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Yorodumi- PDB-2or2: Structure of the W47A/W242A Mutant of Bacterial Phosphatidylinosi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2or2 | ||||||
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Title | Structure of the W47A/W242A Mutant of Bacterial Phosphatidylinositol-Specific Phospholipase C | ||||||
Components | 1-phosphatidylinositol phosphodiesterase | ||||||
Keywords | LYASE / phosphatidylinositol-specific phospholipase C / PI-PLC / Dimer / interfacially impaired / membrane binding / TIM barrel | ||||||
Function / homology | Function and homology information phosphatidylinositol diacylglycerol-lyase / phosphatidylinositol diacylglycerol-lyase activity / phosphoric diester hydrolase activity / lipid catabolic process / extracellular region Similarity search - Function | ||||||
Biological species | Bacillus thuringiensis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.84 Å | ||||||
Authors | Shao, C. / Shi, X. / Wehbi, H. / Zambonelli, C. / Head, J.F. / Seaton, B.A. / Roberts, M.F. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2007 Title: Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C. Authors: Shao, C. / Shi, X. / Wehbi, H. / Zambonelli, C. / Head, J.F. / Seaton, B.A. / Roberts, M.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2or2.cif.gz | 139.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2or2.ent.gz | 110.5 KB | Display | PDB format |
PDBx/mmJSON format | 2or2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/or/2or2 ftp://data.pdbj.org/pub/pdb/validation_reports/or/2or2 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological unit is a dimer. There are two monomers in the asymmetric unit. The two monomers follow a pseudo 2-fold symmetry |
-Components
#1: Protein | Mass: 34060.996 Da / Num. of mol.: 2 / Mutation: W47A, W242A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus thuringiensis (bacteria) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: P08954, phosphatidylinositol diacylglycerol-lyase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.78 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion / pH: 5.4 Details: 15% 2-methyl-2,4-pentanediol, 16% PEG400, pH 5.4, vapor diffusion, temperature 290K |
-Data collection
Diffraction |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X8C / Wavelength: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Detector: CCD / Date: Jan 1, 2003 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.84→50 Å / Num. obs: 60615 / % possible obs: 98.8 % / Redundancy: 4.4 % / Rmerge(I) obs: 0.042 / Χ2: 1.152 / Net I/σ(I): 22.4 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Phasing
Phasing MR | Rfactor: 0.52 / Cor.coef. Fo:Fc: 0.356
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.84→50 Å / σ(F): 0
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Solvent computation | Bsol: 42.412 Å2 | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.23 Å2
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Refinement step | Cycle: LAST / Resolution: 1.84→50 Å
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Refine LS restraints |
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Xplor file |
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