+Open data
-Basic information
Entry | Database: PDB / ID: 1eof | ||||||
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Title | CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN | ||||||
Components | POTASSIUM CHANNEL KV1.1 | ||||||
Keywords | MEMBRANE PROTEIN / POTASSIUM CHANNELS / APLYSIA KV1.1 / PROTON TRANSPORT | ||||||
Function / homology | Function and homology information delayed rectifier potassium channel activity / voltage-gated potassium channel complex / protein homooligomerization Similarity search - Function | ||||||
Biological species | Aplysia californica (California sea hare) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.38 Å | ||||||
Authors | Nanao, M.H. / Cushman, S.J. / Jahng, A.W. / DeRubeis, D. / Choe, S. / Pfaffinger, P.J. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Voltage dependent activation of potassium channels is coupled to T1 domain structure. Authors: Cushman, S.J. / Nanao, M.H. / Jahng, A.W. / DeRubeis, D. / Choe, S. / Pfaffinger, P.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eof.cif.gz | 32.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eof.ent.gz | 22.7 KB | Display | PDB format |
PDBx/mmJSON format | 1eof.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1eof_validation.pdf.gz | 357.3 KB | Display | wwPDB validaton report |
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Full document | 1eof_full_validation.pdf.gz | 357.8 KB | Display | |
Data in XML | 1eof_validation.xml.gz | 3.4 KB | Display | |
Data in CIF | 1eof_validation.cif.gz | 5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eo/1eof ftp://data.pdbj.org/pub/pdb/validation_reports/eo/1eof | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 12054.428 Da / Num. of mol.: 1 / Fragment: SHAKER T1 DOMAIN / Mutation: N136A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aplysia californica (California sea hare) Plasmid: PET20 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: Q16968 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.18 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 30% Isopropanol, .1 M Hepes 7.5, .2 M MgCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion / Details: Bixby, K.A., (1999) Nature Struct. Biol., 6, 38. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: MACSCIENCE / Wavelength: 1.5418 |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: Feb 9, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.38→35 Å / Num. all: 5586 / Num. obs: 5586 / % possible obs: 89 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 14 % / Biso Wilson estimate: 21.2 Å2 / Rmerge(I) obs: 0.069 / Net I/σ(I): 11.9 |
Reflection shell | Resolution: 2.38→2.46 Å / Redundancy: 8.2 % / Rmerge(I) obs: 0.256 / % possible all: 100 |
Reflection | *PLUS % possible obs: 98 % / Num. measured all: 81032 |
-Processing
Software |
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Refinement | Resolution: 2.38→14.9 Å / Rfactor Rfree error: 0.017 / Data cutoff high absF: 1398820.28 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 3 / σ(I): 0 / Stereochemistry target values: ENGH & HUBER
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 34.02 Å2 / ksol: 0.3385 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.38→14.9 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.38→2.53 Å / Rfactor Rfree error: 0.064 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 3 / % reflection Rfree: 4.8 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 27.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.202 / % reflection Rfree: 1.4 % / Rfactor Rwork: 0.28 |