+Open data
-Basic information
Entry | Database: PDB / ID: 1b38 | ||||||
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Title | HUMAN CYCLIN-DEPENDENT KINASE 2 | ||||||
Components | PROTEIN (CELL DIVISION PROTEIN KINASE 2) | ||||||
Keywords | TRANSFERASE / PROTEIN KINASE / SERINE/THREONINE PROTEIN KINASE / ATP-BINDING / CELL CYCLE / CELL DIVISION / MITOSIS / PHOSPHORYLATION | ||||||
Function / homology | Function and homology information Replication initiator protein RctB, central region / RctB, helix turn helix domain / Vibrionales, replication initiator protein RctB, central region / RctB helix turn helix domain / Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Protein kinase domain / 2-Layer Sandwich ...Replication initiator protein RctB, central region / RctB, helix turn helix domain / Vibrionales, replication initiator protein RctB, central region / RctB helix turn helix domain / Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Protein kinase domain / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2 Å | ||||||
Authors | Brown, N.R. / Noble, M.E.M. / Lawrie, A.M. / Morris, M.C. / Tunnah, P. / Divita, G. / Johnson, L.N. / Endicott, J.A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1999 Title: Effects of phosphorylation of threonine 160 on cyclin-dependent kinase 2 structure and activity. Authors: Brown, N.R. / Noble, M.E. / Lawrie, A.M. / Morris, M.C. / Tunnah, P. / Divita, G. / Johnson, L.N. / Endicott, J.A. #1: Journal: Proteins / Year: 1995 Title: Multiple Modes of Ligand Recognition: Crystal Structures of Cyclin-Dependent Protein Kinase 2 in Complex with ATP and Two Inhibitors, Olomoucine and Isopentenyladenine Authors: Schulze-Gahmen, U. / Brandsen, J. / Jones, H.D. / Morgan, D.O. / Meijer, L. / Vesely, J. / Kim, S.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b38.cif.gz | 78.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b38.ent.gz | 57.2 KB | Display | PDB format |
PDBx/mmJSON format | 1b38.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1b38_validation.pdf.gz | 763.5 KB | Display | wwPDB validaton report |
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Full document | 1b38_full_validation.pdf.gz | 775.1 KB | Display | |
Data in XML | 1b38_validation.xml.gz | 16.8 KB | Display | |
Data in CIF | 1b38_validation.cif.gz | 24.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b3/1b38 ftp://data.pdbj.org/pub/pdb/validation_reports/b3/1b38 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34002.527 Da / Num. of mol.: 1 / Fragment: INTACT Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: BACULOVIRUS / Cell line (production host): SF9 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P24941, EC: 2.7.1.37 |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-ATP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.01 Å3/Da / Density % sol: 45 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.4 Details: PROTEIN AT 10 MG/ML IN 10MM HEPES/HCL PH7.4, 15MM NACL WELL BUFFER CONTAINING 50MM AMMONIUM ACETATE, 12% PEG 3350, 100MM HEPES/HCL PH 7.4 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.5 / Wavelength: 1.5418 |
Detector | Type: MAR scanner 300 mm plate / Detector: IMAGE PLATE / Date: May 21, 1998 / Details: MIRROR |
Radiation | Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 18076 / % possible obs: 95.2 % / Observed criterion σ(I): 0 / Redundancy: 3.9 % / Rmerge(I) obs: 0.092 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2→2.1 Å / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 2.26 / % possible all: 96.8 |
Reflection | *PLUS Num. measured all: 70729 |
Reflection shell | *PLUS % possible obs: 96.8 % |
-Processing
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Refinement | Method to determine structure: OTHER Starting model: UNPUBLISHED Resolution: 2→20 Å / Cross valid method: FREE-R / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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