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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDD32 |
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![]() | DNA-(adenine N6)-methyltransferase from Acinetobacter baumannii ATCC 17978
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生物種 | ![]() |
![]() | ![]() タイトル: Recombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity. 著者: Ulrike Blaschke / Beneditta Suwono / Sachli Zafari / Ingo Ebersberger / Evelyn Skiebe / Cy M Jeffries / Dmitri I Svergun / Gottfried Wilharm / ![]() 要旨: Acinetobacter baumannii appears as an often multidrug-resistant nosocomial pathogen in hospitals worldwide. Its remarkable persistence in the hospital environment is probably due to intrinsic and ...Acinetobacter baumannii appears as an often multidrug-resistant nosocomial pathogen in hospitals worldwide. Its remarkable persistence in the hospital environment is probably due to intrinsic and acquired resistance to disinfectants and antibiotics, tolerance to desiccation stress, capability to form biofilms, and is possibly facilitated by surface-associated motility. Our attempts to elucidate surface-associated motility in A. baumannii revealed a mutant inactivated in a putative DNA-(adenine N6)-methyltransferase, designated A1S_0222 in strain ATCC 17978. We recombinantly produced A1S_0222 as a glutathione S-transferase (GST) fusion protein and purified it to near homogeneity through a combination of GST affinity chromatography, cation exchange chromatography and PD-10 desalting column. Furthermore we demonstrate A1S_0222-dependent adenine methylation at a GAATTC site. We propose the name AamA (Acinetobacteradenine methyltransferase A) in addition to the formal names M.AbaBGORF222P/M.Aba17978ORF8565P. Small angle X-ray scattering (SAXS) revealed that the protein is monomeric and has an extended and likely two-domain shape in solution. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #1703 | ![]() タイプ: dummy / ダミー原子の半径: 2.75 A / 対称性: P1 / カイ2乗値: 1.062 / P-value: 0.132008 ![]() |
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モデル #1704 | ![]() タイプ: dummy / ダミー原子の半径: 2.75 A / 対称性: P1 / カイ2乗値: 1.062 / P-value: 0.132008 ![]() |
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試料
![]() | 名称: DNA-(adenine N6)-methyltransferase from Acinetobacter baumannii ATCC 17978 試料濃度: 2.4 mg/ml |
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バッファ | 名称: 150mM NaCl, 10mM Tris, 1mM DTT, 5% v/v glycerol / pH: 7.4 |
要素 #912 | 名称: A1S_0222 / タイプ: protein / 記述: DNA-(adenine N6)-methyltransferase / 分子量: 48.902 / 分子数: 1 / 由来: Acinetobacter baumannii ATCC 17978 配列: MNSEPSVYHK RRHAARTTDE YLFHQLVPYL GNKRRLLHLI LEALEITGTL NSKKKNPPIF ADFFAGSGVV SRLARQNGYR VIANDWEPYS HALNHAILAC VDAPAFKELG GYQKAIDYLN RLPEVKGWVT HNLCPRNDDV YDPSRDRLFF KRRNGMRIDA IRQQIATWQA ...配列: MNSEPSVYHK RRHAARTTDE YLFHQLVPYL GNKRRLLHLI LEALEITGTL NSKKKNPPIF ADFFAGSGVV SRLARQNGYR VIANDWEPYS HALNHAILAC VDAPAFKELG GYQKAIDYLN RLPEVKGWVT HNLCPRNDDV YDPSRDRLFF KRRNGMRIDA IRQQIATWQA QGAINDVEMS ALLAPLLYSA SFVSNTSGVF KSFHQGWGGR TQTALERIES LLWLTPSRFC EIGDRKRPAA EMWCVDAQHL ANQMSGFEVD VAYLDPPYNQ HAYSSNYHVL NALTLWDQVD LPPPDTKGYK SGIDRAWRKE RPSPYNSSKH AKDAYEKLLS TINARYILTS YSTDGNIEPK DLLMANLERG KVTLLTQDVP RYRVSKQRQS ERARVLEFIV ITDTHAKPGP PLRQLLGQLY HFAELGGVDT SGNSTQLALW |
-実験情報
ビーム | 設備名称: PETRA III EMBL P12 / 地域: Hamburg / 国: Germany ![]() | ||||||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | ||||||||||||||||||||||||||||||||||||
スキャン | 測定日: 2016年9月30日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 0.05 sec. / フレーム数: 20 / 単位: 1/nm /
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距離分布関数 P(R) |
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結果 | コメント: The individual DAMMIN models used to generate that averaged spatial representation of the protein (DAMFILT model) are included in the full entry zip archive as well as the refinement of ...コメント: The individual DAMMIN models used to generate that averaged spatial representation of the protein (DAMFILT model) are included in the full entry zip archive as well as the refinement of the averaged structure using DAMSTART.
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