+Open data
-Basic information
Entry | Database: PDB / ID: 1cic | ||||||
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Title | IDIOTOPE-ANTI-IDIOTOPE FAB-FAB COMPLEX; D1.3-E225 | ||||||
Components | (PROTEIN (IG HEAVY CHAIN V REGIONS)) x 4 | ||||||
Keywords | IMMUNOGLOBULIN / FAB COMPLEX / IDIOTOPE / ANTI-IDIOTOPE | ||||||
Function / homology | Function and homology information Initial triggering of complement / Classical antibody-mediated complement activation / FCGR activation / Role of phospholipids in phagocytosis / Regulation of Complement cascade / Regulation of actin dynamics for phagocytic cup formation / humoral immune response mediated by circulating immunoglobulin / phagocytosis, recognition / positive regulation of type IIa hypersensitivity / positive regulation of type I hypersensitivity ...Initial triggering of complement / Classical antibody-mediated complement activation / FCGR activation / Role of phospholipids in phagocytosis / Regulation of Complement cascade / Regulation of actin dynamics for phagocytic cup formation / humoral immune response mediated by circulating immunoglobulin / phagocytosis, recognition / positive regulation of type IIa hypersensitivity / positive regulation of type I hypersensitivity / antibody-dependent cellular cytotoxicity / phagocytosis, engulfment / immunoglobulin mediated immune response / immunoglobulin complex, circulating / immunoglobulin receptor binding / positive regulation of phagocytosis / B cell differentiation / complement activation, classical pathway / antigen binding / positive regulation of immune response / antibacterial humoral response / defense response to bacterium / external side of plasma membrane / extracellular space / extracellular region / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Bentley, G.A. | ||||||
Citation | Journal: Nature / Year: 1990 Title: Three-dimensional structure of an idiotope-anti-idiotope complex. Authors: Bentley, G.A. / Boulot, G. / Riottot, M.M. / Poljak, R.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cic.cif.gz | 176.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cic.ent.gz | 139.8 KB | Display | PDB format |
PDBx/mmJSON format | 1cic.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1cic_validation.pdf.gz | 446.7 KB | Display | wwPDB validaton report |
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Full document | 1cic_full_validation.pdf.gz | 482 KB | Display | |
Data in XML | 1cic_validation.xml.gz | 35.8 KB | Display | |
Data in CIF | 1cic_validation.cif.gz | 49.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ci/1cic ftp://data.pdbj.org/pub/pdb/validation_reports/ci/1cic | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 23761.291 Da / Num. of mol.: 1 / Fragment: FAB IMMUNOGLOBULIN FRAGMENT / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Strain: BALB-C / References: UniProt: Q9R1A5, UniProt: P01837*PLUS |
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#2: Antibody | Mass: 23157.723 Da / Num. of mol.: 1 / Fragment: FAB IMMUNOGLOBULIN FRAGMENT / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Strain: BALB-C / References: UniProt: P01867 |
#3: Antibody | Mass: 23678.049 Da / Num. of mol.: 1 / Fragment: FAB IMMUNOGLOBULIN FRAGMENT / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Strain: BALB-C / References: UniProt: Q9R1A5, UniProt: I6L958*PLUS |
#4: Antibody | Mass: 23398.234 Da / Num. of mol.: 1 / Fragment: FAB IMMUNOGLOBULIN FRAGMENT / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Strain: BALB-C / References: UniProt: P01869 |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 55.84 % |
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Crystal grow | pH: 4.9 Details: 12% PEG 800, 75 MILLIMOLAR SODIUM ACETATE PH 4.85 10MGM/ML PROTEIN CONCENTRATION , pH 4.9 |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 / Wavelength: 1.5418 Å |
Detector | Detector: AREA DETECTOR / Date: Nov 1, 1987 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→13.7 Å / Num. obs: 33526 / % possible obs: 97 % / Redundancy: 2.84 % / Rmerge(I) obs: 0.076 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: FAB D1.3 Resolution: 2.5→7 Å / σ(F): 2.5 /
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Refine analyze | Luzzati coordinate error obs: 0.3 Å / Luzzati d res low obs: 7 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→7 Å
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Refine LS restraints |
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Software | *PLUS Name: PROFFT / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.179 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |