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- SASDD43: Mycobacterial suicide toxin (MbsT) (Mycobacterial suicide toxin, MbsT) -

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Basic information

Entry
Database: SASBDB / ID: SASDD43
SampleMycobacterial suicide toxin (MbsT)
  • Mycobacterial suicide toxin (protein), MbsT, Mycobacterium tuberculosis H37Rv
Function / homologyRES domain / RES domain / RES / Transferases; Glycosyltransferases; Pentosyltransferases / nucleotidyltransferase activity / NAD(+) phosphorylase MbcT
Function and homology information
Biological speciesMycobacterium tuberculosis H37Rv (bacteria)
Contact author
  • Diana Freire (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1754
Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 1.221 / P-value: 0.569452
Search similar-shape structures of this assembly by Omokage search (details)
Model #1755
Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 2.204
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Mycobacterial suicide toxin (MbsT) / Specimen concentration: 0.40-7.40
BufferName: 30 mM Tris-HCl, 200 mM NaCl, 10% glycerol / pH: 7.5
Entity #950Name: MbsT / Type: protein / Description: Mycobacterial suicide toxin / Formula weight: 20.396 / Num. of mol.: 1 / Source: Mycobacterium tuberculosis H37Rv / References: UniProt: P9WLP9
Sequence:
MDSDALDEGL VQRIDARGTI EWSETCYRYT GAHRDALSGE GARRFGGRWN PPLLFPAIYL ADSAQACMVE VERAAQAAST TAEKMLEAAY RLHTIDVTDL AVLDLTTPQA REAVGLENDD IYGDDWSGCQ AVGHAAWFLH MQGVLVPAAG GVGLVVTAYE QRTRPGQLQL RQSVDLTPAL YQELRAT

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 2 mm
DetectorName: Pilatus 2M
Scan
Title: Mycobacterial suicide toxin (MbsT) / Measurement date: May 2, 2017 / Storage temperature: 20 °C / Cell temperature: 20.3 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.1079 4.8347
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1720 /
MinMax
Q0.107882 4.83472
P(R) point1 1720
R0 5.6
Result
Type of curve: single_conc
ExperimentalStandardStandard errorPorod
MW18 kDa18 kDa2 17 kDa
Volume---30 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I018610 14 18612.1 21
Radius of gyration, Rg1.747 nm0.002 1.75 nm0.1

MinMax
D-5.6
Guinier point1 231

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