+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2kbi | ||||||
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タイトル | Solution NMR structure of the C-terminal EF-hand domain of human cardiac sodium channel NaV1.5 | ||||||
要素 | Sodium channel protein type 5 subunit alphaナトリウムチャネル | ||||||
キーワード | METAL BINDING PROTEIN / protein (タンパク質) / EF-hand (EFハンド) / sodium channel (ナトリウムチャネル) / Brugada syndrome / Cardiomyopathy (心筋症) / Disease mutation / Glycoprotein (糖タンパク質) / Ion transport / Ionic channel (イオンチャネル) / Long QT syndrome / Membrane (生体膜) / Phosphoprotein / Polymorphism / Sodium (ナトリウム) / Sodium transport / Transmembrane (膜貫通型タンパク質) / Transport / Ubl conjugation / Voltage-gated channel (電位依存性イオンチャネル) | ||||||
機能・相同性 | 機能・相同性情報 voltage-gated sodium channel activity involved in AV node cell action potential / voltage-gated sodium channel activity involved in bundle of His cell action potential / voltage-gated sodium channel activity involved in SA node cell action potential / bundle of His cell action potential / AV node cell action potential / SA node cell action potential / AV node cell to bundle of His cell communication / membrane depolarization during SA node cell action potential / cardiac ventricle development / response to denervation involved in regulation of muscle adaptation ...voltage-gated sodium channel activity involved in AV node cell action potential / voltage-gated sodium channel activity involved in bundle of His cell action potential / voltage-gated sodium channel activity involved in SA node cell action potential / bundle of His cell action potential / AV node cell action potential / SA node cell action potential / AV node cell to bundle of His cell communication / membrane depolarization during SA node cell action potential / cardiac ventricle development / response to denervation involved in regulation of muscle adaptation / regulation of ventricular cardiac muscle cell membrane depolarization / regulation of atrial cardiac muscle cell membrane repolarization / membrane depolarization during atrial cardiac muscle cell action potential / membrane depolarization during action potential / voltage-gated sodium channel activity involved in Purkinje myocyte action potential / regulation of sodium ion transmembrane transport / brainstem development / membrane depolarization during AV node cell action potential / voltage-gated sodium channel activity involved in cardiac muscle cell action potential / membrane depolarization during bundle of His cell action potential / positive regulation of action potential / atrial cardiac muscle cell action potential / membrane depolarization during Purkinje myocyte cell action potential / telencephalon development / cardiac conduction system development / regulation of atrial cardiac muscle cell membrane depolarization / voltage-gated sodium channel complex / membrane depolarization during cardiac muscle cell action potential / positive regulation of sodium ion transport / cardiac muscle cell action potential involved in contraction / ventricular cardiac muscle cell action potential / high voltage-gated calcium channel activity / regulation of cardiac muscle cell contraction / voltage-gated sodium channel activity / regulation of ventricular cardiac muscle cell membrane repolarization / Interaction between L1 and Ankyrins / ankyrin binding / sodium ion transport / fibroblast growth factor binding / voltage-gated calcium channel complex / nitric-oxide synthase binding / Phase 0 - rapid depolarisation / odontogenesis of dentin-containing tooth / regulation of heart rate by cardiac conduction / 脱分極 / calcium ion import across plasma membrane / sodium ion transmembrane transport / 介在板 / lateral plasma membrane / cardiac muscle contraction / 横行小管 / cellular response to calcium ion / regulation of heart rate / cerebellum development / positive regulation of epithelial cell proliferation / カベオラ / 筋鞘 / Z disc / scaffold protein binding / transmembrane transporter binding / calmodulin binding / protein domain specific binding / ubiquitin protein ligase binding / 核小体 / protein kinase binding / perinuclear region of cytoplasm / enzyme binding / 細胞膜 / 小胞体 / 核質 / 生体膜 / 細胞膜 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / molecular dynamics | ||||||
データ登録者 | Chagot, B. / Potet, F. / Balser, J.R. / Chazin, W.J. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2009 タイトル: Solution NMR Structure of the C-terminal EF-hand Domain of Human Cardiac Sodium Channel NaV1.5. 著者: Chagot, B. / Potet, F. / Balser, J.R. / Chazin, W.J. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2kbi.cif.gz | 587.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2kbi.ent.gz | 513.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2kbi.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/kb/2kbi ftp://data.pdbj.org/pub/pdb/validation_reports/kb/2kbi | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 10994.207 Da / 分子数: 1 / 断片: EF-Hand domain / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 解説: pET27 derivative / 遺伝子: SCN5A / プラスミド: pBG100 / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): BL21 (DE3) / 参照: UniProt: Q14524 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料 |
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試料状態 | pH: 6.5 / 圧: ambient / 温度: 298 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker AMX / 製造業者: Bruker / モデル: AMX / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: molecular dynamics / ソフトェア番号: 1 | ||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 20 |