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Yorodumi- PDB-2v9a: Structure of Citrate-free Periplasmic Domain of Sensor Histidine ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2v9a | ||||||
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Title | Structure of Citrate-free Periplasmic Domain of Sensor Histidine Kinase CitA | ||||||
Components | SENSOR KINASE CITA | ||||||
Keywords | TRANSFERASE / SIGNAL TRANSDUCTION / SENSOR HISTIDINE KINASE / CITA / KINASE / MEMBRANE / SENSOR DOMAIN / TRANSMEMBRANE / INNER MEMBRANE / PHOSPHORYLATION / TWO-COMPONENT REGULATORY SYSTEM | ||||||
Function / homology | Function and homology information histidine kinase / phosphorelay sensor kinase activity / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | KLEBSIELLA PNEUMONIAE (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Sevvana, M. / Vijayan, V. / Zweckstetter, M. / Reinelt, S. / Madden, D.R. / Sheldrick, G.M. / Bott, M. / Griesinger, C. / Becker, S. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2008 Title: A Ligand-Induced Switch in the Periplasmic Domain of Sensor Histidine Kinase Cita. Authors: Sevvana, M. / Vijayan, V. / Zweckstetter, M. / Reinelt, S. / Madden, D.R. / Herbst-Irmer, R. / Sheldrick, G.M. / Bott, M. / Griesinger, C. / Becker, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2v9a.cif.gz | 56.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2v9a.ent.gz | 40.2 KB | Display | PDB format |
PDBx/mmJSON format | 2v9a.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v9/2v9a ftp://data.pdbj.org/pub/pdb/validation_reports/v9/2v9a | HTTPS FTP |
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-Related structure data
Related structure data | 2j80SC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 14499.438 Da / Num. of mol.: 2 Fragment: PERIPLASMIC LIGAND BINDING DOMAIN, RESIDUES 45-176 Source method: isolated from a genetically manipulated source Source: (gene. exp.) KLEBSIELLA PNEUMONIAE (bacteria) / Plasmid: PET16B / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P52687, histidine kinase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.3 % / Description: MEROHEDRAL TWINNING ALONG 110 |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: HANGING DROP, VAPOUR DIFFUSION, RESERVOIR: 20MM HEPES,PH 7.5,0.63M NAH2PO4,0.63M KH2PO4, PROTEIN SOLUTION: 15 MG/ML,MIXING RATIO 1:1,TEMPERATURE 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: MPG/DESY, HAMBURG / Beamline: BW6 / Wavelength: 1 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Mar 8, 2006 / Details: MIRROR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→25 Å / Num. obs: 19151 / % possible obs: 97 % / Observed criterion σ(I): 2 / Redundancy: 5.49 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 16.7 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 1.6 % / Rmerge(I) obs: 0.19 / % possible all: 84.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2J80 Resolution: 2→24.6626 Å Details: THE STRUCTURE WAS FIRST REFINED WITH CNS, FOLLOWED BY SHELXL AND FOR BETTER CONVERGENCE WITH PHENIX.REFINE TWIN REFINEMENT PROTOCOL TWIN LAW 1 0 0 0 -1 0 0 0 -1 TWIN FRACTION 0.40
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Refinement step | Cycle: LAST / Resolution: 2→24.6626 Å
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