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- SASDA62: bAmylase in PBS (Beta-amylase) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDA62
SamplebAmylase in PBS
  • Beta-amylase (protein), Ipomoea batatas
Function / homology
Function and homology information


beta-amylase / beta-amylase activity / amylopectin maltohydrolase activity / polysaccharide catabolic process
Similarity search - Function
Glycoside hydrolase, family 14B, plant / Beta-amylase active site 2. / Glycoside hydrolase, family 14, conserved site / Beta-amylase active site 1. / Glycoside hydrolase, family 14 / Glycosyl hydrolase family 14 / Glycoside hydrolase superfamily
Similarity search - Domain/homology
Biological speciesIpomoea batatas (sweet potato)
Contact author
  • Darja Ruskule (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #8
Type: dummy / Software: dammif / Radius of dummy atoms: 1.90 A / Symmetry: P222 / Chi-square value: 1.234321
Search similar-shape structures of this assembly by Omokage search (details)
Model #7
Type: atomic / Software: crysol (28)
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: bAmylase in PBS / Ext coefficient: 17.68 / Purity method: gel filtration / Sample MW: 200 kDa / Specimen concentration: 12.47 mg/ml / Concentration method: nanodrop
BufferName: PBS / PK: 7 / pH: 7.4
Entity #4Type: protein / Description: Beta-amylase / Formula weight: 56.08 / Num. of mol.: 4 / Source: Ipomoea batatas / References: UniProt: P10537
Sequence: MAPIPGVMPI GNYVSLYVML PLGVVNADNV FPDKEKVEDE LKQVKAGGCD GVMVDVWWGI IEAKGPKQYD WSAYRELFQL VKKCGLKIQA IMSFHQCGGN VGDAVFIPIP QWILQIGDKN PDIFYTNRAG NRNQEYLSLG VDNQRLFQGR TALEMYRDFM ESFRDNMADF ...Sequence:
MAPIPGVMPI GNYVSLYVML PLGVVNADNV FPDKEKVEDE LKQVKAGGCD GVMVDVWWGI IEAKGPKQYD WSAYRELFQL VKKCGLKIQA IMSFHQCGGN VGDAVFIPIP QWILQIGDKN PDIFYTNRAG NRNQEYLSLG VDNQRLFQGR TALEMYRDFM ESFRDNMADF LKAGDIVDIE VGCGAAGELR YPSYPETQGW VFPGIGEFQC YDKYMVADWK EAVKQAGNAD WEMPGKGAGT YNDTPDKTEF FRPNGTYKTD MGKFFLTWYS NKLIIHGDQV LEEANKVFVG LRVNIAAKVS GIHWWYNHVS HAAELTAGFY NVAGRDGYRP IARMLARHHA TLNFTCLEMR DSEQPAEAKS APQELVQQVL SSGWKEYIDV AGENALPRYD ATAYNQMLLN VRPNGVNLNG PPKLKMSGLT YLRLSDDLLQ TDNFELFKKF VKKMHADLDP SPNAISPAVL ERSNSAITID ELMEATKGSR PFPWYDVTDM PVDGSNPFD

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Experimental information

BeamInstrument name: DORIS III X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm
DetectorName: Pilatus 1M-W / Pixsize x: 0.172 mm
Scan
Measurement date: Sep 20, 2012 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 15 sec. / Number of frames: 8 / Unit: 1/nm /
MinMax
Q0.0871 6.0145
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 751 /
MinMax
Q0.221093 2.27436
P(R) point1 751
R0 12.7
Result
Type of curve: merged / Standard: BSA /
ExperimentalStandardPorod
MW162 kDa162 kDa-
Volume--214 nm3

P(R)Guinier
Forward scattering, I0155.7 164.38
Radius of gyration, Rg4.1 nm4.21 nm

MinMax
D-12.7
Guinier point25 85

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