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- SASDFJ3: Maltose binding protein-SpoIVB peptidase fusion (MBP-SpoIVB) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDFJ3
SampleMaltose binding protein-SpoIVB peptidase fusion (MBP-SpoIVB)
  • SpoIVB peptidase (MBP fusion) (protein), MBP-SpoIVB, Bacillus subtilis (strain 168)
Function / homology
Function and homology information


SpoIVB peptidase / sporulation resulting in formation of a cellular spore / serine-type peptidase activity / proteolysis
Similarity search - Function
Peptidase S55, SpoIVB / Peptidase S55, sporulation stage IV, protein B / SpoIVB peptidase S55 / SpoIVB peptidase domain profile. / PDZ domain 6 / PDZ domain / PDZ domain profile. / Domain present in PSD-95, Dlg, and ZO-1/2. / PDZ domain / PDZ superfamily / Peptidase S1, PA clan
Similarity search - Domain/homology
Biological speciesBacillus subtilis (strain 168) (bacteria)
Contact author
  • Longguang Jiang (Fuzhou University)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2832
Type: atomic / Chi-square value: 0.971 / P-value: 0.330455
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Maltose binding protein-SpoIVB peptidase fusion (MBP-SpoIVB)
Specimen concentration: 5 mg/ml
BufferName: 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol / pH: 8
Entity #1497Name: MBP-SpoIVB / Type: protein / Description: SpoIVB peptidase (MBP fusion) / Formula weight: 80.091 / Num. of mol.: 1 / Source: Bacillus subtilis (strain 168) / References: UniProt: P17896
Sequence: MHHHHHHKTE EGKLVIWING DKGYNGLAEV GKKFEKDTGI KVTVEHPDKL EEKFPQVAAT GDGPDIIFWA HDRFGGYAQS GLLAEITPAA AFQDKLYPFT WDAVRYNGKL IAYPIAVEAL SLIYNKDLLP NPPKTWEEIP ALDKELKAKG KSALMFNLQE PYFTWPLIAA ...Sequence:
MHHHHHHKTE EGKLVIWING DKGYNGLAEV GKKFEKDTGI KVTVEHPDKL EEKFPQVAAT GDGPDIIFWA HDRFGGYAQS GLLAEITPAA AFQDKLYPFT WDAVRYNGKL IAYPIAVEAL SLIYNKDLLP NPPKTWEEIP ALDKELKAKG KSALMFNLQE PYFTWPLIAA DGGYAFKYAA GKYDIKDVGV DNAGAKAGLT FLVDLIKNKH MNADTDYSIA EHAFNHGETA MTINGPWAWS NIDTSAVNYG VTVLPTFKGQ PSKPFVGVLS AGINAASPNK ELAKEFLENY LLTDEGLEAV NKDKPLGAVA LKSYEEELVK DPRVAATMEN AQKGEIMPNI PQMSAFWYAV RTAVINAASG RQTVDAALAA AQTNAAAASS ASGAPLEAAA TGKKSGESEL VYDLAGFPIK KTKVHVLPDL KVIPGGQSIG VKLHSVGVLV VGFHQINTSE GKKSPGETAG IEAGDIIIEM NGQKIEKMND VAPFIQKAGK TGESLDLLIK RDKQKIKTKL IPEKDEGEGK YRIGLYIRDS AAGIGTMTFY EPKTKKYGAL GHVISDMDTK KPIVVENGEI VKSTVTSIEK GTGGNPGEKL ARFSSERKTI GDINRNSPFG IFGTLHQPIQ NNISDQALPV AFSTEVKKGP AEILTVIDDD KVEKFDIEIV STTPQKFPAT KGMVLKITDP RLLKETGGIV QGMAGSPIIQ NGKVIGAVTH VFVNDPTSGY GVHIEWMLSE AGIDIYGKEK AS

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Experimental information

BeamInstrument name: Shanghai Synchrotron Radiation Facility (SSRF) BL19U2
City: Shanghai / 国: China / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 2.3 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Maltose binding protein-SpoIVB peptidase fusion (MBP-SpoIVB)
Measurement date: Jul 12, 2018 / Storage temperature: 4 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 20 / Unit: 1/A /
MinMax
Q0.007 0.3348
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 741 /
MinMax
Q0.0194648 0.307248
P(R) point1 741
R0 156
Result
Type of curve: single_conc
Comments: The originally deposited p(r) profile is included in the full entry zip archive.
ExperimentalPorod
MW77.6 kDa60 kDa
Volume-96 nm3

P(R)GuinierGuinier error
Forward scattering, I028.57 27.64 0.2
Radius of gyration, Rg4.04 nm3.72 nm0.05

MinMax
D-15.6
Guinier point33 72

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