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- SASDG84: Autoinhibited dimer of truncated 6xHis Cytohesin-2 (ARNO, amino a... -

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Basic information

Entry
Database: SASBDB / ID: SASDG84
SampleAutoinhibited dimer of truncated 6xHis Cytohesin-2 (ARNO, amino acids 2-400) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF, GASBOR and antiparallel CORAL models)
  • Cytohesin-2 (protein), Cyth2, ARNO, Mus musculus
Function / homology
Function and homology information


Intra-Golgi traffic / inositol 1,4,5 trisphosphate binding / regulation of ARF protein signal transduction / bicellular tight junction / regulation of cell adhesion / guanyl-nucleotide exchange factor activity / adherens junction / growth cone / postsynapse / lipid binding ...Intra-Golgi traffic / inositol 1,4,5 trisphosphate binding / regulation of ARF protein signal transduction / bicellular tight junction / regulation of cell adhesion / guanyl-nucleotide exchange factor activity / adherens junction / growth cone / postsynapse / lipid binding / glutamatergic synapse / plasma membrane / cytoplasm
Similarity search - Function
Sec7 domain / Sec7, C-terminal domain superfamily / Sec7 domain superfamily / Sec7 domain / SEC7 domain profile. / Sec7 domain / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / PH-like domain superfamily
Similarity search - Domain/homology
Biological speciesMus musculus (house mouse)
Contact author
  • David Lambright (UMASS, UMASS Medical School, Worcester, MA, USA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #3648
Type: dummy / Software: (dammif (r9988)) / Radius of dummy atoms: 3.00 A / Symmetry: P1 / Comment: Most representative of 100 DAMMIF models / Chi-square value: 1.219 / P-value: 0.127961
Search similar-shape structures of this assembly by Omokage search (details)
Model #3649
Type: dummy / Software: (DAMFILT 5.0 (r9988)) / Radius of dummy atoms: 4.25 A / Symmetry: P1
Comment: Filtered average of 10 filtered averages of 10 DAMMIF models
Chi-square value: 1.219 / P-value: 0.127961
Search similar-shape structures of this assembly by Omokage search (details)
Model #3682
Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Comment: Most representative of 100 GASBOR models / Chi-square value: 1.30 / P-value: 0.001891
Search similar-shape structures of this assembly by Omokage search (details)
Model #3683
Type: dummy / Software: (DAMFILT 5.0 (r9988)) / Radius of dummy atoms: 3.75 A / Symmetry: P1
Comment: Filtered average of 10 filtered averages of 10 GASBOR models
Chi-square value: 1.30 / P-value: 0.001891
Search similar-shape structures of this assembly by Omokage search (details)
Model #3684
Type: mix / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Derived from Grp1 63-399 (2R09) and a canonical antiparallel coiled coil
Chi-square value: 1.29 / P-value: 0.003841
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Autoinhibited dimer of truncated 6xHis Cytohesin-2 (ARNO, amino acids 2-400) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF, GASBOR and antiparallel CORAL models)
Specimen concentration: 10 mg/ml
BufferName: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate
pH: 8
Entity #1857Name: Cyth2, ARNO / Type: protein / Description: Cytohesin-2 / Formula weight: 47.595 / Num. of mol.: 2 / Source: Mus musculus / References: UniProt: P63034
Sequence: MGHHHHHHGS EDGVYEPPDL TPEERMELEN IRRRKQELLV EIQRLREELS EAMSEVEGLE ANEGSKTLQR NRKMAMGRKK FNMDPKKGIQ FLVEHELLQN TPEEIARFLY KGEGLNKTAI GDYLGEREEL NLSVLHAFVD LHEFTDLNLV QALRQFLWSF RLPGEAQKID ...Sequence:
MGHHHHHHGS EDGVYEPPDL TPEERMELEN IRRRKQELLV EIQRLREELS EAMSEVEGLE ANEGSKTLQR NRKMAMGRKK FNMDPKKGIQ FLVEHELLQN TPEEIARFLY KGEGLNKTAI GDYLGEREEL NLSVLHAFVD LHEFTDLNLV QALRQFLWSF RLPGEAQKID RMMEAFAQRY CLCNPGVFQS TDTCYVLSFA VIMLNTSLHN PNVRDKPGLE RFVAMNRGIN EGGDLPEDLL RNLYDSIRNE PFKIPEDDGN DLTHTFFNPD REGWLLKLGG GRVKTWKRRW FILTDNCLYY FEYTTDKEPR GIIPLENLSI REVDDPRKPN CFELYIPNNK GQLIKACKTE ADGRVVEGNH MVYRISAPTQ EEKDEWIKSI QAAVSVDPFY EMLAARKKRI SVKKKQEQP

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Experimental information

BeamInstrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID
City: Lemont, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.10332 Å / Dist. spec. to detc.: 2.5 mm
DetectorName: MAR 165 CCD
Scan
Title: Autoinhibited dimer of truncated 6xHis Cytohesin-2 (ARNO, amino acids 2-400) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF, GASBOR and antiparallel CORAL models)
Measurement date: Nov 15, 2013 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 121 / Unit: 1/A /
MinMax
Q0.0069 0.3162
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 76 /
MinMax
Q0.00688375 0.316224
P(R) point1 76
R0 270
Result
Type of curve: sec
Comments: The protein was equilibrated with a 1.2 molar excess of inositol 1,3,4,5-tetrakis phosphate (IP4) and concentrated to 10 mg/ml prior to injection.
ExperimentalPorod
MW95 kDa-
Volume-180 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I01.037 0.004 1.03 0.002
Radius of gyration, Rg5.6 nm0.13 5.3 nm0.04

MinMaxError
D-27 0.1
Guinier point1 19 -

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