+Open data
-Basic information
Entry | Database: PDB / ID: 1q38 | ||||||
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Title | Anastellin | ||||||
Components | Fibronectin | ||||||
Keywords | CELL ADHESION / amyloid fibril / anastellin / extracellular matrix / fibronectin type 3 (FN3) domain / dynamic fluctuations / conformational exchange / CHAPS | ||||||
Function / homology | Function and homology information negative regulation of monocyte activation / calcium-independent cell-matrix adhesion / negative regulation of transforming growth factor beta production / Fibronectin matrix formation / Extracellular matrix organization / positive regulation of substrate-dependent cell migration, cell attachment to substrate / neural crest cell migration involved in autonomic nervous system development / peptidase activator activity / fibrinogen complex / peptide cross-linking ...negative regulation of monocyte activation / calcium-independent cell-matrix adhesion / negative regulation of transforming growth factor beta production / Fibronectin matrix formation / Extracellular matrix organization / positive regulation of substrate-dependent cell migration, cell attachment to substrate / neural crest cell migration involved in autonomic nervous system development / peptidase activator activity / fibrinogen complex / peptide cross-linking / integrin activation / ALK mutants bind TKIs / cell-substrate junction assembly / biological process involved in interaction with symbiont / proteoglycan binding / Molecules associated with elastic fibres / extracellular matrix structural constituent / MET activates PTK2 signaling / Syndecan interactions / p130Cas linkage to MAPK signaling for integrins / endodermal cell differentiation / GRB2:SOS provides linkage to MAPK signaling for Integrins / endoplasmic reticulum-Golgi intermediate compartment / Non-integrin membrane-ECM interactions / basement membrane / ECM proteoglycans / Integrin cell surface interactions / positive regulation of axon extension / regulation of ERK1 and ERK2 cascade / collagen binding / Degradation of the extracellular matrix / Integrin signaling / substrate adhesion-dependent cell spreading / extracellular matrix / cell-matrix adhesion / platelet alpha granule lumen / acute-phase response / integrin-mediated signaling pathway / Cell surface interactions at the vascular wall / Post-translational protein phosphorylation / regulation of protein phosphorylation / wound healing / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / response to wounding / positive regulation of fibroblast proliferation / integrin binding / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / GPER1 signaling / Signaling by BRAF and RAF1 fusions / Signaling by ALK fusions and activated point mutants / Platelet degranulation / nervous system development / heart development / heparin binding / regulation of cell shape / protease binding / Interleukin-4 and Interleukin-13 signaling / angiogenesis / collagen-containing extracellular matrix / blood microparticle / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / cell adhesion / apical plasma membrane / endoplasmic reticulum lumen / signaling receptor binding / positive regulation of cell population proliferation / positive regulation of gene expression / extracellular space / extracellular exosome / extracellular region / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / Aria protocol | ||||||
Authors | Briknarova, K. / Akerman, M.E. / Hoyt, D.W. / Ruoslahti, E. / Ely, K.R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: Anastellin, an FN3 fragment with fibronectin polymerization activity, resembles amyloid fibril precursors Authors: Briknarova, K. / Akerman, M.E. / Hoyt, D.W. / Ruoslahti, E. / Ely, K.R. #1: Journal: Nature / Year: 1994 Title: Superfibronectin is a functionally distinct form of fibronectin Authors: Morla, A. / Zhang, Z. / Ruoslahti, E. #2: Journal: NAT.MED. (N.Y.) / Year: 1996 Title: A polymeric form of fibronectin has antimetastatic effects against multiple tumor types Authors: Pasqualini, R. / Bourdoulous, S. / Koivunen, E. / Woods Jr., V.L. / Ruoslahti, E. #3: Journal: Proc.Natl.Acad.Sci.USA / Year: 2001 Title: A fibronectin fragment inhibits tumor growth, angiogenesis, and metastasis Authors: Yi, M. / Ruoslahti, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1q38.cif.gz | 958.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1q38.ent.gz | 808.6 KB | Display | PDB format |
PDBx/mmJSON format | 1q38.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1q38_validation.pdf.gz | 343.6 KB | Display | wwPDB validaton report |
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Full document | 1q38_full_validation.pdf.gz | 622.6 KB | Display | |
Data in XML | 1q38_validation.xml.gz | 53.7 KB | Display | |
Data in CIF | 1q38_validation.cif.gz | 86.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q3/1q38 ftp://data.pdbj.org/pub/pdb/validation_reports/q3/1q38 | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10175.405 Da / Num. of mol.: 1 / Fragment: Type 3 (FN3) domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FN1 OR FN / Production host: Escherichia coli (E. coli) / References: UniProt: P02751 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 150 / pH: 7 / Pressure: ambient / Temperature: 298 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Aria protocol / Software ordinal: 1 / Details: multiple Aria runs | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 35 |