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- PDB-1pru: PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING -

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Basic information

Entry
Database: PDB / ID: 1pru
TitlePURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING
ComponentsPURINE REPRESSOR
KeywordsDNA BINDING PROTEIN / PURINE REPRESSOR / DNA-BINDING PROTEIN
Function / homology
Function and homology information


guanine binding / negative regulation of purine nucleotide biosynthetic process / purine nucleotide biosynthetic process / DNA-binding transcription repressor activity / transcription cis-regulatory region binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / protein homodimerization activity / cytosol
Similarity search - Function
Transcription regulator HTH, PurR / Transcriptional regulator LacI/GalR-like, sensor domain / LacI-type HTH domain signature. / Periplasmic binding protein-like domain / LacI-type HTH domain / Bacterial regulatory proteins, lacI family / LacI-type HTH domain profile. / helix_turn _helix lactose operon repressor / lambda repressor-like DNA-binding domains / 434 Repressor (Amino-terminal Domain) ...Transcription regulator HTH, PurR / Transcriptional regulator LacI/GalR-like, sensor domain / LacI-type HTH domain signature. / Periplasmic binding protein-like domain / LacI-type HTH domain / Bacterial regulatory proteins, lacI family / LacI-type HTH domain profile. / helix_turn _helix lactose operon repressor / lambda repressor-like DNA-binding domains / 434 Repressor (Amino-terminal Domain) / Lambda repressor-like, DNA-binding domain superfamily / Periplasmic binding protein-like I / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
HTH-type transcriptional repressor PurR
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodSOLUTION NMR
AuthorsNagadoi, A. / Morikawa, S. / Nakamura, H. / Enari, M. / Kobayashi, K. / Yamamoto, H. / Sampei, G. / Mizobuchi, K. / Schumacher, M.A. / Brennan, R.G. / Nishimura, Y.
Citation
Journal: Structure / Year: 1995
Title: Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain.
Authors: Nagadoi, A. / Morikawa, S. / Nakamura, H. / Enari, M. / Kobayashi, K. / Yamamoto, H. / Sampei, G. / Mizobuchi, K. / Schumacher, M.A. / Brennan, R.G. / Nishimura, Y.
#1: Journal: Science / Year: 1994
Title: Crystal Structure of LacI Member, PurR, Bound to DNA: Minor Groove Binding by Alpha Helices
Authors: Schumacher, M.A. / Choi, K.Y. / Zalkin, H. / Brennan, R.G.
History
DepositionMay 8, 1995-
Revision 1.0Mar 8, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 22, 2012Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PURINE REPRESSOR


Theoretical massNumber of molelcules
Total (without water)6,1791
Polymers6,1791
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: Protein PURINE REPRESSOR


Mass: 6179.133 Da / Num. of mol.: 1 / Fragment: DNA-BINDING
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: PAR2156NCOI / Species (production host): Escherichia coli / Gene (production host): PURR / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P0ACP7

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other

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Processing

SoftwareName: AMBER / Classification: refinement
NMR software
NameDeveloperClassification
EMBOSSNAKAI,KIDERA,NAKAMURArefinement
PRESTOMORIKAMI,NAKAI,KIDERA,SAITO,NAKAMURArefinement
NMR ensembleConformers submitted total number: 1

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