+Open data
-Basic information
Entry | Database: PDB / ID: 1bxw | ||||||
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Title | OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN | ||||||
Components | PROTEIN (OUTER MEMBRANE PROTEIN A) | ||||||
Keywords | MEMBRANE PROTEIN / OUTER MEMBRANE / TRANSMEMBRANE PROTEIN | ||||||
Function / homology | Function and homology information outer membrane protein complex / monoatomic ion transmembrane transporter activity / detection of virus / outer membrane / porin activity / pore complex / monoatomic ion transport / cell outer membrane / outer membrane-bounded periplasmic space / symbiont entry into host cell ...outer membrane protein complex / monoatomic ion transmembrane transporter activity / detection of virus / outer membrane / porin activity / pore complex / monoatomic ion transport / cell outer membrane / outer membrane-bounded periplasmic space / symbiont entry into host cell / DNA damage response / identical protein binding / membrane Similarity search - Function | ||||||
Biological species | Escherichia coli BL21 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MIRAS / Resolution: 2.5 Å | ||||||
Authors | Schulz, G.E. / Pautsch, A. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1998 Title: Structure of the outer membrane protein A transmembrane domain. Authors: Pautsch, A. / Schulz, G.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bxw.cif.gz | 47.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bxw.ent.gz | 34 KB | Display | PDB format |
PDBx/mmJSON format | 1bxw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1bxw_validation.pdf.gz | 540.7 KB | Display | wwPDB validaton report |
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Full document | 1bxw_full_validation.pdf.gz | 553.7 KB | Display | |
Data in XML | 1bxw_validation.xml.gz | 11.3 KB | Display | |
Data in CIF | 1bxw_validation.cif.gz | 14.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bx/1bxw ftp://data.pdbj.org/pub/pdb/validation_reports/bx/1bxw | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18892.898 Da / Num. of mol.: 1 / Fragment: TRANSMEMBRANE DOMAIN / Mutation: F23L, Q34K, K107Y Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli BL21(DE3) (bacteria) / Species: Escherichia coli / Strain: BL21DE3 / Gene: OMPA / Plasmid: PET3B-171 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21DE3 / References: UniProt: P0A910 |
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#2: Chemical | ChemComp-C8E / ( |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.7 Å3/Da / Density % sol: 66.7 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 5 Details: 10 % PEG-8000 10 % MPD 0.05 M POTASSIUM PHOSPHATE PH 5.0 | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.1 / Method: vapor diffusion, hanging dropDetails: drop contained 1:1 mixture of protein and reservoir solution | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Jan 15, 1998 |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→50 Å / Num. obs: 8328 / % possible obs: 89 % / Redundancy: 2.1 % / Biso Wilson estimate: 49.2 Å2 / Rsym value: 0.028 / Net I/σ(I): 16.8 |
Reflection shell | Resolution: 2.5→2.64 Å / Redundancy: 1.2 % / Mean I/σ(I) obs: 6.6 / Rsym value: 0.11 / % possible all: 53 |
Reflection | *PLUS Rmerge(I) obs: 0.028 |
Reflection shell | *PLUS % possible obs: 53 % / Rmerge(I) obs: 0.11 |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS / Resolution: 2.5→50 Å / SU B: 3.64 / Cross valid method: THROUGHOUT / σ(F): 0 Details: DISORDERED REGIONS ARE FROM GLY22-GLY28, GLY65-GLU68 AND ILE147-PRO147 WERE MODELED STEREOCHEMICALLY
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Displacement parameters | Biso mean: 60.4 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→50 Å
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Refine LS restraints |
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