+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 3w80 | ||||||
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タイトル | Crystal structure of dodecamer human insulin with double C-axis length of the hexamer 2 Zn insulin cell | ||||||
要素 | (Insulinインスリン) x 2 | ||||||
キーワード | HORMONE (ホルモン) / Human insulin (インスリン製剤) / glucose metabolism (炭水化物代謝) / secretion (分泌) | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion / positive regulation of peptide hormone secretion / Regulation of gene expression in beta cells / positive regulation of respiratory burst / positive regulation of dendritic spine maintenance / alpha-beta T cell activation / negative regulation of acute inflammatory response / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / fatty acid homeostasis / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of glycogen biosynthetic process / positive regulation of lipid biosynthetic process / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of gluconeogenesis / positive regulation of nitric oxide mediated signal transduction / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / negative regulation of lipid catabolic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / negative regulation of reactive oxygen species biosynthetic process / positive regulation of insulin receptor signaling pathway / 小胞 / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of brown fat cell differentiation / activation of protein kinase B activity / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of mitotic nuclear division / Regulation of insulin secretion / positive regulation of nitric-oxide synthase activity / positive regulation of long-term synaptic potentiation / endosome lumen / positive regulation of cytokine production / acute-phase response / positive regulation of protein secretion / regulation of transmembrane transporter activity / positive regulation of cell differentiation / positive regulation of glucose import / negative regulation of proteolysis / regulation of synaptic plasticity / wound healing / insulin receptor binding / negative regulation of protein catabolic process / positive regulation of neuron projection development / hormone activity / 認識 / Golgi lumen / vasodilation / positive regulation of protein localization to nucleus / glucose metabolic process / regulation of protein localization / glucose homeostasis / cell-cell signaling / insulin receptor signaling pathway / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / 小胞体 / ゴルジ体 / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 1.4 Å | ||||||
データ登録者 | Murayoshi, M. / Sasaki, K. / Sakabe, N. / Sakabe, K. | ||||||
引用 | ジャーナル: To be Published タイトル: Crystal structure of dodecamer insulin with double C-axis length of the hexamer 2 Zn insulin celll 著者: Murayoshi, M. / Sasaki, K. / Sakabe, N. / Sakabe, K. #1: ジャーナル: NUCL INSTRUM METHODS PHYS RES A / 年: 2001 タイトル: Automatic Weissenberg data collection system for time-resolved protein crystallography 著者: Sakabe, N. / Sakabe, K. / Higashi, T. / Igarashi, N. / Suzuki, M. / Watanabe, N. / Sasaki, K. #2: ジャーナル: J.SYNCHROTRON RADIAT. / 年: 1999 タイトル: Rotated-inclined focusing monochromator with simultaneous tuning of asymmetry factor and radius of curvature over a wide wavelength range 著者: Watanabe, N. / Suzuki, M. / Higashi, Y. / Sakabe, N. #3: ジャーナル: J.SYNCHROTRON RADIAT. / 年: 1997 タイトル: Large-Format Imaging Plate and Weissenberg Camera for Accurate Protein Crystallographic Data Collection Using Synchrotron Radiation 著者: Sakabe, K. / Sasaki, K. / Watanabe, N. / Suzuki, M. / Wang, Z.G. / Miyahara, J. / Sakabe, N. #4: ジャーナル: PHILOS.TRANS.R.SOC.LOND.B BIOL.SCI. / 年: 1988 タイトル: The structure of 2Zn pig insulin crystals at 1.5A resolution 著者: Baker, E. / Blundell, T.L. / Cutfield, J.F. / Cutfield, S.M. / Dodson, E.J. / Dodson, G. / Hodgkin, D.M.C. / Hubbard, E. / Isaacs, N.W. / Reynolds, C.D. / Sakabe, K. / Sakabe, N. / Vijayan, N.M. #5: ジャーナル: Acta Crystallogr.,Sect.A / 年: 1972 タイトル: A statistical evaluation of absorption 著者: Katayama, C. / Sakabe, N. / Sakabe, K. #6: ジャーナル: J.APPL.CRYSTALLOGR. / 年: 1989 タイトル: The processing of diffraction data taken on a screenless Weissenberg camera for macromolecular crystallography 著者: Higashi, T. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 3w80.cif.gz | 109 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb3w80.ent.gz | 85.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 3w80.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/w8/3w80 ftp://data.pdbj.org/pub/pdb/validation_reports/w8/3w80 | HTTPS FTP |
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-関連構造データ
関連構造データ | 3w7yS S: 精密化の開始モデル |
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類似構造データ |
-リンク
-集合体
登録構造単位 |
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単位格子 |
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Components on special symmetry positions |
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-要素
#1: タンパク質・ペプチド | 分子量: 2383.698 Da / 分子数: 4 / 断片: UNP residues 90-110 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 4 / 断片: UNP residues 25-54 / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: P01308 #3: 化合物 | ChemComp-ZN / #4: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 1.93 Å3/Da / 溶媒含有率: 36.2 % |
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8.67 詳細: 10mg/ml protein, 0.1M Sodium Citrate, 22%(v/v) DMF, 0.08%(w/v) Zinc chloride, pH 8.67, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: シンクロトロン / サイト: Photon Factory / ビームライン: BL-6C / 波長: 1 Å |
検出器 | タイプ: Fully automatic high speed Weissenberg data collection system called Galaxy 検出器: IMAGE PLATE / 日付: 2003年11月5日 詳細: The vertically focusing 1m long bent mirror of Pt-coated fused silica is 21m from the SR source point and 7m from the focus point |
放射 | モノクロメーター: Rotated-inclined focusing Si(111) crystal プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1 Å / 相対比: 1 |
反射 | 解像度: 1→50 Å / Num. obs: 90240 / 冗長度: 4.46 % / Rmerge(I) obs: 0.05476 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: 3w7y 解像度: 1.4→10 Å / Cor.coef. Fo:Fc: 0.985 / Cor.coef. Fo:Fc free: 0.966 / SU B: 3.964 / SU ML: 0.065 / Isotropic thermal model: anisotropic / 交差検証法: THROUGHOUT / ESU R: 0.095 / ESU R Free: 0.087 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 24.619 Å2
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精密化ステップ | サイクル: LAST / 解像度: 1.4→10 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 1.4→1.436 Å / Total num. of bins used: 20
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