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Yorodumi- PDB-1h4z: Structure of the Anti-Sigma Factor Antagonist SpoIIAA in its Unph... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1h4z | ||||||
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Title | Structure of the Anti-Sigma Factor Antagonist SpoIIAA in its Unphosphorylated Form | ||||||
Components | ANTI-SIGMA F FACTOR ANTAGONIST | ||||||
Keywords | CELL DIFFERENTIATION / PHOSPHORYLATION / SIGMA FACTOR / SPORULATION | ||||||
Function / homology | Function and homology information anti-sigma factor antagonist activity / antisigma factor binding / sporulation resulting in formation of a cellular spore Similarity search - Function | ||||||
Biological species | BACILLUS SPHAERICUS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.74 Å | ||||||
Authors | Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
Citation | Journal: Structure / Year: 2001 Title: Structure of the Bacillus Cell Fate Determinant Spoiiaa in Phosphorylated and Unphosphorylated Forms Authors: Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1h4z.cif.gz | 33.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1h4z.ent.gz | 22.8 KB | Display | PDB format |
PDBx/mmJSON format | 1h4z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h4/1h4z ftp://data.pdbj.org/pub/pdb/validation_reports/h4/1h4z | HTTPS FTP |
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-Related structure data
Related structure data | 1h4xSC 1h4yC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13125.223 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACILLUS SPHAERICUS (bacteria) / Strain: ATCC14577 / Plasmid: PET28A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: O32723*PLUS |
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#2: Water | ChemComp-HOH / |
Sequence details | THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUSS STRAIN ATCC14577, IS SIMILAR TO THE SWISSPROT ENTRY ...THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUS |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 53.07 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 9 / Details: 2M MGCL2, 0.1M BICINE (PH 9.0) | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / pH: 8.5 / Method: vapor diffusion, hanging drop / Details: Seavers, P.R., (2001) Acta Crystallogr., D57, 292. | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-3 / Wavelength: 0.93 |
Detector | Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.93 Å / Relative weight: 1 |
Reflection | Resolution: 2.74→20 Å / Num. obs: 4022 / % possible obs: 99.8 % / Redundancy: 6.7 % / Rmerge(I) obs: 0.072 / Net I/σ(I): 23.5 |
Reflection shell | Resolution: 2.74→2.84 Å / Rmerge(I) obs: 0.342 / Mean I/σ(I) obs: 5.3 / % possible all: 99.7 |
Reflection | *PLUS Num. measured all: 26991 |
Reflection shell | *PLUS % possible obs: 99.7 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1H4X Resolution: 2.74→20 Å / SU B: 14.33 / SU ML: 0.301 / Cross valid method: THROUGHOUT / ESU R: 1.068 / ESU R Free: 0.404 / Details: NONE
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Refinement step | Cycle: LAST / Resolution: 2.74→20 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.25 / Rfactor Rfree: 0.305 / Rfactor Rwork: 0.251 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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