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Yorodumi- PDB-1a0n: NMR STUDY OF THE SH3 DOMAIN FROM FYN PROTO-ONCOGENE TYROSINE KINA... -
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-Basic information
Entry | Database: PDB / ID: 1a0n | ||||||
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Title | NMR STUDY OF THE SH3 DOMAIN FROM FYN PROTO-ONCOGENE TYROSINE KINASE COMPLEXED WITH THE SYNTHETIC PEPTIDE P2L CORRESPONDING TO RESIDUES 91-104 OF THE P85 SUBUNIT OF PI3-KINASE, FAMILY OF 25 STRUCTURES | ||||||
Components |
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Keywords | COMPLEX (PHOSPHOTRANSFERASE/PEPTIDE) / COMPLEX (PHOSPHOTRANSFERASE-PEPTIDE) / SH3 DOMAIN / POLYPROLINE-BINDING / COMPLEX (PHOSPHOTRANSFERASE-PEPTIDE) complex | ||||||
Function / homology | Function and homology information response to singlet oxygen / Reelin signalling pathway / negative regulation of hydrogen peroxide biosynthetic process / perinuclear endoplasmic reticulum / perinuclear endoplasmic reticulum membrane / regulation of toll-like receptor 4 signaling pathway / NTRK2 activates RAC1 / Activated NTRK2 signals through FYN / heart process / phosphatidylinositol kinase activity ...response to singlet oxygen / Reelin signalling pathway / negative regulation of hydrogen peroxide biosynthetic process / perinuclear endoplasmic reticulum / perinuclear endoplasmic reticulum membrane / regulation of toll-like receptor 4 signaling pathway / NTRK2 activates RAC1 / Activated NTRK2 signals through FYN / heart process / phosphatidylinositol kinase activity / cellular response to L-glutamate / growth factor receptor binding / phosphatidylinositol 3-kinase regulator activity / IRS-mediated signalling / positive regulation of focal adhesion disassembly / phosphatidylinositol 3-kinase activator activity / regulation of glutamate receptor signaling pathway / regulation of calcium ion import across plasma membrane / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / interleukin-18-mediated signaling pathway / PI3K events in ERBB4 signaling / myeloid leukocyte migration / 1-phosphatidylinositol-3-kinase regulator activity / Platelet Adhesion to exposed collagen / CD28 co-stimulation / G protein-coupled glutamate receptor signaling pathway / phosphatidylinositol 3-kinase regulatory subunit binding / neurotrophin TRKA receptor binding / Activated NTRK2 signals through PI3K / positive regulation of protein localization to membrane / activated T cell proliferation / CRMPs in Sema3A signaling / Activated NTRK3 signals through PI3K / cis-Golgi network / ErbB-3 class receptor binding / kinase activator activity / positive regulation of cysteine-type endopeptidase activity / FLT3 signaling through SRC family kinases / transmembrane receptor protein tyrosine kinase adaptor activity / RHOF GTPase cycle / RHOD GTPase cycle / Signaling by cytosolic FGFR1 fusion mutants / positive regulation of endoplasmic reticulum unfolded protein response / negative regulation of dendritic spine maintenance / feeding behavior / type 5 metabotropic glutamate receptor binding / Nef and signal transduction / phosphatidylinositol 3-kinase complex, class IA / enzyme-substrate adaptor activity / phosphatidylinositol 3-kinase complex / Nephrin family interactions / Signaling by LTK in cancer / DCC mediated attractive signaling / PI3K events in ERBB2 signaling / EPH-Ephrin signaling / dendrite morphogenesis / RND1 GTPase cycle / Costimulation by the CD28 family / Signaling by LTK / CD28 dependent Vav1 pathway / Ephrin signaling / positive regulation of leukocyte migration / MET activates PI3K/AKT signaling / RND2 GTPase cycle / PI3K/AKT activation / positive regulation of filopodium assembly / RND3 GTPase cycle / negative regulation of stress fiber assembly / growth hormone receptor signaling pathway / dendritic spine maintenance / natural killer cell mediated cytotoxicity / insulin binding / RHOV GTPase cycle / Regulation of KIT signaling / Signaling by ALK / negative regulation of cell-matrix adhesion / RHOB GTPase cycle / tau-protein kinase activity / GP1b-IX-V activation signalling / CTLA4 inhibitory signaling / phospholipase activator activity / leukocyte migration / GAB1 signalosome / PI-3K cascade:FGFR3 / Erythropoietin activates Phosphoinositide-3-kinase (PI3K) / Fc-gamma receptor signaling pathway involved in phagocytosis / EPHA-mediated growth cone collapse / cellular response to platelet-derived growth factor stimulus / PI-3K cascade:FGFR2 / Dectin-2 family / PI-3K cascade:FGFR4 / RHOC GTPase cycle / RHOJ GTPase cycle / PI-3K cascade:FGFR1 / negative regulation of osteoclast differentiation / intracellular glucose homeostasis / stimulatory C-type lectin receptor signaling pathway / PECAM1 interactions / regulation of peptidyl-tyrosine phosphorylation / CD28 dependent PI3K/Akt signaling Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Renzoni, D.A. / Pugh, D.J.R. / Siligardi, G. / Das, P. / Morton, C.J. / Rossi, C. / Waterfield, M.D. / Campbell, I.D. / Ladbury, J.E. | ||||||
Citation | Journal: Biochemistry / Year: 1996 Title: Structural and thermodynamic characterization of the interaction of the SH3 domain from Fyn with the proline-rich binding site on the p85 subunit of PI3-kinase. Authors: Renzoni, D.A. / Pugh, D.J. / Siligardi, G. / Das, P. / Morton, C.J. / Rossi, C. / Waterfield, M.D. / Campbell, I.D. / Ladbury, J.E. #1: Journal: Structure / Year: 1996 Title: Solution Structure and Peptide Binding of the SH3 Domain from Human Fyn Authors: Morton, C.J. / Pugh, D.J. / Brown, E.L. / Kahmann, J.D. / Renzoni, D.A. / Campbell, I.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
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PDBx/mmCIF format | 1a0n.cif.gz | 544 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a0n.ent.gz | 453.9 KB | Display | PDB format |
PDBx/mmJSON format | 1a0n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1a0n_validation.pdf.gz | 353.4 KB | Display | wwPDB validaton report |
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Full document | 1a0n_full_validation.pdf.gz | 679.4 KB | Display | |
Data in XML | 1a0n_validation.xml.gz | 39.1 KB | Display | |
Data in CIF | 1a0n_validation.cif.gz | 60.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a0/1a0n ftp://data.pdbj.org/pub/pdb/validation_reports/a0/1a0n | HTTPS FTP |
-Related structure data
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-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1405.640 Da / Num. of mol.: 1 / Fragment: P85 SUBUNIT OF PI3-KINASE, RESIDUES 91 - 104 Source method: isolated from a genetically manipulated source References: UniProt: P27986 |
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#2: Protein | Mass: 7698.238 Da / Num. of mol.: 1 / Fragment: SH3 DOMAIN, RESIDUES 82 - 148 / Mutation: N-TERMINAL GS FROM EXPRESSION SYSTEM Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PGEX / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) / References: UniProt: P06241, EC: 2.7.1.112 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Crystal grow | *PLUS Method: other / Details: NMR |
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-Processing
Software |
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NMR software | Name: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement | ||||||||||||
NMR ensemble | Conformers calculated total number: 50 / Conformers submitted total number: 25 |