Component-ID: 0 / Ens-ID: 1 / Beg auth comp-ID: PRO / Beg label comp-ID: PRO / End auth comp-ID: LYS / End label comp-ID: LYS / Refine code: 0 / Auth seq-ID: 8 - 369 / Label seq-ID: 26 - 387
Dom-ID
Auth asym-ID
Label asym-ID
1
A
A
2
B
B
-
要素
#1: タンパク質
heteroyohimbinesynthaseTHAS2
分子量: 42618.859 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: Native N-terminal MET is replaced by an affinity tag with the sequence MAHHHHHHSSGLEVLFQGP Ignore Uniprot information below 由来: (組換発現) Catharanthus roseus (ニチニチカ) 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / Variant (発現宿主): SOLUBL21 / 参照: UniProt: A0A1C7D193*PLUS
解像度: 2.05→60.69 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.911 / WRfactor Rfree: 0.1992 / WRfactor Rwork: 0.1686 / FOM work R set: 0.8345 / SU B: 8.66 / SU ML: 0.122 / SU R Cruickshank DPI: 0.1815 / SU Rfree: 0.1606 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.182 / ESU R Free: 0.161 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
Rfactor
反射数
%反射
Selection details
Rfree
0.2254
2516
5.1 %
RANDOM
Rwork
0.1862
-
-
-
obs
0.1882
46829
99.87 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK