+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8bfg | ||||||||||||||||||
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タイトル | Solution structure of human apo/Calmodulin G113R (G114R) | ||||||||||||||||||
要素 | Calmodulin-1カルモジュリン | ||||||||||||||||||
キーワード | METAL BINDING PROTEIN / Calcium-binding protein Calcium signalling Cardiac arrhythmia | ||||||||||||||||||
機能・相同性 | 機能・相同性情報 CAM型光合成 / Cam-PDE 1 activation / Sodium/Calcium exchangers / Calmodulin induced events / Reduction of cytosolic Ca++ levels / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / Activation of Ca-permeable Kainate Receptor / Loss of phosphorylation of MECP2 at T308 / CREB1 phosphorylation through the activation of Adenylate Cyclase / PKA activation ...CAM型光合成 / Cam-PDE 1 activation / Sodium/Calcium exchangers / Calmodulin induced events / Reduction of cytosolic Ca++ levels / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / Activation of Ca-permeable Kainate Receptor / Loss of phosphorylation of MECP2 at T308 / CREB1 phosphorylation through the activation of Adenylate Cyclase / PKA activation / negative regulation of high voltage-gated calcium channel activity / CaMK IV-mediated phosphorylation of CREB / Glycogen breakdown (glycogenolysis) / organelle localization by membrane tethering / negative regulation of calcium ion export across plasma membrane / Activation of RAC1 downstream of NMDARs / mitochondrion-endoplasmic reticulum membrane tethering / CLEC7A (Dectin-1) induces NFAT activation / regulation of cardiac muscle cell action potential / autophagosome membrane docking / positive regulation of ryanodine-sensitive calcium-release channel activity / Negative regulation of NMDA receptor-mediated neuronal transmission / regulation of cell communication by electrical coupling involved in cardiac conduction / negative regulation of peptidyl-threonine phosphorylation / Unblocking of NMDA receptors, glutamate binding and activation / Synthesis of IP3 and IP4 in the cytosol / Phase 0 - rapid depolarisation / protein phosphatase activator activity / RHO GTPases activate PAKs / positive regulation of cyclic-nucleotide phosphodiesterase activity / positive regulation of phosphoprotein phosphatase activity / Ion transport by P-type ATPases / 長期増強 / Uptake and function of anthrax toxins / Calcineurin activates NFAT / Regulation of MECP2 expression and activity / catalytic complex / DARPP-32 events / detection of calcium ion / negative regulation of ryanodine-sensitive calcium-release channel activity / Smooth Muscle Contraction / RHO GTPases activate IQGAPs / regulation of cardiac muscle contraction / calcium channel inhibitor activity / cellular response to interferon-beta / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / Protein methylation / voltage-gated potassium channel complex / eNOS activation / Activation of AMPK downstream of NMDARs / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / regulation of calcium-mediated signaling / positive regulation of protein dephosphorylation / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / titin binding / Ion homeostasis / regulation of ryanodine-sensitive calcium-release channel activity / positive regulation of protein autophosphorylation / sperm midpiece / calcium channel complex / substantia nigra development / adenylate cyclase activator activity / Ras activation upon Ca2+ influx through NMDA receptor / regulation of heart rate / protein serine/threonine kinase activator activity / sarcomere / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / VEGFR2 mediated vascular permeability / positive regulation of peptidyl-threonine phosphorylation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / VEGFR2 mediated cell proliferation / regulation of cytokinesis / Translocation of SLC2A4 (GLUT4) to the plasma membrane / spindle microtubule / RAF activation / positive regulation of receptor signaling pathway via JAK-STAT / positive regulation of protein serine/threonine kinase activity / Transcriptional activation of mitochondrial biogenesis / Stimuli-sensing channels / 紡錘体 / cellular response to type II interferon / response to calcium ion / RAS processing / calcium-dependent protein binding / Inactivation, recovery and regulation of the phototransduction cascade / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / G2/M transition of mitotic cell cycle / Signaling by BRAF and RAF1 fusions / Platelet degranulation / 髄鞘 / Ca2+ pathway / RAF/MAP kinase cascade / vesicle / transmembrane transporter binding / Extra-nuclear estrogen signaling / G protein-coupled receptor signaling pathway 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | Homo sapiens (ヒト) | ||||||||||||||||||
手法 | 溶液NMR / simulated annealing | ||||||||||||||||||
データ登録者 | Wimmer, R. / Holler, C.V. / Petersson, N.M. / Brohus, M.B. / Niemelae, M. / Overgaard, M.T. / Iwai, H. | ||||||||||||||||||
資金援助 | デンマーク, 5件
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引用 | ジャーナル: Cell Calcium / 年: 2023 タイトル: Allosteric changes in protein stability and dynamics as pathogenic mechanism for calmodulin variants not affecting Ca 2+ coordinating residues. 著者: Holler, C.V. / Petersson, N.M. / Brohus, M. / Niemela, M.A. / Iversen, E.D. / Overgaard, M.T. / Iwai, H. / Wimmer, R. | ||||||||||||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8bfg.cif.gz | 425.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8bfg.ent.gz | 347.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8bfg.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/bf/8bfg ftp://data.pdbj.org/pub/pdb/validation_reports/bf/8bfg | HTTPS FTP |
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-関連構造データ
関連構造データ | 8bd2C C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
その他のデータベース |
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-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 16821.492 Da / 分子数: 1 / 変異: G113R / 由来タイプ: 組換発現 詳細: The sample contained native calmodulin, that was segmentally isotope labelled with 13C and 15N from amino acids 81-148, while amino acids 1-80 were unlabelled. Thus, even though the whole ...詳細: The sample contained native calmodulin, that was segmentally isotope labelled with 13C and 15N from amino acids 81-148, while amino acids 1-80 were unlabelled. Thus, even though the whole native calmodulin sequence was present in the sample, only the structure of amino acids 81-148 was determined. 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: CALM1, CALM, CAM, CAM1 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0DP23 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | タイプ: solution 内容: 1.2 mM aa 81-148: U-13C,15N Calmodulin G113R, 5 mM EDTA, 100 mM potassium chloride, 2 mM NaN3, 20 mM HEPES, 0.1 mM TSP-d4, 95% H2O/5% D2O 詳細: The sample contained 1.2 mM apo/CaM-G113R, 5 mM EDTA, 100 mM KCl, 5mM NaN3, 0.1 mM TSP-d4 dissolved in 20 mM HEPES. Label: sample1 / 溶媒系: 95% H2O/5% D2O | ||||||||||||||||||||||||||||
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試料 |
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試料状態 | イオン強度: 128 mM / Label: sample1 / pH: 6.64 pH* / 圧: 1 atm / 温度: 298.1 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker AVANCE III / 製造業者: Bruker / モデル: AVANCE III / 磁場強度: 600 MHz / 詳細: CPP-TCI probe |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: two steps: 1) in vacuo with the NOVA force field, 2) in explicit water with the YASARA force field | ||||||||||||||||||||
代表構造 | 選択基準: target function | ||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20 |