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Yorodumi- PDB-1pnt: CRYSTAL STRUCTURE OF BOVINE HEART PHOSPHOTYROSYL PHOSPHATASE AT 2... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1pnt | ||||||
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Title | CRYSTAL STRUCTURE OF BOVINE HEART PHOSPHOTYROSYL PHOSPHATASE AT 2.2 ANGSTROMS RESOLUTION | ||||||
Components | ACID PHOSPHATASE | ||||||
Keywords | HYDROLASE | ||||||
Function / homology | Function and homology information acid phosphatase / acid phosphatase activity / non-membrane spanning protein tyrosine phosphatase activity / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity / cytoplasm Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.2 Å | ||||||
Authors | Zhang, M. / Van Etten, R.L. / Stauffacher, C.V. | ||||||
Citation | Journal: Biochemistry / Year: 1994 Title: Crystal structure of bovine heart phosphotyrosyl phosphatase at 2.2-A resolution. Authors: Zhang, M. / Van Etten, R.L. / Stauffacher, C.V. #1: Journal: J.Mol.Biol. / Year: 1994 Title: Crystallization and Preliminary X-Ray Analysis of the Low Molecular Weight Phosphotyrosyl Protein Phosphatase from Bovine Heart Authors: Zhang, M. / Van Etten, R.L. / Lawrence, C.M. / Stauffacher, C.V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pnt.cif.gz | 41.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pnt.ent.gz | 29.4 KB | Display | PDB format |
PDBx/mmJSON format | 1pnt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1pnt_validation.pdf.gz | 381.3 KB | Display | wwPDB validaton report |
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Full document | 1pnt_full_validation.pdf.gz | 389 KB | Display | |
Data in XML | 1pnt_validation.xml.gz | 5.8 KB | Display | |
Data in CIF | 1pnt_validation.cif.gz | 7.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pn/1pnt ftp://data.pdbj.org/pub/pdb/validation_reports/pn/1pnt | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES 1 - 3 ARE MODELED IN WEAKER DENSITY AND HAVE CORRESPONDINGLY HIGHER TEMPERATURE FACTORS. |
-Components
#1: Protein | Mass: 17946.326 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / References: UniProt: P11064, acid phosphatase |
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#2: Chemical | ChemComp-PO4 / |
Compound details | THE ACTIVE SITE SEQUENCE MOTIF FOR ALL MEMBERS OF THE TYROSINE PHOSPHATASE FAMILY IS CXXXXXRS/T. ...THE ACTIVE SITE SEQUENCE MOTIF FOR ALL MEMBERS OF THE TYROSINE PHOSPHATAS |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.35 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion, hanging drop / Details: Zhang, M., (1994) J.Mol.Biol., 238, 281. | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.2 Å / % possible obs: 90 % / Redundancy: 3.1 % / Rmerge(I) obs: 0.06 |
-Processing
Software |
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Refinement | Resolution: 2.2→20 Å / σ(F): 0 Details: RESIDUES 1 - 3 ARE MODELED IN WEAKER DENSITY AND HAVE CORRESPONDINGLY HIGHER TEMPERATURE FACTORS.
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Refinement step | Cycle: LAST / Resolution: 2.2→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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