SASDAQ5: Lumazine Synthase

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAQ5

試料

Lumazine Synthase

• Lumazine Synthase (protein), Lumazine Synthase, B. subtilis

• 生物種: B. subtilis
• 引用: ジャーナル: J Mol Biol / 年: 2006; タイトル: Multiple assembly states of lumazine synthase: a model relating catalytic function and molecular assembly.; 著者: Xiaofeng Zhang / Petr V Konarev / Maxim V Petoukhov / Dmitri I Svergun / Li Xing / R Holland Cheng / Ilka Haase / Markus Fischer / Adelbert Bacher / Rudolf Ladenstein / Winfried Meining / ; 要旨: Lumazine synthases have been observed in the form of pentamers, dimers of pentamers, icosahedral capsids consisting of 60 subunits and larger capsids with unknown molecular structure. Here we describe the analysis of the assembly of native and mutant forms of lumazine synthases from Bacillus subtilis and Aquifex aeolicus at various pH values and in the presence of different buffers using small angle X-ray scattering and electron microscopy. Both wild-type lumazine synthases are able to form capsids with a diameter of roughly 160 A and larger capsids with diameters of around 300 A. The relative abundance of smaller and larger capsids is strongly dependent on buffer and pH. Both forms can co-exist and are in some cases accompanied by other incomplete or deformed capsids. Several mutants of the B. subtilis lumazine synthase, in which residues in or close to the active site were replaced, as well as an insertion mutant of A. aeolicus lumazine synthase form partially or exclusively larger capsids with a diameter of about 300 A. The mutations also reduce or inhibit enzymatic activity, suggesting that the catalytic function of the enzyme is tightly correlated with its quaternary structure. The data show that multiple assembly forms are a general feature of lumazine synthases.

登録者

• Petr Konarev (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #135: ; タイプ: dummy / ソフトウェア: dammin / ダミー原子の半径: 1.90 A / 対称性: Icosohedra / カイ2乗値: 3942.960849; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Lumazine Synthase / Sample MW: 960 kDa
• バッファ: 名称: Borate buffer / pH: 7

要素 #98

名称: Lumazine Synthase / タイプ: protein / 記述: Lumazine Synthase / 分子量: 16.29 / 由来: B. subtilis
配列:

MNIIQGNLVG TGLKIGIVVG RFNDFITSKL LSGAEDALLR HGVDTNDIDV AWVPGAFEIP FAAKKMAETK KYDAIITLGT VIRGATTHYD YVCNEAAKGI AQAANTTGVP VIFGIVTTEN IEQAIERAGT KAGNKGVDCA VSAIEMANLN RSFE

実験情報

• ビーム: 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron
• 検出器: 名称: MAR 345 Image Plate

スキャン

タイトル: Lumazine Syntahse in Borate Buffer / 測定日: 2004年11月25日 / 保管温度: 15 °C / セル温度: 15 °C / 照射時間: 120 sec. / フレーム数: 2 / 単位: 1/nm /

	Min	Max
Q	0.1056	3.328

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.5a / ポイント数: 392 /

	Min	Max
Q	0.1858	3.326
P(R) point	30	421
R	0	15.5

結果

D max: 15.5 / カーブのタイプ: single_conc /

	実験値	Porod
分子量	960 kDa	-
体積	-	1450 nm3

	P(R)	Guinier
前方散乱 I0	581400	607904
慣性半径, Rg	6.18 nm	6.65 nm