+Open data
-Basic information
Entry | Database: PDB / ID: 6a05 | ||||||
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Title | Structure of pSTING complex | ||||||
Components | Stimulator of interferon genes protein | ||||||
Keywords | SIGNALING PROTEIN / pSTING | ||||||
Function / homology | Function and homology information proton channel activity / 2',3'-cyclic GMP-AMP binding / cyclic-di-GMP binding / cGAS/STING signaling pathway / reticulophagy / autophagosome membrane / positive regulation of macroautophagy / autophagosome assembly / autophagosome / positive regulation of type I interferon production ...proton channel activity / 2',3'-cyclic GMP-AMP binding / cyclic-di-GMP binding / cGAS/STING signaling pathway / reticulophagy / autophagosome membrane / positive regulation of macroautophagy / autophagosome assembly / autophagosome / positive regulation of type I interferon production / signaling adaptor activity / positive regulation of interferon-beta production / activation of innate immune response / endoplasmic reticulum-Golgi intermediate compartment membrane / cytoplasmic vesicle / defense response to virus / mitochondrial outer membrane / Golgi membrane / innate immune response / endoplasmic reticulum membrane / perinuclear region of cytoplasm / protein homodimerization activity / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.2 Å | ||||||
Authors | Yuan, Z.L. / Shang, G.J. / Cong, X.Y. / Gu, L.C. | ||||||
Funding support | 1items
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Citation | Journal: J.Biol.Chem. / Year: 2019 Title: Crystal structures of porcine STINGCBD-CDN complexes reveal the mechanism of ligand recognition and discrimination of STING proteins. Authors: Cong, X. / Yuan, Z. / Du, Y. / Wu, B. / Lu, D. / Wu, X. / Zhang, Y. / Li, F. / Wei, B. / Li, J. / Wu, J. / Xu, S. / Wang, J. / Qi, J. / Shang, G. / Gu, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6a05.cif.gz | 88.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6a05.ent.gz | 66.1 KB | Display | PDB format |
PDBx/mmJSON format | 6a05.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a0/6a05 ftp://data.pdbj.org/pub/pdb/validation_reports/a0/6a05 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 22833.762 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / Gene: TMEM173, STING / Production host: Escherichia coli (E. coli) / References: UniProt: B8XX90 #2: Chemical | #3: Chemical | ChemComp-1YD / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.77 Å3/Da / Density % sol: 30.45 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 0.1 M NaOAC (pH = 4.6) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.98 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 6, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 16950 / % possible obs: 100 % / Redundancy: 6.9 % / Net I/σ(I): 30.52 |
Reflection shell | Resolution: 2.2→2.28 Å |
-Processing
Software |
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Refinement | Resolution: 2.2→44.376 Å / SU ML: 0.32 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 34.25 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→44.376 Å
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Refine LS restraints |
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LS refinement shell |
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