+Open data
-Basic information
Entry | Database: PDB / ID: 1d1h | ||||||
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Title | SOLUTION STRUCTURE OF HANATOXIN 1 | ||||||
Components | HANATOXIN TYPE 1 | ||||||
Keywords | TOXIN / CYSTINE KNOT | ||||||
Function / homology | Huwentoxin-1 family / Ion channel inhibitory toxin / ion channel inhibitor activity / toxin activity / extracellular region / Kappa-theraphotoxin-Gr1a Function and homology information | ||||||
Biological species | Grammostola rosea (Chilean rose tarantula) | ||||||
Method | SOLUTION NMR / DYNAMICAL SIMULATED ANNEALING | ||||||
Authors | Takahashi, H. / Kim, J.I. / Sato, K. / Swartz, K.J. / Shimada, I. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Solution structure of hanatoxin1, a gating modifier of voltage-dependent K(+) channels: common surface features of gating modifier toxins. Authors: Takahashi, H. / Kim, J.I. / Min, H.J. / Sato, K. / Swartz, K.J. / Shimada, I. #1: Journal: Neuron / Year: 1995 Title: An inhibitor of the Kv2.1 potassium channel isolated from the venom of a Chilean Tarantula Authors: Swartz, K.J. / MacKinnon, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1d1h.cif.gz | 224.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1d1h.ent.gz | 193.1 KB | Display | PDB format |
PDBx/mmJSON format | 1d1h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d1/1d1h ftp://data.pdbj.org/pub/pdb/validation_reports/d1/1d1h | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4127.771 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Grammostola rosea (Chilean rose tarantula) Tissue: VENOM / References: UniProt: P56852 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: THIS STRUCTURE WAS DETERMINED USING STANDARD 2D HOMONUCLEAR TECHNIQUES. |
-Sample preparation
Details | Contents: 2MM HANATOXIN1 |
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Sample conditions | Ionic strength: 0 / pH: 3.4 / Pressure: 1 atm / Temperature: 300 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: DYNAMICAL SIMULATED ANNEALING / Software ordinal: 1 Details: THE STRUCTURES ARE BASED ON A TOTAL OF 539 RESTRAINTS, 509 ARE NOE-DERIVED DISTANCE CONSTRAINTS, 19 DIHEDRAL ANGLE RESTRAINTS,11 DISTANCE RESTRAINTS FROM HYDROGEN BONDS AND DISULFIDE BONDS. | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 21 |