+Open data
-Basic information
Entry | Database: PDB / ID: 2v16 | ||||||
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Title | Crystal Structure of Renin with Inhibitor 3 | ||||||
Components | RENIN | ||||||
Keywords | HYDROLASE / GLYCOPROTEIN / INHIBITOR-COMPLEX / ASPARTYL PROTEASE / ZYMOGEN / PROTEASE / POLYMORPHISM / ALTERNATIVE SPLICING / HYDROLASE(ACID PROTEINASE) / CLEAVAGE ON PAIR OF BASIC RESIDUES | ||||||
Function / homology | Function and homology information renin / juxtaglomerular apparatus development / mesonephros development / response to cGMP / renin-angiotensin regulation of aldosterone production / drinking behavior / regulation of MAPK cascade / cell maturation / response to immobilization stress / amyloid-beta metabolic process ...renin / juxtaglomerular apparatus development / mesonephros development / response to cGMP / renin-angiotensin regulation of aldosterone production / drinking behavior / regulation of MAPK cascade / cell maturation / response to immobilization stress / amyloid-beta metabolic process / hormone-mediated signaling pathway / angiotensin maturation / Metabolism of Angiotensinogen to Angiotensins / response to cAMP / insulin-like growth factor receptor binding / kidney development / regulation of blood pressure / cellular response to xenobiotic stimulus / male gonad development / apical part of cell / peptidase activity / aspartic-type endopeptidase activity / response to lipopolysaccharide / signaling receptor binding / proteolysis / extracellular space / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Rahuel, J. / Rasetti, V. / Maibaum, J. / Rueger, H. / Goschke, R. / Cohen, N.C. / Stutz, S. / Cumin, F. / Fuhrer, W. / Wood, J.M. / Grutter, M.G. | ||||||
Citation | Journal: Chem.Biol. / Year: 2000 Title: Structure-Based Drug Design: The Discovery of Novel Nonpeptide Orally Active Inhibitors of Human Renin Authors: Rahuel, J. / Rasetti, V. / Maibaum, J. / Rueger, H. / Goschke, R. / Cohen, N.C. / Stutz, S. / Cumin, F. / Fuhrer, W. / Wood, J.M. / Grutter, M.G. #1: Journal: J.Struct.Biol. / Year: 1991 Title: The Crystal Structures of Recombinant Glycosylated Human Renin Alone and in Complex with a Transition State Analog Inhibitor. Authors: Rahuel, J. / Priestle, J.P. / Grutter, M.G. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2v16.cif.gz | 147.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2v16.ent.gz | 99.3 KB | Display | PDB format |
PDBx/mmJSON format | 2v16.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2v16_validation.pdf.gz | 534.7 KB | Display | wwPDB validaton report |
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Full document | 2v16_full_validation.pdf.gz | 818 KB | Display | |
Data in XML | 2v16_validation.xml.gz | 50.8 KB | Display | |
Data in CIF | 2v16_validation.cif.gz | 64.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v1/2v16 ftp://data.pdbj.org/pub/pdb/validation_reports/v1/2v16 | HTTPS FTP |
-Related structure data
Related structure data | 2v0zC 2v10C 2v11C 2v12C 2v13C 1pr7 1pr8 1uhq C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 37267.008 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): CHO / Production host: CRICETULUS GRISEUS (Chinese hamster) / References: UniProt: P00797, renin #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.26 Å3/Da / Density % sol: 62.3 % / Description: NONE |
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Crystal grow | pH: 4.5 / Details: pH 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS FR571 / Wavelength: 1.5418 |
Detector | Type: DELFT INSTRUMENTS / Detector: AREA DETECTOR / Date: Jan 1, 1991 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→10 Å / Num. obs: 23603 / % possible obs: 98 % / Observed criterion σ(I): 2 / Redundancy: 2.53 % / Rmerge(I) obs: 0.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→10 Å / Cross valid method: THROUGHOUT / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 2.8→10 Å
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