+Open data
-Basic information
Entry | Database: PDB / ID: 1au1 | ||||||||||||
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Title | HUMAN INTERFERON-BETA CRYSTAL STRUCTURE | ||||||||||||
Components | INTERFERON-BETA | ||||||||||||
Keywords | INTERFERON / HELICAL CYTOKINE / IMMUNE SYSTEM / CYTOKINE | ||||||||||||
Function / homology | Function and homology information regulation of MHC class I biosynthetic process / B cell activation involved in immune response / type I interferon receptor binding / chloramphenicol O-acetyltransferase activity / negative regulation of T cell differentiation / natural killer cell activation involved in immune response / positive regulation of peptidyl-serine phosphorylation of STAT protein / negative regulation of T-helper 2 cell cytokine production / T cell activation involved in immune response / natural killer cell activation ...regulation of MHC class I biosynthetic process / B cell activation involved in immune response / type I interferon receptor binding / chloramphenicol O-acetyltransferase activity / negative regulation of T cell differentiation / natural killer cell activation involved in immune response / positive regulation of peptidyl-serine phosphorylation of STAT protein / negative regulation of T-helper 2 cell cytokine production / T cell activation involved in immune response / natural killer cell activation / positive regulation of innate immune response / TRAF6 mediated IRF7 activation / negative regulation of viral genome replication / response to exogenous dsRNA / cellular response to exogenous dsRNA / type I interferon-mediated signaling pathway / B cell proliferation / humoral immune response / Regulation of IFNA/IFNB signaling / cellular response to interferon-beta / positive regulation of apoptotic signaling pathway / B cell differentiation / cytokine activity / response to virus / cytokine-mediated signaling pathway / Interferon alpha/beta signaling / blood coagulation / TRAF3-dependent IRF activation pathway / Factors involved in megakaryocyte development and platelet production / defense response to virus / Oxidative Stress Induced Senescence / adaptive immune response / cell surface receptor signaling pathway / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||||||||
Authors | Karpusas, M. / Nolte, M. / Lipscomb, W. | ||||||||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1997 Title: The crystal structure of human interferon beta at 2.2-A resolution. Authors: Karpusas, M. / Nolte, M. / Benton, C.B. / Meier, W. / Lipscomb, W.N. / Goelz, S. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1au1.cif.gz | 98.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1au1.ent.gz | 80.8 KB | Display | PDB format |
PDBx/mmJSON format | 1au1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/au/1au1 ftp://data.pdbj.org/pub/pdb/validation_reports/au/1au1 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 20054.080 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): CHO / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: P01574 #2: Polysaccharide | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-3)-beta-D- ...beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-[alpha-D-quinovopyranose-(1-6)]beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #3: Polysaccharide | alpha-D-quinovopyranose-(1-6)-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #4: Chemical | ChemComp-ZN / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.83 Å3/Da / Density % sol: 55.43 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5 / Method: vapor diffusion, hanging dropDetails: drop solution was mixed with an equal volume of reservoir solution | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 108 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jan 10, 1996 / Details: MIRRORS |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→30 Å / Num. obs: 18405 / % possible obs: 83.1 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.0766 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.2→30 Å / Cross valid method: THROUGHOUT / σ(F): 2 Details: RESIDUES 28 - 30 OF MOLECULE B HAVE NOT BEEN MODELED DUE TO WEAK ELECTRON DENSITY.
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Refinement step | Cycle: LAST / Resolution: 2.2→30 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.81 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |