SASDBY3: Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen ...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDBY3

試料

Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state-TBE)

• Aureochrome 1a bZIP-LOV module (protein), PtAUREO1a bZIP-LOV, Phaeodactylum tricornutum

• 生物種: Phaeodactylum tricornutum (海洋性珪藻)
• 引用: ジャーナル: Nucleic Acids Res / 年: 2016; タイトル: Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.; 著者: Ankan Banerjee / Elena Herman / Manuel Serif / Manuel Maestre-Reyna / Sebastian Hepp / Richard Pokorny / Peter G Kroth / Lars-Oliver Essen / Tilman Kottke / ; 要旨: The modular architecture of aureochrome blue light receptors, found in several algal groups including diatoms, is unique by having the LOV-type photoreceptor domain fused to the C-terminus of its putative effector, an N-terminal DNA-binding bZIP module. The structural and functional understanding of aureochromes' light-dependent signaling mechanism is limited, despite their promise as an optogenetic tool. We show that class I aureochromes 1a and 1c from the diatom Phaeodactylum tricornutum are regulated in a light-independent circadian rhythm. These aureochromes are capable to form functional homo- and heterodimers, which recognize the ACGT core sequence within the canonical 'aureo box', TGACGT, in a light-independent manner. The bZIP domain holds a more folded and less flexible but extended conformation in the duplex DNA-bound state. FT-IR spectroscopy in the absence and the presence of DNA shows light-dependent helix unfolding in the LOV domain, which leads to conformational changes in the bZIP region. The solution structure of DNA bound to aureochrome points to a tilted orientation that was further validated by molecular dynamics simulations. We propose that aureochrome signaling relies on an allosteric pathway from LOV to bZIP that results in conformational changes near the bZIP-DNA interface without major effects on the binding affinity.

登録者

• Lars-Oliver Essen (Philipps-Universität Marburg, Marburg, Germany)

モデル

• モデル #459: ; タイプ: dummy / ソフトウェア: DAMFILT / ダミー原子の半径: 2.75 A / コメント: Averaged and filtered/volume corrected model / カイ2乗値: 0.741 / P-value: 0.077000; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state-TBE); 試料濃度: 1.25 mg/ml
• バッファ: 名称: Tris / 濃度: 50.00 mM / pH: 8 / 組成: 50 mM boric acid, 1 mM EDTA

要素 #312

名称: PtAUREO1a bZIP-LOV / タイプ: protein / 記述: Aureochrome 1a bZIP-LOV module / 分子量: 28.655 / 分子数: 2 / 由来: Phaeodactylum tricornutum
配列:

MGSSHHHHHH SSGLVPRGSH MDRKMSEQQK VERRERNREH AKRSRIRKKF LLESLQQSVS LLKEENEKLK TSIRSHLGEE KADTLIDSAN NNKTDVDGLL ASSQGIANKV LDDPDFSFIK ALQTAQQNFV VTDPSLPDNP IVYASQGFLN LTGYSLDQIL GRNCRFLQGP ETDPKAVERI RKAIEQGNDM SVCLLNYRVD GTTFWNQFFI AALRDAGGNV TNFVGVQCKV SDQYAATVTK QQEEEEEAAA NDDED

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.93 Å / スペクトロメータ・検出器間距離: 2.43 mm
• 検出器: 名称: Pilatus 1M

スキャン

タイトル: Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV
測定日: 2014年11月6日 / 保管温度: 4 °C / セル温度: 4 °C / 照射時間: 20 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0364	4.9745

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 337 /

	Min	Max
Q	0.244898	1.83723
P(R) point	1	337
R	0	12.64

結果

カーブのタイプ: single_conc /

	実験値	Porod
分子量	57.5 kDa	57.5 kDa
体積	-	115 nm3

	P(R)	Guinier
前方散乱 I0	57.85	57.85
慣性半径, Rg	3.45 nm	3.41 nm

	Min	Max
D	-	12.64
Guinier point	38	62