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- SASDCJ3: Bovine Serum Albumin (Serum albumin) -

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Basic information

Entry
Database: SASBDB / ID: SASDCJ3
SampleBovine Serum Albumin
  • Serum albumin (protein), Bos taurus
Function / homology
Function and homology information


enterobactin binding / cellular response to calcium ion starvation / negative regulation of mitochondrial depolarization / toxic substance binding / cellular response to starvation / fatty acid binding / pyridoxal phosphate binding / protein-containing complex / DNA binding / extracellular space ...enterobactin binding / cellular response to calcium ion starvation / negative regulation of mitochondrial depolarization / toxic substance binding / cellular response to starvation / fatty acid binding / pyridoxal phosphate binding / protein-containing complex / DNA binding / extracellular space / extracellular region / metal ion binding / cytoplasm
Similarity search - Function
Serum albumin/Alpha-fetoprotein/Afamin / ALB/AFP/VDB / Serum albumin, N-terminal / Serum albumin, conserved site / Serum albumin-like / Serum albumin family / Albumin domain signature. / Albumin domain profile. / serum albumin
Similarity search - Domain/homology
Biological speciesBos taurus (cattle)
CitationJournal: Acta Crystallogr D Struct Biol / Year: 2017
Title: 2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update.
Authors: Jill Trewhella / Anthony P Duff / Dominique Durand / Frank Gabel / J Mitchell Guss / Wayne A Hendrickson / Greg L Hura / David A Jacques / Nigel M Kirby / Ann H Kwan / Javier Pérez / Lois ...Authors: Jill Trewhella / Anthony P Duff / Dominique Durand / Frank Gabel / J Mitchell Guss / Wayne A Hendrickson / Greg L Hura / David A Jacques / Nigel M Kirby / Ann H Kwan / Javier Pérez / Lois Pollack / Timothy M Ryan / Andrej Sali / Dina Schneidman-Duhovny / Torsten Schwede / Dmitri I Svergun / Masaaki Sugiyama / John A Tainer / Patrice Vachette / John Westbrook / Andrew E Whitten /
Abstract: In 2012, preliminary guidelines were published addressing sample quality, data acquisition and reduction, presentation of scattering data and validation, and modelling for biomolecular small-angle ...In 2012, preliminary guidelines were published addressing sample quality, data acquisition and reduction, presentation of scattering data and validation, and modelling for biomolecular small-angle scattering (SAS) experiments. Biomolecular SAS has since continued to grow and authors have increasingly adopted the preliminary guidelines. In parallel, integrative/hybrid determination of biomolecular structures is a rapidly growing field that is expanding the scope of structural biology. For SAS to contribute maximally to this field, it is essential to ensure open access to the information required for evaluation of the quality of SAS samples and data, as well as the validity of SAS-based structural models. To this end, the preliminary guidelines for data presentation in a publication are reviewed and updated, and the deposition of data and associated models in a public archive is recommended. These guidelines and recommendations have been prepared in consultation with the members of the International Union of Crystallography (IUCr) Small-Angle Scattering and Journals Commissions, the Worldwide Protein Data Bank (wwPDB) Small-Angle Scattering Validation Task Force and additional experts in the field.
Contact author
  • Jill Trewhella (School of Molecular Bioscience, University of Sydney., Sydney, New South Wales 2006, Australia)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1249
Type: dummy / Software: (on-line) / Radius of dummy atoms: 2.20 A / Symmetry: P1 / Chi-square value: 0.853 / P-value: 0.156000
Search similar-shape structures of this assembly by Omokage search (details)
Model #1251
Type: atomic / Software: (on-line) / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Multi-FoXS 1-state model based on PDB:4F5S chain A with residues 187-183 & 381-384 flexible
Chi-square value: 1.05151837959 / P-value: 0.023000
Search similar-shape structures of this assembly by Omokage search (details)
Model #1254
Type: atomic / Software: (on-line) / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Multi-FoXS e1-0 3-state model based on PDB:4F5S chain A with residues 187-183 & 381-384 flexible
Chi-square value: 0.836161911667 / P-value: 0.170900
Search similar-shape structures of this assembly by Omokage search (details)
Model #1255
Type: atomic / Software: (on-line) / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Multi-FoXS e1-1 3-state model based on PDB:4F5S chain A with residues 187-183 & 381-384 flexible
Chi-square value: 0.836161911667 / P-value: 0.170900
Search similar-shape structures of this assembly by Omokage search (details)
Model #1256
Type: atomic / Software: (on-line) / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Multi-FoXS e1-2 3-state model based on PDB:4F5S chain A with residues 187-183 & 381-384 flexible
Chi-square value: 0.836161911667 / P-value: 0.170900
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Bovine Serum Albumin / Specimen concentration: 0.56 mg/ml
BufferName: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3
pH: 7.5
Entity #671Type: protein / Description: Serum albumin / Formula weight: 66.432 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: P02769
Sequence: DTHKSEIAHR FKDLGEEHFK GLVLIAFSQY LQQCPFDEHV KLVNELTEFA KTCVADESHA GCEKSLHTLF GDELCKVASL RETYGDMADC CEKQEPERNE CFLSHKDDSP DLPKLKPDPN TLCDEFKADE KKFWGKYLYE IARRHPYFYA PELLYYANKY NGVFQECCQA ...Sequence:
DTHKSEIAHR FKDLGEEHFK GLVLIAFSQY LQQCPFDEHV KLVNELTEFA KTCVADESHA GCEKSLHTLF GDELCKVASL RETYGDMADC CEKQEPERNE CFLSHKDDSP DLPKLKPDPN TLCDEFKADE KKFWGKYLYE IARRHPYFYA PELLYYANKY NGVFQECCQA EDKGACLLPK IETMREKVLA SSARQRLRCA SIQKFGERAL KAWSVARLSQ KFPKAEFVEV TKLVTDLTKV HKECCHGDLL ECADDRADLA KYICDNQDTI SSKLKECCDK PLLEKSHCIA EVEKDAIPEN LPPLTADFAE DKDVCKNYQE AKDAFLGSFL YEYSRRHPEY AVSVLLRLAK EYEATLEECC AKDDPHACYS TVFDKLKHLV DEPQNLIKQN CDQFEKLGEY GFQNALIVRY TRKVPQVSTP TLVEVSRSLG KVGTRCCTKP ESERMPCTED YLSLILNRLC VLHEKTPVSE KVTKCCTESL VNRRPCFSAL TPDETYVPKA FDEKLFTFHA DICTLPDTEK QIKKQTALVE LLKHKPKATE EQLKTVMENF VAFVDKCCAA DDKEACFAVE GPKLVVSTQT ALA

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Experimental information

BeamInstrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / : Australia / Shape: Point / Type of source: X-ray synchrotron / Wavelength: 0.10332 Å / Dist. spec. to detc.: 2.683 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Bovine Serum Albumin / Measurement date: Mar 9, 2017 / Cell temperature: 22 °C / Exposure time: 1 sec. / Number of frames: 24 / Unit: 1/A /
MinMax
Q0.0066 0.3104
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 354 /
MinMax
Q0.00662753 0.281638
P(R) point1 354
R0 87.03
Result
Type of curve: single_conc
ExperimentalStandardPorod
MW66.43 kDa65.12 kDa67.333 kDa
Volume--101 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.04249 3.4E-5 0.04245 4.2E-5
Radius of gyration, Rg2.832 nm0.003 2.833 nm0.005

MinMax
D-8.7
Guinier point1 51

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