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- SASDFJ4: Condensin complex subunit 3-like protein, Ycg1, at 0.5mg/ml -

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Basic information

Entry
Database: SASBDB / ID: SASDFJ4
SampleCondensin complex subunit 3-like protein, Ycg1, at 0.5mg/ml
  • Condensin complex subunit 3-like protein (protein), Ycg1, Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
  • Condensin complex subunit 3-like protein (protein), Ycg1, Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
Function / homologyNuclear condensin complex subunit 3, C-terminal domain / Condensin complex subunit 3 / Nuclear condensing complex subunits, C-term domain / condensin complex / mitotic chromosome condensation / Armadillo-like helical / Armadillo-type fold / cell division / Condensin complex subunit 3-like protein
Function and homology information
Biological speciesChaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Contact author
  • Karen Manalastas-Cantos (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #2914
Type: atomic / Symmetry: P2 / Comment: Dimer is 52% of solution / Chi-square value: 1.30
Search similar-shape structures of this assembly by Omokage search (details)
Model #2915
Type: atomic / Software: (2.8.3) / Symmetry: P1 / Comment: Monomer is 48% of solution / Chi-square value: 1.30
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Condensin complex subunit 3-like protein, Ycg1, at 0.5mg/ml
Specimen concentration: 0.5 mg/ml / Entity id: 1511 / 1566
BufferName: 25 mM Tris 300 mM NaCl 1mM DTT / pH: 7.5
Entity #1511Name: Ycg1 / Type: protein / Description: Condensin complex subunit 3-like protein / Formula weight: 108.325 / Num. of mol.: 1
Source: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
References: UniProt: G0SHK2
Sequence: PDNALRTQVT TVFREAQRTT ASHRKLVVTL RKIQEACCYE PATGQKQSGA AAALSEFDED DFNTEFVRCA LRILPVKKSE GVGEKTVRFV SLFLRHAIEK DNELLGDTQM DEDDADNSTM PETPSTRLTS HLMETILPML MAKDKFVRYR AAQLLAHIVN SLDAIDDDLY ...Sequence:
PDNALRTQVT TVFREAQRTT ASHRKLVVTL RKIQEACCYE PATGQKQSGA AAALSEFDED DFNTEFVRCA LRILPVKKSE GVGEKTVRFV SLFLRHAIEK DNELLGDTQM DEDDADNSTM PETPSTRLTS HLMETILPML MAKDKFVRYR AAQLLAHIVN SLDAIDDDLY QKLRAGLLRR IHDKEPMVRA QAVLGLGRLA GNALDEEPGA GDSDLDGDDD GPGGASGVSL MDKLMDVMKN DPSADVRRTL LVNLPITPKT LPSLLERARD QDPATRRAVY SRLLPALGDF RHLSLSMREK LLRWGLRDRD ENVRKAAARL FRERWIEDCA GTPRPEPGQP IELSPPNMDA LLELLERIDV VNSGGENGIA LEAMRGFWEG RPDYRDAMVF DDHFFETLSA ESVFALRTFN DFCRNEAGGK YEALIEEKLP EVTKLAFYLE RYIKVLIDAI KRAEEQGEEE EEDTAEQEFI VEQLLHIALT LDYADEVGRR KMFALLRQTL SIPELPEECT KLTVMVLRDL CAPDAAGEKE FTSVVLEAVA DVHDTIVDED DDDANVPAED DESFVSARSE VSRESTPAPG GGEGGGSNSR KRGRSPTPKM TEEEAAAKAI REIMINMKCL HIVQCMLQNV QGQLQSNDNL ISMLNNLVVP AVRSHEAPVR ERGLVCLGLC ALLDRSLAEE NLTLFIHFFA KGHTALQITA LHILTDILVV HGAQLLSANP TLLKVYVKAL RSGAKHPELQ AVAVVAASKL LLGRVVSDRD VAAELLKTLV VAYFEPASAG NQSVRQALNY FLPVFCYSRA ENQDLMRRVA LDALHTLYNV REGFDDDDAD IDDEMVSLAT IGACLVDWTD PRKVYTPDAG GLQAEKHVNA DVHLDFAKAI LERLEGNVPR EEKKVLASLL SKLYISPASS PDKTRETYRV VSAAVDTNLL PDTTSRNALY KIHVSLGKIV NALDGAVPSV ETASVAGSTG TGIGTTTTAA TGR
