+Open data
-Basic information
Entry | Database: PDB / ID: 1dfj | ||||||
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Title | RIBONUCLEASE INHIBITOR COMPLEXED WITH RIBONUCLEASE A | ||||||
Components |
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Keywords | COMPLEX (ENDONUCLEASE/INHIBITOR) / COMPLEX (RIBONUCLEASE-INHIBITOR) / RIBONUCLEASE / HYDROLASE / LEUCINE-RICH REPEATS / COMPLEX (ENDONUCLEASE-INHIBITOR) COMPLEX | ||||||
Function / homology | Function and homology information ribonuclease inhibitor activity / angiogenin-PRI complex / regulation of Arp2/3 complex-mediated actin nucleation / pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / regulation of angiogenesis / regulation of inflammatory response / cell migration / lamellipodium ...ribonuclease inhibitor activity / angiogenin-PRI complex / regulation of Arp2/3 complex-mediated actin nucleation / pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / regulation of angiogenesis / regulation of inflammatory response / cell migration / lamellipodium / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region / nucleoplasm / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | ||||||
Authors | Kobe, B. / Deisenhofer, J. | ||||||
Citation | Journal: Nature / Year: 1995 Title: A structural basis of the interactions between leucine-rich repeats and protein ligands. Authors: Kobe, B. / Deisenhofer, J. #1: Journal: To be Published Title: Mechanism of Ribonuclease Inhibition by Ribonuclease Inhibitor Protein Based on the Crystal Structure of its Complex with Ribonuclease A Authors: Kobe, B. / Deisenhofer, J. #2: Journal: J.Mol.Biol. / Year: 1994 Title: Complex between Bovine Ribonuclease A and Porcine Ribonuclease Inhibitor Crystallizes in a Similar Unit Cell as Free Ribonuclease Inhibitor Authors: Kobe, B. / Ma, Z. / Deisenhofer, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dfj.cif.gz | 116.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dfj.ent.gz | 94.6 KB | Display | PDB format |
PDBx/mmJSON format | 1dfj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1dfj_validation.pdf.gz | 438.4 KB | Display | wwPDB validaton report |
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Full document | 1dfj_full_validation.pdf.gz | 453.5 KB | Display | |
Data in XML | 1dfj_validation.xml.gz | 23.4 KB | Display | |
Data in CIF | 1dfj_validation.cif.gz | 32.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/df/1dfj ftp://data.pdbj.org/pub/pdb/validation_reports/df/1dfj | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13708.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / Tissue: PANCREAS / References: UniProt: P61823, EC: 3.1.27.5 |
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#2: Protein | Mass: 49092.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: PURCHASED FROM SIGMA (TYPE XII-A) / Source: (natural) Sus scrofa (pig) / Tissue: LIVER / References: UniProt: P10775 |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 59.88 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 21 ℃ / pH: 7 / Method: unknown / Details: Kobe, B., (1994) J.Mol.Biol., 241, 288. | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 18859 / % possible obs: 71.4 % / Observed criterion σ(I): 1 / Redundancy: 4.2 % / Rmerge(I) obs: 0.1 |
Reflection | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 40 Å / Num. measured all: 111046 |
-Processing
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Refinement | Resolution: 2.5→40 Å / σ(F): 1 /
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Refine analyze | Luzzati coordinate error obs: 0.32 Å / Luzzati sigma a obs: 0.57 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→40 Å
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Refine LS restraints |
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