[English] 日本語
Yorodumi- PDB-1bhi: STRUCTURE OF TRANSACTIVATION DOMAIN OF CRE-BP1/ATF-2, NMR, 20 STR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1bhi | ||||||
---|---|---|---|---|---|---|---|
Title | STRUCTURE OF TRANSACTIVATION DOMAIN OF CRE-BP1/ATF-2, NMR, 20 STRUCTURES | ||||||
Components | CRE-BP1 | ||||||
Keywords | DNA-BINDING REGULATORY PROTEIN / CRE BINDING PROTEIN / ATF-2 / TRANSCRIPTIONAL ACTIVATION DOMAIN / ZN FINGER | ||||||
Function / homology | Function and homology information abducens nucleus development / hypoglossal nucleus development / detection of cell density / H4 histone acetyltransferase complex / growth plate cartilage chondrocyte proliferation / facial nucleus development / growth plate cartilage chondrocyte differentiation / positive regulation of cardiac muscle myoblast proliferation / cellular response to anisomycin / positive regulation of transforming growth factor beta2 production ...abducens nucleus development / hypoglossal nucleus development / detection of cell density / H4 histone acetyltransferase complex / growth plate cartilage chondrocyte proliferation / facial nucleus development / growth plate cartilage chondrocyte differentiation / positive regulation of cardiac muscle myoblast proliferation / cellular response to anisomycin / positive regulation of transforming growth factor beta2 production / cAMP response element binding / cellular lipid metabolic process / leucine zipper domain binding / cAMP response element binding protein binding / positive regulation of mitochondrial membrane permeability involved in apoptotic process / histone H2B acetyltransferase activity / cellular response to leucine starvation / brainstem development / histone H4 acetyltransferase activity / NK T cell differentiation / vacuole organization / neurofilament cytoskeleton organization / apoptotic process involved in development / NGF-stimulated transcription / intrinsic apoptotic signaling pathway in response to hypoxia / mitotic intra-S DNA damage checkpoint signaling / motor neuron apoptotic process / response to osmotic stress / hepatocyte apoptotic process / outflow tract morphogenesis / Activation of the AP-1 family of transcription factors / p38MAPK cascade / Response of EIF2AK4 (GCN2) to amino acid deficiency / white fat cell differentiation / BMP signaling pathway / adipose tissue development / cis-regulatory region sequence-specific DNA binding / hematopoietic progenitor cell differentiation / histone acetyltransferase activity / JNK cascade / transcription initiation-coupled chromatin remodeling / negative regulation of angiogenesis / liver development / Regulation of PTEN gene transcription / promoter-specific chromatin binding / TP53 Regulates Transcription of DNA Repair Genes / peptidyl-threonine phosphorylation / mRNA transcription by RNA polymerase II / Heme signaling / RNA polymerase II transcription regulatory region sequence-specific DNA binding / Transcriptional activation of mitochondrial biogenesis / response to organic cyclic compound / cellular response to virus / protein import into nucleus / RNA polymerase II transcription regulator complex / positive regulation of DNA-binding transcription factor activity / Circadian Clock / sequence-specific double-stranded DNA binding / cellular response to oxidative stress / site of double-strand break / HATs acetylate histones / DNA-binding transcription activator activity, RNA polymerase II-specific / in utero embryonic development / Estrogen-dependent gene expression / mitochondrial outer membrane / RNA polymerase II-specific DNA-binding transcription factor binding / DNA-binding transcription factor activity, RNA polymerase II-specific / positive regulation of protein phosphorylation / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein heterodimerization activity / DNA-binding transcription factor activity / DNA damage response / chromatin / positive regulation of gene expression / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / protein kinase binding / negative regulation of transcription by RNA polymerase II / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / nucleoplasm / metal ion binding / nucleus / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING, ENERGY MINIMIZATION | ||||||
Authors | Nagadoi, A. / Nakazawa, K. / Uda, H. / Maekawa, T. / Ishii, S. / Nishimura, Y. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1999 Title: Solution structure of the transactivation domain of ATF-2 comprising a zinc finger-like subdomain and a flexible subdomain. Authors: Nagadoi, A. / Nakazawa, K. / Uda, H. / Okuno, K. / Maekawa, T. / Ishii, S. / Nishimura, Y. #1: Journal: Genes Dev. / Year: 1990 Title: Erratum. Transcription Factor ATF Cdna Clones: An Extensive Family of Leucine Zipper Proteins Able to Selectively Form DNA-Binding Heterodimers Authors: Hai, T.W. / Liu, F. / Coukos, W.J. / Green, M.R. #2: Journal: Genes Dev. / Year: 1989 Title: Transcription Factor ATF Cdna Clones: An Extensive Family of Leucine Zipper Proteins Able to Selectively Form DNA-Binding Heterodimers Authors: Hai, T.W. / Liu, F. / Coukos, W.J. / Green, M.R. #3: Journal: Embo J. / Year: 1989 Title: Leucine Zipper Structure of the Protein Cre-BP1 Binding to the Cyclic AMP Response Element in Brain Authors: Maekawa, T. / Sakura, H. / Kanei-Ishii, C. / Sudo, T. / Yoshimura, T. / Fujisawa, J. / Yoshida, M. / Ishii, S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1bhi.cif.gz | 258.3 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1bhi.ent.gz | 226.1 KB | Display | PDB format |
PDBx/mmJSON format | 1bhi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bh/1bhi ftp://data.pdbj.org/pub/pdb/validation_reports/bh/1bhi | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 4336.971 Da / Num. of mol.: 1 Fragment: ZINC FINGER-LIKE SUBDOMAIN OF THE TRANSACTIVATION DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CDNA / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P15336 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|---|
NMR details | Text: THE STRUCTURES OF N- AND C-TERMINI OF THE PEPTIDE FRAGMENT (MET 1 - PRO 6 AND LYS 36 - GLY 38) WERE DISORDERED AMONG THE 20 STRUCTURES. |
-Sample preparation
Sample conditions | pH: 6.3 / Temperature: 300 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX 600 / Manufacturer: Bruker / Model: DMX 600 / Field strength: 600 MHz |
---|
-Processing
NMR software |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: SIMULATED ANNEALING, ENERGY MINIMIZATION / Software ordinal: 1 | |||||||||
NMR ensemble | Conformer selection criteria: THE 20 FINAL STRUCTURES EXHIBITED NO DISTANCE RESTRAINT VIOLATIONS GREATER THAN 0.1 ANGSTROMS AND DIHEDRAL ANGLE VIOLATIONS GREATER THAN 6.0 DEGREES. Conformers calculated total number: 100 / Conformers submitted total number: 20 |