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Yorodumi- PDB-1pga: TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1pga | ||||||
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Title | TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON WITH NMR | ||||||
Components | PROTEIN G | ||||||
Keywords | IMMUNOGLOBULIN BINDING PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Streptococcus sp. GX7805 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.07 Å | ||||||
Authors | Gallagher, T. / Alexander, P. / Bryan, P. / Gilliland, G.L. | ||||||
Citation | Journal: Biochemistry / Year: 1994 Title: Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR. Authors: Gallagher, T. / Alexander, P. / Bryan, P. / Gilliland, G.L. #1: Journal: Biochemistry / Year: 1992 Title: 1.67 Angstroms X-Ray Structure of the B2 Immunoglobulin-Binding Domain of Streptoccocal Protein G and Comparison to the NMR Structure of the B1 Domain Authors: Achari, A. / Hale, S.P. / Howard, A.J. / Clore, G.M. / Gronenborn, A.M. / Hardman, K.D. / Whitlow, M. #2: Journal: Science / Year: 1991 Title: A Novel, Highly Stable Fold of the Immunoglobulin Binding Domain of Streptococcal Protein G Authors: Gronenborn, A.M. / Filpula, D.R. / Essig, N.Z. / Achari, A. / Whitlow, M. / Wingfield, P.T. / Clore, G.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pga.cif.gz | 20.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pga.ent.gz | 12.8 KB | Display | PDB format |
PDBx/mmJSON format | 1pga.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1pga_validation.pdf.gz | 364.5 KB | Display | wwPDB validaton report |
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Full document | 1pga_full_validation.pdf.gz | 364.5 KB | Display | |
Data in XML | 1pga_validation.xml.gz | 2.5 KB | Display | |
Data in CIF | 1pga_validation.cif.gz | 3.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pg/1pga ftp://data.pdbj.org/pub/pdb/validation_reports/pg/1pga | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 6201.784 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus sp. GX7805 (bacteria) / References: UniProt: P06654 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.92 Å3/Da / Density % sol: 35.95 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 18 ℃ / pH: 4.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.04 Å / Num. obs: 2701 / Rmerge(I) obs: 0.07 |
Reflection shell | *PLUS Mean I/σ(I) obs: 6.4 |
-Processing
Software |
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Refinement | Resolution: 2.07→6 Å / σ(I): 1 /
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Refinement step | Cycle: LAST / Resolution: 2.07→6 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.174 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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