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Yorodumi- PDB-1pvu: THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1pvu | ||||||
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Title | THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV | ||||||
Components | Pvu II | ||||||
Keywords | TYPE II RESTRICTION ENDONUCLEASE | ||||||
Function / homology | Function and homology information type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Proteus vulgaris (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.4 Å | ||||||
Authors | Vlassi, M. / Athanasiadis, A. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1994 Title: Crystal structure of PvuII endonuclease reveals extensive structural homologies to EcoRV. Authors: Athanasiadis, A. / Vlassi, M. / Kotsifaki, D. / Tucker, P.A. / Wilson, K.S. / Kokkinidis, M. #1: Journal: J.Mol.Biol. / Year: 1991 Title: Purification,Crystallization and Preliminary X-Ray Diffraction Studies of the PvuII Endonuclease Authors: Athanasiadis, A. / Kokkinidis, M. #2: Journal: Nucleic Acids Res. / Year: 1990 Title: Complete Nucleotide Sequence of the PvuII Restriction Enzyme Gene from Proteus Vulgaris Authors: Athanasiadis, A. / Gregoriu, M. / Thanos, D. / Kokkinidis, M. / Papamatheakis, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pvu.cif.gz | 74.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pvu.ent.gz | 57.5 KB | Display | PDB format |
PDBx/mmJSON format | 1pvu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1pvu_validation.pdf.gz | 375.4 KB | Display | wwPDB validaton report |
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Full document | 1pvu_full_validation.pdf.gz | 384.9 KB | Display | |
Data in XML | 1pvu_validation.xml.gz | 8.6 KB | Display | |
Data in CIF | 1pvu_validation.cif.gz | 12.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pv/1pvu ftp://data.pdbj.org/pub/pdb/validation_reports/pv/1pvu | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.732901, -0.680335, -0.000615), Vector: |
-Components
#1: Protein | Mass: 18239.799 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Proteus vulgaris (bacteria) References: UniProt: P23657, type II site-specific deoxyribonuclease #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.61 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 18 ℃ / pH: 5 / Method: vapor diffusion, hanging drop / Details: Athanasiadis, A., (1991) J.Mol.Biol., 222, 451. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.3 Å / Num. obs: 16315 / % possible obs: 96.3 % / Rmerge(I) obs: 0.076 |
-Processing
Software |
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Refinement | Resolution: 2.4→6 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 2.4→6 Å
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Refine LS restraints |
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