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Yorodumi- PDB-1q9f: NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES -
+Open data
-Basic information
Entry | Database: PDB / ID: 1q9f | ||||||
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Title | NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES | ||||||
Components | Outer membrane protein X | ||||||
Keywords | MEMBRANE PROTEIN / OMPX / TROSY / DHPC / DETERGENTS / LIPIDS / MICELLES | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / TORSION ANGLE DYNAMICS (CANDID) | ||||||
Authors | Fernandez, C. / Hilty, C. / Wider, G. / Guntert, P. / Wuthrich, K. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: NMR structure of the integral membrane protein OmpX. Authors: Fernandez, C. / Hilty, C. / Wider, G. / Guntert, P. / Wuthrich, K. #1: Journal: J.Biomol.NMR / Year: 2002 Title: Side chain NMR assignments in the membrane protein OmpX reconstituted in DHPC micelles Authors: Hilty, C. / Fernandez, C. / Wider, G. / Wuthrich, K. #2: Journal: To be Published Title: Stereospecific assignments of the isopropyl methyl groups of the membrane protein OmpX in DHPC micelles Authors: Hilty, C. / Wider, G. / Fernandez, C. / Wuthrich, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1q9f.cif.gz | 866.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1q9f.ent.gz | 753.5 KB | Display | PDB format |
PDBx/mmJSON format | 1q9f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q9/1q9f ftp://data.pdbj.org/pub/pdb/validation_reports/q9/1q9f | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16371.768 Da / Num. of mol.: 1 / Mutation: HIS100A to ASN100A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: OMPX / Plasmid: PET3B / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)PLYSS / References: UniProt: P36546, UniProt: P0A917*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2MM OMPX U-15N,13C,2H; 2MM OMPX U-15N,13C,2H/L,V,ID1- 13CH3; 2MM OMPX U-15N,10%-13C |
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Sample conditions | Ionic strength: 20mM SODIUM PHOSPHATE, 100mM NACL / pH: 6.5 / Pressure: AMBIENT / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: TORSION ANGLE DYNAMICS (CANDID) / Software ordinal: 1 Details: AUTOMATIC IDENTIFICATION OF HYDROGEN BOND CONSTRAINTS | ||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 400 / Conformers submitted total number: 20 |