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- SASDC32: Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB) -

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Basic information

Entry
Database: SASBDB / ID: SASDC32
SampleEthylene Receptor 1 Cytosolic Domain (in 250 mM NDSB)
  • Ethylene Receptor 1Ethylene as a plant hormone (protein), ETR1(-ΔTM), Arabidopsis thaliana
Function / homology
Function and homology information


ethylene receptor activity / regulation of seedling development / detection of ethylene stimulus / ethylene binding / seed dormancy process / defense response by callose deposition in cell wall / negative regulation of ethylene-activated signaling pathway / sugar mediated signaling pathway / response to gibberellin / response to insect ...ethylene receptor activity / regulation of seedling development / detection of ethylene stimulus / ethylene binding / seed dormancy process / defense response by callose deposition in cell wall / negative regulation of ethylene-activated signaling pathway / sugar mediated signaling pathway / response to gibberellin / response to insect / phloem or xylem histogenesis / response to ethylene / cytokinin metabolic process / regulation of stomatal movement / response to auxin / protein histidine kinase activity / response to abscisic acid / hydrogen peroxide biosynthetic process / histidine kinase / phosphorelay sensor kinase activity / response to salt stress / defense response / response to molecule of bacterial origin / response to heat / defense response to bacterium / cell division / endoplasmic reticulum membrane / endoplasmic reticulum / ATP binding / identical protein binding / metal ion binding
Similarity search - Function
Ethylene receptor / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain / Histidine kinase domain profile. / Response regulator receiver domain / cheY-homologous receiver domain ...Ethylene receptor / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain / Histidine kinase domain profile. / Response regulator receiver domain / cheY-homologous receiver domain / Signal transduction response regulator, receiver domain / Response regulatory domain profile. / GAF domain / Domain present in phytochromes and cGMP-specific phosphodiesterases. / CheY-like superfamily / GAF domain / GAF-like domain superfamily / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / Histidine kinase-like ATPases / Histidine kinase/HSP90-like ATPase / Histidine kinase/HSP90-like ATPase superfamily
Similarity search - Domain/homology
Biological speciesArabidopsis thaliana (thale cress)
CitationDate: 2015 Jan 30
Title: Structural Model of the Cytosolic Domain of the Plant Ethylene Receptor 1 (ETR1)
Authors: Mayerhofer H / Panneerselvam S / Kaljunen H / Tuukkanen A / Mertens H
Contact author
  • Anne Tuukkanen
  • Haydyn Mertens

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1495
Type: atomic / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Chi-square value: 2.1316
Search similar-shape structures of this assembly by Omokage search (details)
Model #1496
Type: dummy / Software: (1.44) / Radius of dummy atoms: 4.50 A
Comment: Global refinement of GAF-DHp-CA-RD + DHp-CA-RD + DHp-CA data (3 sets)
Chi-square value: 1.234321
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB) / Dry vol: 78367 / Specimen concentration: 0.70-2.70 / Concentration method: A280
BufferName: 20 mM Tris-NDSB 150 mM NaCl 1mM DTT 250 mM NDSB / Concentration: 20.00 mM / pH: 8.8 / Composition: 150 mM NaCl, 1mM DTT, 250 mM NDSB
Entity #515Name: ETR1(-ΔTM) / Type: protein / Description: Ethylene Receptor 1Ethylene as a plant hormone / Formula weight: 64.555 / Num. of mol.: 2 / Source: Arabidopsis thaliana / References: UniProt: P49333
Sequence: DRHTILKTTL VELGRTLALE ECALWMPTRT GLELQLSYTL RHQHPVEYTV PIQLPVINQV FGTSRAVKIS PNSPVARLRP VSGKYMLGEV VAVRVPLLHL SNFQINDWPE LSTKRYALMV LMLPSDSARQ WHVHELELVE VVADQVAVAL SHAAILEESM RARDLLMEQN ...Sequence:
DRHTILKTTL VELGRTLALE ECALWMPTRT GLELQLSYTL RHQHPVEYTV PIQLPVINQV FGTSRAVKIS PNSPVARLRP VSGKYMLGEV VAVRVPLLHL SNFQINDWPE LSTKRYALMV LMLPSDSARQ WHVHELELVE VVADQVAVAL SHAAILEESM RARDLLMEQN VALDLARREA ETAIRARNDF LAVMNHEMRT PMHAIIALSS LLQETELTPE QRLMVETILK SSNLLATLMN DVLDLSRLED GSLQLELGTF NLHTLFREVL NLIKPIAVVK KLPITLNLAP DLPEFVVGDE KRLMQIILNI VGNAVKFSKQ GSISVTALVT KSDTRAADFF VVPTGSHFYL RVKVKDSGAG INPQDIPKIF TKFAQTQSLA TRSSGGSGLG LAISKRFVNL MEGNIWIESD GLGKGCTAIF DVKLGISERS NESKQSGIPK VPAIPRHSNF TGLKVLVMDE NGVSRMVTKG LLVHLGCEVT TVSSNEECLR VVSHEHKVVF MDVCMPGVEN YQIALRIHEK FTKQRHQRPL LVALSGNTDK STKEKCMSFG LDGVLLKPVS LDNIRDVLSD LLEPRVLYEG M

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Experimental information

BeamInstrument name: DORIS III X33 / City: Hamburg / : Germany / Shape: 0.6 / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 1M-W / Pixsize x: 0.172 mm
Scan
Title: ETR1-delTM_NDSB / Measurement date: Mar 19, 2011 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 15 sec. / Number of frames: 8 / Unit: 1/nm /
MinMax
Q0.1409 6.0241
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 400 /
MinMax
Q0.1423 2.328
P(R) point1 400
R0 15.8
Result
Type of curve: extrapolated / Standard: BSA / Comments: Cytosolic domain of ETR1 in 250 mM NBSB
ExperimentalExperimental errorStandardStandard errorPorodPorod error
MW54.09 kDa5 54.09 kDa5 186 kDa20
Volume----316 nm350

P(R)P(R) errorGuinierGuinier error
Forward scattering, I072.3 0.3 67.8 0.7
Radius of gyration, Rg5.2 nm0.1 4.7 nm0.2

MinMaxError
D-15.8 0.5
Guinier point1 47 -

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