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- SASDEW9: Cytohesin-2; ARF nucleotide-binding site opener, ARNO -

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Basic information

Entry
Database: SASBDB / ID: SASDEW9
SampleCytohesin-2; ARF nucleotide-binding site opener, ARNO
  • Cytohesin-2 ARF nucleotide-binding site opener (protein), ARNO, Homo sapiens
Function / homology
Function and homology information


Intra-Golgi traffic / inositol 1,4,5 trisphosphate binding / regulation of ARF protein signal transduction / bicellular tight junction / guanyl-nucleotide exchange factor activity / adherens junction / endocytosis / actin cytoskeleton organization / growth cone / Golgi membrane ...Intra-Golgi traffic / inositol 1,4,5 trisphosphate binding / regulation of ARF protein signal transduction / bicellular tight junction / guanyl-nucleotide exchange factor activity / adherens junction / endocytosis / actin cytoskeleton organization / growth cone / Golgi membrane / lipid binding / membrane / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Sec7 domain / Sec7, C-terminal domain superfamily / Sec7 domain superfamily / Sec7 domain / SEC7 domain profile. / Sec7 domain / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / PH-like domain superfamily
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
Contact author
  • Wenhua Zhang (CNRS UMR8113, ENS Paris-Saclay, Cachan, France)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #2192
Type: dummy / Radius of dummy atoms: 1.90 A / Chi-square value: 1.45
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Cytohesin-2; ARF nucleotide-binding site opener, ARNO / Specimen concentration: 10 mg/ml
BufferName: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl
pH: 7.5
Entity #1221Name: ARNO / Type: protein / Description: Cytohesin-2 ARF nucleotide-binding site opener / Formula weight: 46.6 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: Q99418
Sequence: MEDGVYEPPD LTPEERMELE NIRRRKQELL VEIQRLREEL SEAMSEVEGL EANEGSKTLQ RNRKMAMGRK KFNMDPKKGI QFLVENELLQ NTPEEIARFL YKGEGLNKTA IGDYLGEREE LNLAVLHAFV DLHEFTDLNL VQALRQFLWS FRLPGEAQKI DRMMEAFAQR ...Sequence:
MEDGVYEPPD LTPEERMELE NIRRRKQELL VEIQRLREEL SEAMSEVEGL EANEGSKTLQ RNRKMAMGRK KFNMDPKKGI QFLVENELLQ NTPEEIARFL YKGEGLNKTA IGDYLGEREE LNLAVLHAFV DLHEFTDLNL VQALRQFLWS FRLPGEAQKI DRMMEAFAQR YCLCNPGVFQ STDTCYVLSF AVIMLNTSLH NPNVRDKPGL ERFVAMNRGI NEGGDLPEEL LRNLYDSIRN EPFKIPEDDG NDLTHTFFNP DREGWLLKLG GGRVKTWKRR WFILTDNCLY YFEYTTDKEP RGIIPLENLS IREVDDPRKP NCFELYIPNN KGQLIKACKT EADGRVVEGN HMVYRISAPT QEEKDEWIKS IQAAVSVDPF YEMLAARKKR ISVKKKQEQP

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.993 Å
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Cytohesin-2; ARF nucleotide-binding site opener, ARNO
Measurement date: Jun 23, 2016 / Storage temperature: 15 °C / Cell temperature: 15 °C / Exposure time: 1 sec. / Number of frames: 1500 / Unit: 1/nm /
MinMax
Q0.0724 4.9518
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 500 /
MinMax
Q0.091234 2.44603
P(R) point1 500
R0 19.65
Result
Type of curve: single_conc /
ExperimentalPorod
MW94 kDa90 kDa
Volume-145 nm3

P(R)GuinierGuinier error
Forward scattering, I044.95 43.99 0.067
Radius of gyration, Rg5.107 nm4.75 nm0.1

MinMax
D-19.65
Guinier point5 43

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