[English] 日本語
Yorodumi- PDB-1x02: Solution structure of stereo array isotope labeled (SAIL) calmodulin -
+Open data
-Basic information
Entry | Database: PDB / ID: 1x02 | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of stereo array isotope labeled (SAIL) calmodulin | ||||||
Components | calmodulin | ||||||
Keywords | METAL BINDING PROTEIN / SAIL / stereo array isotope labeling | ||||||
Function / homology | Function and homology information enzyme regulator activity / signaling receptor binding / calcium ion binding Similarity search - Function | ||||||
Biological species | Xenopus laevis (African clawed frog) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Kainosho, M. / Torizawa, T. / Terauchi, T. / Ono, A.M. / Guntert, P. | ||||||
Citation | Journal: Nature / Year: 2006 Title: Optimal isotope labelling for NMR protein structure determinations. Authors: Kainosho, M. / Torizawa, T. / Iwashita, Y. / Terauchi, T. / Mei Ono, A. / Guntert, P. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1x02.cif.gz | 883.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1x02.ent.gz | 743.1 KB | Display | PDB format |
PDBx/mmJSON format | 1x02.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1x02_validation.pdf.gz | 339.1 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1x02_full_validation.pdf.gz | 498.2 KB | Display | |
Data in XML | 1x02_validation.xml.gz | 65.5 KB | Display | |
Data in CIF | 1x02_validation.cif.gz | 81.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x0/1x02 ftp://data.pdbj.org/pub/pdb/validation_reports/x0/1x02 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 16721.350 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Xenopus laevis (African clawed frog) / Description: Cell-free protein synthesis / Production host: Escherichia coli (E. coli) / References: UniProt: P62155, UniProt: P0DP33*PLUS |
---|---|
#2: Chemical | ChemComp-CA / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 0.7mM SAIL calmodulin, 5mM MES-d13, 10mM bis-Tris-d19, 5mM CaCl2, 0.1mM NaN3 Solvent system: 90% H2O/10% D2O |
---|---|
Sample conditions | pH: 6.5 / Pressure: AMBIENT / Temperature: 310 K |
-Processing
NMR software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |