+Open data
-Basic information
Entry | Database: PDB / ID: 1fla | ||||||
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Title | CLOSTRIDIUM BEIJERINCKII FLAVODOXIN MUTANT: G57D REDUCED | ||||||
Components | FLAVODOXIN | ||||||
Keywords | ELECTRON TRANSPORT / FLAVOPROTEIN / FMN | ||||||
Function / homology | Function and homology information oxidoreductase activity, acting on NAD(P)H / FMN binding / electron transfer activity Similarity search - Function | ||||||
Biological species | Clostridium beijerinckii (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Ludwig, M.L. / Pattridge, K.A. / Metzger, A.L. / Dixon, M.M. / Eren, M. / Feng, Y. / Swenson, R. | ||||||
Citation | Journal: Biochemistry / Year: 1997 Title: Control of oxidation-reduction potentials in flavodoxin from Clostridium beijerinckii: the role of conformation changes. Authors: Ludwig, M.L. / Pattridge, K.A. / Metzger, A.L. / Dixon, M.M. / Eren, M. / Feng, Y. / Swenson, R.P. #1: Journal: Flavins and Flavoproteins 1993 : Proceedings of the Eleventh International Symposium, Nagoya (Japan) July 27-31, 1993 Year: 1994 Title: Cis-Trans Isomerization of the 57-58 Peptide in Crystalline Flavodoxins from C. Beijerinckii Authors: Ludwig, M.L. / Dixon, M.M. / Pattridge, K.A. / Swenson, R.P. #2: Journal: Chemistry and Biochemistry of Flavoenzymes / Year: 1992 Title: Structure and Redox Properties of Clostridial Flavodoxin Authors: Ludwig, M.L. / Luschinsky, C.L. #3: Journal: Flavins and Flavoproteins 1990 : Proceedings of the Tenth International Symposium, Como, Italy, July 15-20, 1990 Year: 1991 Title: Structural Characterization of Site Mutants of Clostridial Flavodoxin Authors: Ludwig, M.L. / Pattridge, K.A. / Eren, M. / Swenson, R.P. #4: Journal: Biochemistry / Year: 1990 Title: Structure and Oxidation-Reduction Behavior of 1-Deaza-Fmn Flavodoxins: Modulation of Redox Potentials in Flavodoxins Authors: Ludwig, M.L. / Schopfer, L.M. / Metzger, A.L. / Pattridge, K.A. / Massey, V. #5: Journal: J.Mol.Biol. / Year: 1977 Title: Structure of the Semiquinone Form of Flavodoxin from Clostridium Mp. Extension of 1.8 A Resolution and Some Comparisons with the Oxidized State Authors: Smith, W.W. / Burnett, R.M. / Darling, G.D. / Ludwig, M.L. #6: Journal: FLAVINS AND FLAVOPROTEINS / Year: 1976 Title: The Structure of Clostridium Mp Flavodoxin as a Function of Oxidation State, Some Comparisons of the Fmn-Binding Sites in Oxidized, Semiquinone and Reduced Forms Authors: Ludwig, M.L. / Burnett, R.M. / Darling, G.D. / Jordan, S.R. / Kendall, D.S. / Smith, W.W. #7: Journal: J.Biol.Chem. / Year: 1974 Title: The Structure of the Oxidized Form of Clostridial Flavodoxin at 1.9-A Resolution Authors: Burnett, R.M. / Darling, G.D. / Kendall, D.S. / Lequesne, M.E. / Mayhew, S.G. / Smith, W.W. / Ludwig, M.L. #8: Journal: Proc.Natl.Acad.Sci.USA / Year: 1972 Title: Structure of the Radical Form of Clostridial Flavodoxin: A New Molecular Model Authors: Andersen, R.D. / Apgar, P.A. / Burnett, R.M. / Darling, G.D. / Lequesne, M.E. / Mayhew, S.G. / Ludwig, M.L. #9: Journal: J.Biol.Chem. / Year: 1969 Title: The Structure of a Clostridial Flavodoxin. I. Crystallographic Characterization of the Oxidized and Semiquinone Forms Authors: Ludwig, M.L. / Andersen, R.D. / Mayhew, S.G. / Massey, V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fla.cif.gz | 42.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fla.ent.gz | 29.7 KB | Display | PDB format |
PDBx/mmJSON format | 1fla.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fla_validation.pdf.gz | 453.3 KB | Display | wwPDB validaton report |
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Full document | 1fla_full_validation.pdf.gz | 454.7 KB | Display | |
Data in XML | 1fla_validation.xml.gz | 4.5 KB | Display | |
Data in CIF | 1fla_validation.cif.gz | 6.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fl/1fla ftp://data.pdbj.org/pub/pdb/validation_reports/fl/1fla | HTTPS FTP |
-Related structure data
Related structure data | 1fldC 1flnC 1fvxC 2faxC 2fdxC 2flvC 2foxC 2fvxC 3nllC 4nllC 4nulC 5nllC 5nulC 5ullC 6nulC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15402.316 Da / Num. of mol.: 1 / Mutation: G57D Source method: isolated from a genetically manipulated source Details: REDUCED / Source: (gene. exp.) Clostridium beijerinckii (bacteria) / Cell line: XL1-BLUE / Cell line (production host): XL1-BLUE / Production host: Escherichia coli (E. coli) / References: UniProt: P00322 |
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#2: Chemical | ChemComp-FMN / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.48 % | ||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6.8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Detector: AREA DETECTOR / Date: Mar 1, 1991 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→10 Å / Num. obs: 13988 / % possible obs: 99.5 % / Observed criterion σ(I): 0 |
Reflection | *PLUS Rmerge(I) obs: 0.0508 |
-Processing
Software |
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Refinement | Resolution: 1.9→10 Å / σ(F): 0
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Displacement parameters | Biso mean: 18.93 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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