+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDFS8 |
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試料 | Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)
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機能・相同性 | 機能・相同性情報 methylglyoxal reductase (NADH) / amino acid catabolic process to alcohol via Ehrlich pathway / octanol dehydrogenase (NAD+) activity / methylglyoxal reductase (NADH) activity / glycolytic fermentation to ethanol / butanol dehydrogenase (NAD+) activity / NADH oxidation / melatonin binding / alcohol dehydrogenase (NAD+) activity / alcohol dehydrogenase ...methylglyoxal reductase (NADH) / amino acid catabolic process to alcohol via Ehrlich pathway / octanol dehydrogenase (NAD+) activity / methylglyoxal reductase (NADH) activity / glycolytic fermentation to ethanol / butanol dehydrogenase (NAD+) activity / NADH oxidation / melatonin binding / alcohol dehydrogenase (NAD+) activity / alcohol dehydrogenase / allyl-alcohol dehydrogenase / allyl-alcohol dehydrogenase activity / zinc ion binding / identical protein binding / plasma membrane / cytoplasm 類似検索 - 分子機能 |
生物種 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) |
登録者 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #3521 | タイプ: dummy / ソフトウェア: (SUPCOMB 23 (r9988)) / ダミー原子の半径: 2.10 A / 対称性: P222 / コメント: DAMMIN in P222 prolate symmetry / カイ2乗値: 1.102 / P-value: 0.021141 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #3522 | タイプ: atomic コメント: ADH1, including Zn atoms. 40 harmonics, derived from PDB 4W6Z カイ2乗値: 1.328 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS) 試料濃度: 9.2 mg/ml |
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バッファ | 名称: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, / pH: 7 / コメント: Running buffer for SEC-SAXS |
要素 #1782 | 名称: ADH1 / タイプ: protein / 記述: Alcohol dehydrogenase 1 / 分子量: 36.849 / 分子数: 4 由来: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 参照: UniProt: P00330 配列: MSIPETQKGV IFYESHGKLE YKDIPVPKPK ANELLINVKY SGVCHTDLHA WHGDWPLPVK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA CEYCELGNES NCPHADLSGY THDGSFQQYA TADAVQAAHI PQGTDLAQVA PILCAGITVY KALKSANLMA ...配列: MSIPETQKGV IFYESHGKLE YKDIPVPKPK ANELLINVKY SGVCHTDLHA WHGDWPLPVK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA CEYCELGNES NCPHADLSGY THDGSFQQYA TADAVQAAHI PQGTDLAQVA PILCAGITVY KALKSANLMA GHWVAISGAA GGLGSLAVQY AKAMGYRVLG IDGGEGKEEL FRSIGGEVFI DFTKEKDIVG AVLKATDGGA HGVINVSVSE AAIEASTRYV RANGTTVLVG MPAGAKCCSD VFNQVVKSIS IVGSYVGNRA DTREALDFFA RGLVKSPIKV VGLSTLPEIY EKMEKGQIVG RYVVDTSK |
-実験情報
ビーム | 設備名称: PETRA III EMBL P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron / 波長: 0.123982 Å / スペクトロメータ・検出器間距離: 3 mm | ||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 6M | ||||||||||||||||||||||||||||||
スキャン | タイトル: Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS) 測定日: 2019年4月5日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 67 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 1414 /
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結果 | カーブのタイプ: sec コメント: Alcohol dehydrogenase 1 underwent pre-purification prior to SEC-SAXS using the following method. All procedures were performed at 4 oC. The protein (from Sigma; Gel Filtration Markers ...コメント: Alcohol dehydrogenase 1 underwent pre-purification prior to SEC-SAXS using the following method. All procedures were performed at 4 oC. The protein (from Sigma; Gel Filtration Markers Kit MWGF1000) was made to approximately 25 mg/ml in 25 mM HEPES, 50 mM NaCl, 5 mM urea, 1% v/v glycerol, pH 7. Approximately 200 μl of sample were loaded onto a Superdex 75 Increase 10/300 column (GE Healthcare) equilibrated in the same buffer (flow rate = 0.4 ml/min). Fractionated aliquots corresponding to the highest absorbing peak (estimated using UV A280 and UV A245 nm) were pooled and concentrated (30 kDa centrifuge spin filter) to a final concentration of 9.2 mg/ml (the concentration was determined from triplicate UV A280 measurements using an E0.1% of 1.326 (= 1 g/l) calculated from the amino acid sequence (ProtParam)). Approximately 50 μl aliquots were snap-frozen in liquid nitrogen then stored at -80oC prior to the SEC-SAXS analysis that was performed at room temperature in 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH 7. The Rg-correlation through the SEC-SAXS peak, the individual unsubtracted SEC-SAXS frames as well as the results from coupled MALLS and QELS analysis are included in the full entry zip archive. The quoted experimental molecular weight was determined using MALLS in combination with refractive-index (RI) measurements that were recorded from the same sample eluting from the column using a split-flow SEC-SAXS-light scattering configuration (Graewert et al., (2015) Sci. Reports. 5, 10734: doi: 10.1038/srep10734). The average hydrodynamic radius of the protein is 4.5 nm.
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