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- SASDDG3: Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) in Com... -

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Basic information

Entry
Database: SASBDB / ID: SASDDG3
SampleSolution Structure of Archaeal Biofilm Regulator 2 (AbfR2) in Complex with DNA
  • Sa0446 binding sequence 40bp (DNA), 40bp dsDNA
  • Transcriptional regulator Lrs14-like protein (protein), AbfR2, Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Function / homologyTranscription regulator TrmB, N-terminal / Sugar-specific transcriptional regulator TrmB / helix_turn_helix, Arsenical Resistance Operon Repressor / HTH ArsR-type DNA-binding domain / ArsR-like helix-turn-helix domain / Winged helix DNA-binding domain superfamily / DNA-binding transcription factor activity / Winged helix-like DNA-binding domain superfamily / Transcriptional regulator Lrs14-like protein
Function and homology information
Biological speciesSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (acidophilic)
Contact author
  • Marian Vogt (University of Marburg, Marburg, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1781
Type: dummy / Radius of dummy atoms: 2.70 A / Chi-square value: 0.861
Search similar-shape structures of this assembly by Omokage search (details)
Model #1782
Type: dummy / Radius of dummy atoms: 2.70 A / Chi-square value: 0.830 / P-value: 0.000013
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) in Complex with DNA
Specimen concentration: 0.63 mg/ml / Entity id: 869 / 919
BufferName: 300 mM NaCl, 20 mM HEPES, pH 7.5 / pH: 7.5
Entity #869Name: 40bp dsDNA / Type: DNA / Description: Sa0446 binding sequence 40bp / Formula weight: 24.721 / Num. of mol.: 1
Sequence:
GTAGTAAGAT TTAAAAAAAC ATACTAAGAT ATAAATTAAC GTTAATTTAT ATCTTAGTAT GTTTTTTTAA ATCTTACTAC
Entity #919Name: AbfR2 / Type: protein / Description: Transcriptional regulator Lrs14-like protein / Formula weight: 16.368 / Num. of mol.: 2
Source: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
References: UniProt: Q4J9G1
Sequence:
MGSSHHHHHH SQDPNSMETV LQIPYQKKTQ IEKLLEFMYG LNEKEVQLIF RLLYSDTKLN IEELAEEFKV SKALISKSLS ELANKGLIER EKVSNEGRKG RPIYVYYVDR EQLFKRISRD LEELVQASIA KLKEYIFKS

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.124 Å
DetectorName: Pilatus 1M
Scan
Title: Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) in Complex with DNA
Measurement date: Nov 5, 2016 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0348 4.9439
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 470 /
MinMax
Q0.114886 2.32447
P(R) point1 470
R0 12.8
Result
Type of curve: merged
ExperimentalStandardPorod
MW41.981 kDa67.74 kDa-
Volume--60.38 nm3

P(R)GuinierGuinier error
Forward scattering, I068.09 67.74 0.17
Radius of gyration, Rg3.561 nm3.44 nm0.81

MinMax
D-12.8
Guinier point18 64

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