+Open data
-Basic information
Entry | Database: PDB / ID: 1aec | ||||||
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Title | CRYSTAL STRUCTURE OF ACTINIDIN-E-64 COMPLEX+ | ||||||
Components | ACTINIDIN | ||||||
Keywords | HYDROLASE | ||||||
Function / homology | Function and homology information actinidain / proteolysis involved in protein catabolic process / lysosome / cysteine-type endopeptidase activity / extracellular space Similarity search - Function | ||||||
Biological species | Actinidia chinensis (golden kiwifruit) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.86 Å | ||||||
Authors | Varughese, K.I. | ||||||
Citation | Journal: Biochemistry / Year: 1992 Title: Crystal structure of an actinidin-E-64 complex. Authors: Varughese, K.I. / Su, Y. / Cromwell, D. / Hasnain, S. / Xuong, N.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1aec.cif.gz | 67.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1aec.ent.gz | 48.9 KB | Display | PDB format |
PDBx/mmJSON format | 1aec.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1aec_validation.pdf.gz | 428.1 KB | Display | wwPDB validaton report |
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Full document | 1aec_full_validation.pdf.gz | 430.1 KB | Display | |
Data in XML | 1aec_validation.xml.gz | 6.3 KB | Display | |
Data in CIF | 1aec_validation.cif.gz | 10.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ae/1aec ftp://data.pdbj.org/pub/pdb/validation_reports/ae/1aec | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 155 2: THE SIDE CHAIN OF SER 9 IS MODELLED WITH AN ALTERNATE CONFORMATION DUE TO DISORDER. |
-Components
#1: Protein | Mass: 23569.971 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Actinidia chinensis (golden kiwifruit) / References: UniProt: P00785, actinidain |
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#2: Chemical | ChemComp-E64 / |
#3: Water | ChemComp-HOH / |
Sequence details | SEQUENCE ADVISORY NOTICE: DIFFERENCE BETWEEN PIR AND PDB SEQUENCE. PIR ENTRY NAME: PIR:S12618 ...SEQUENCE ADVISORY NOTICE: DIFFERENCE |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.13 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.86 Å / Num. obs: 96911 / % possible obs: 94 % / Num. measured all: 17408 |
-Processing
Software |
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Refinement | Resolution: 1.86→8 Å / Rfactor Rwork: 0.145 / Rfactor obs: 0.145 / σ(I): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.86→8 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.86 Å / Lowest resolution: 8 Å / Num. reflection obs: 17116 / σ(I): 2 / Rfactor obs: 0.145 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d |