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- SASDDZ8: Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral... -

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Basic information

Entry
Database: SASBDB / ID: SASDDZ8
SampleDesigned hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)
  • Fusion protein of LSm and MyoX-coil (protein), TIP60 protomer, Artificial protein
Biological speciesArtificial protein
CitationDate: 2018 Jul 31
Title: Design of hollow protein nanoparticles with modifiable interior and exterior surfaces
Authors: Kawakami N / Kondo H / Matsuzawa Y / Hayasaka K / Nasu E / Sasahara K / Arai R
Contact author
  • Ryoichi Arai (Shinshu University)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #2152
Type: dummy / Radius of dummy atoms: 8.25 A / Chi-square value: 0.012
Search similar-shape structures of this assembly by Omokage search (details)
Model #2153
Type: dummy / Radius of dummy atoms: 8.25 A / Chi-square value: 0.012
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)
Specimen concentration: 0.98-4.97
BufferName: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol / pH: 8
Entity #1158Name: TIP60 protomer / Type: protein / Description: Fusion protein of LSm and MyoX-coil / Formula weight: 17.766 / Source: Artificial protein
Sequence:
MGSSHHHHHH SQDPKNIKIM RLVTGEDIIG NISESQGLIT IKKAFVIIPM QATPGKPVQL VLSPWQPYTD DKEIVIDDSK VITITSPKDD IIKSYESHTR VLENKQVEEI LRLEKEIEDL QRMKEQQELS LTEASLQKLQ ERRDQELRRL EEE

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Experimental information

BeamInstrument name: Photon Factory (PF), High Energy Accelerator Research Organization (KEK) BL-6A
City: Tsukuba / 国: Japan / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.023 mm
DetectorName: Pilatus3 1M / Type: Reverse-biased silicon diode array / Pixsize x: 172 mm
Scan
Title: Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)
Measurement date: Nov 11, 2017 / Cell temperature: 25 °C / Exposure time: 5 sec. / Number of frames: 30 / Unit: 1/nm /
MinMax
Q0.057 2.603
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 221 /
MinMax
Q0.0676623 0.85099
P(R) point1 221
R0 23
Result
Type of curve: extrapolated
Comments: The ab initio dummy atom bead models displayed in this entry are: Top - a refined DAMMIN model and corresponding fit generated from 20 individual model spatial alignments; Bottom - the bead- ...Comments: The ab initio dummy atom bead models displayed in this entry are: Top - a refined DAMMIN model and corresponding fit generated from 20 individual model spatial alignments; Bottom - the bead-occupancy and volume-corrected (averaged) spatial representation of TIP60 (DAMFILT) obtained from 20 individual model spatial alignments.
ExperimentalStandard
MW1063 kDa1063 kDa

P(R)GuinierGuinier error
Forward scattering, I00.6241 0.643 0.001
Radius of gyration, Rg8.952 nm9.39 nm0.06

MinMax
D-23
Guinier point4 23

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