochemical nitrogen cycle in which nitrite and ammonium are converted to dinitrogen gas and water through the highly reactive ...Anaerobic ammonium oxidation (anammox) is a major process in the biogeochemical nitrogen cycle in which nitrite and ammonium are converted to dinitrogen gas and water through the highly reactive intermediate hydrazine. So far, it is unknown how anammox organisms convert the toxic hydrazine into nitrogen and harvest the extremely low potential electrons (-750 mV) released in this process. We report the crystal structure and cryo electron microscopy structures of the responsible enzyme, hydrazine dehydrogenase, which is a 1.7 MDa multiprotein complex containing an extended electron transfer network of 192 heme groups spanning the entire complex. This unique molecular arrangement suggests a way in which the protein stores and releases the electrons obtained from hydrazine conversion, the final step in the globally important anammox process. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6hif.cif.gz | 2.8 MB | Display | PDBx/mmCIF format |
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PDB format | pdb6hif.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 6hif.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hi/6hif ftp://data.pdbj.org/pub/pdb/validation_reports/hi/6hif | HTTPS FTP |
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-Related structure data
Related structure data | 0205C 0206C 0207C 4n4jS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
NCS oper:
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-Components
-Protein , 2 types, 36 molecules GABCDEFHIJKLMNOPQRSTUVWXYZabcd...
#1: Protein | Mass: 65673.766 Da / Num. of mol.: 24 / Source method: isolated from a natural source / Source: (natural) Kuenenia stuttgartiensis (bacteria) / References: UniProt: Q1PW30, hydrazine dehydrogenase #2: Protein | Mass: 11872.622 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Kuenenia stuttgartiensis (bacteria) / References: UniProt: Q1PXB2, UniProt: A0A2C9CI58*PLUS |
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-Non-polymers , 5 types, 1221 molecules
#3: Chemical | ChemComp-HEC / #4: Chemical | ChemComp-SO4 / #5: Chemical | ChemComp-GOL / #6: Chemical | ChemComp-K / #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 59.07 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 1+1 ul hanging drops against a reservoir solution containing 8-9% (w/v) polyethylene glycol 4000, 0.25 M (NH4)2SO4. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.95376 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 10, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95376 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→185 Å / Num. obs: 452098 / % possible obs: 96.6 % / Redundancy: 2 % / CC1/2: 0.989 / Rmerge(I) obs: 0.111 / Rrim(I) all: 0.147 / Net I/σ(I): 6.67 |
Reflection shell | Resolution: 2.8→2.9 Å / Rmerge(I) obs: 0.745 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 49339 / CC1/2: 0.457 / Rrim(I) all: 0.994 / % possible all: 98.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4N4J Resolution: 2.8→93.71 Å / Cor.coef. Fo:Fc: 0.923 / Cor.coef. Fo:Fc free: 0.915 / SU B: 19.245 / SU ML: 0.338 / Cross valid method: THROUGHOUT / ESU R Free: 0.352 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 63.322 Å2
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Refinement step | Cycle: 1 / Resolution: 2.8→93.71 Å
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Refine LS restraints |
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