Entity #1566Name: Ycg1 / Type: protein / Description: Condensin complex subunit 3-like protein / Formula weight: 108.3 / Num. of mol.: 2
Source: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
References: UniProt: G0SHK2
Sequence: PDNALRTQVT TVFREAQRTT ASHRKLVVTL RKIQEACCYE PATGQKQSGA AAALSEFDED DFNTEFVRCA LRILPVKKSE GVGEKTVRFV SLFLRHAIEK DNELLGDTQM DEDDADNSTM PETPSTRLTS HLMETILPML MAKDKFVRYR AAQLLAHIVN SLDAIDDDLY ...Sequence:
PDNALRTQVT TVFREAQRTT ASHRKLVVTL RKIQEACCYE PATGQKQSGA AAALSEFDED DFNTEFVRCA LRILPVKKSE GVGEKTVRFV SLFLRHAIEK DNELLGDTQM DEDDADNSTM PETPSTRLTS HLMETILPML MAKDKFVRYR AAQLLAHIVN SLDAIDDDLY QKLRAGLLRR IHDKEPMVRA QAVLGLGRLA GNALDEEPGA GDSDLDGDDD GPGGASGVSL MDKLMDVMKN DPSADVRRTL LVNLPITPKT LPSLLERARD QDPATRRAVY SRLLPALGDF RHLSLSMREK LLRWGLRDRD ENVRKAAARL FRERWIEDCA GTPRPEPGQP IELSPPNMDA LLELLERIDV VNSGGENGIA LEAMRGFWEG RPDYRDAMVF DDHFFETLSA ESVFALRTFN DFCRNEAGGK YEALIEEKLP EVTKLAFYLE RYIKVLIDAI KRAEEQGEEE EEDTAEQEFI VEQLLHIALT LDYADEVGRR KMFALLRQTL SIPELPEECT KLTVMVLRDL CAPDAAGEKE FTSVVLEAVA DVHDTIVDED DDDANVPAED DESFVSARSE VSRESTPAPG GGEGGGSNSR KRGRSPTPKM TEEEAAAKAI REIMINMKCL HIVQCMLQNV QGQLQSNDNL ISMLNNLVVP AVRSHEAPVR ERGLVCLGLC ALLDRSLAEE NLTLFIHFFA KGHTALQITA LHILTDILVV HGAQLLSANP TLLKVYVKAL RSGAKHPELQ AVAVVAASKL LLGRVVSDRD VAAELLKTLV VAYFEPASAG NQSVRQALNY FLPVFCYSRA ENQDLMRRVA LDALHTLYNV REGFDDDDAD IDDEMVSLAT IGACLVDWTD PRKVYTPDAG GLQAEKHVNA DVHLDFAKAI LERLEGNVPR EEKKVLASLL SKLYISPASS PDKTRETYRV VSAAVDTNLL PDTTSRNALY KIHVSLGKIV NALDGAVPSV ETASVAGSTG TGIGTTTTAA TGR

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / : Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 4 mm
DetectorName: Pilatus 2M
Scan
Title: Condensin complex subunit 3-like protein, Ycg1, at 0.5mg/ml
Measurement date: Oct 20, 2016 / Storage temperature: 13.1 °C / Cell temperature: 10.2 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0492 2.5149
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 784 /
MinMax
Q0.057567 1.68678
P(R) point1 784
R0 16.1
Result
Type of curve: single_conc
Comments: Ycg1 at 0.5mgl/ml was modeled as a volume-fraction weighted mixture consisting of 56% monomers and 44% dimers
ExperimentalStandardPorod
MW138 kDa146 kDa188 kDa
Volume--301 nm3

P(R)GuinierGuinier error
Forward scattering, I011400 11342 44
Radius of gyration, Rg4.853 nm4.74 nm0.17

MinMax
D-16.1
Guinier point5 109

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