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- SASDDJ6: SAM-dependent O-Methyltransferase from Mycobacterium hassiacum -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDDJ6
SampleSAM-dependent O-Methyltransferase from Mycobacterium hassiacum
  • Methyltransferase domain protein (protein), Methyltransferase, Mycobacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)
Function / homologyMMP 1-O-methyltransferase / Methyltransferase domain / methyltransferase activity / methylation / carbohydrate metabolic process / S-adenosyl-L-methionine-dependent methyltransferase superfamily / metal ion binding / MMP 1-O-methyltransferase
Function and homology information
Biological speciesMycobacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849) (bacteria)
CitationDate: 2019 Jan 3
Title: Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides
Authors: Ripoll-Rozada J / Costa M / Manso J / Maranha A / Miranda V / Sequeira A / Ventura M / Macedo-Ribeiro S / Pereira P
Contact author
  • Jose A Manso (Instituto de Investigaçao e Inovaçao em Saúde (i3S-IBMC), Universidade do Porto)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2006
Type: atomic / Chi-square value: 3.664
Search similar-shape structures of this assembly by Omokage search (details)
Model #2009
Type: dummy / Software: (r8972) / Radius of dummy atoms: 1.80 A / Chi-square value: 8.443
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: SAM-dependent O-Methyltransferase from Mycobacterium hassiacum
Specimen concentration: 1.61-3.22
BufferName: 20 mM bis-tris propane, 50 mM NaCl, 2 mM DTT, 5% (v/v) glycerol
pH: 7.5
Entity #1070Name: Methyltransferase / Type: protein / Description: Methyltransferase domain protein / Formula weight: 25.409 / Num. of mol.: 2
Source: Mycobacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)
References: UniProt: K5B7F3
Sequence: MTDIRDTDAL FALADRVTGF MPADEGRTLY ETAVRYLGDG VGVEIGTYCG KSTVLLGAAA RQTGGVVFTV DHHHGSEEHQ PGWEYHDPSL VDPVTGLFDT LPRLRHTLDE ADLYDHVVAV VGKSAVVARG WRTPLRFLFI DGGHTEEAAQ RDFDGWARWV EVGGALVIHD ...Sequence:
MTDIRDTDAL FALADRVTGF MPADEGRTLY ETAVRYLGDG VGVEIGTYCG KSTVLLGAAA RQTGGVVFTV DHHHGSEEHQ PGWEYHDPSL VDPVTGLFDT LPRLRHTLDE ADLYDHVVAV VGKSAVVARG WRTPLRFLFI DGGHTEEAAQ RDFDGWARWV EVGGALVIHD VFPDPKDGGQ APFHIYQRAL NTGDFREVNA YGSMRVLERT SGIAGQPLKL AAALEHHHHH H

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.867 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: SAM-dependent O-Methyltransferase from Mycobacterium hassiacum
Measurement date: Jun 21, 2017 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/A /
MinMax
Q0.0078 0.4939
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 611 /
MinMax
Q0.0120813 0.301084
P(R) point1 611
R0 85
Result
Type of curve: extrapolated
ExperimentalStandardPorod
MW44.855 kDa44.855 kDa54 kDa
Volume--86.4 nm3

P(R)GuinierGuinier error
Forward scattering, I042.46 42.58 0.05
Radius of gyration, Rg2.56 nm2.57 nm0.02

MinMax
D-8.5
Guinier point10 85

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