+Open data
-Basic information
Entry | Database: PDB / ID: 1zxg | ||||||
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Title | Solution structure of A219 | ||||||
Components | Immunoglobulin G binding protein A | ||||||
Keywords | Immune System/Protein Binding / IgG-binding / protein A / phage display / Immune System-Protein Binding COMPLEX | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | SOLUTION NMR / CNS 1.1. | ||||||
Authors | He, Y. / Yeh, D.C. / Alexander, P. / Bryan, P.N. / Orban, J. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Solution NMR structures of IgG binding domains with artificially evolved high levels of sequence identity but different folds. Authors: He, Y. / Yeh, D.C. / Alexander, P. / Bryan, P.N. / Orban, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zxg.cif.gz | 361.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zxg.ent.gz | 313.6 KB | Display | PDB format |
PDBx/mmJSON format | 1zxg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zx/1zxg ftp://data.pdbj.org/pub/pdb/validation_reports/zx/1zxg | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Antibody | Mass: 6733.484 Da / Num. of mol.: 1 / Mutation: yes Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Plasmid: pG58-A219 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 DE3 / References: UniProt: Q53759 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: A219 mutant protein, 2 mM ammonium bicarbonate buffer, pH 7.0, 10% D2O Solvent system: 2 mM ammonium bicarbonate buffer, 10% D2O. |
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Sample conditions | Ionic strength: 2 mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: CNS 1.1. / Software ordinal: 1 / Details: simulated annealing. | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: The submitted conformer models are those with the fewest number of constraint violations. Conformers calculated total number: 50 / Conformers submitted total number: 20 |