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- SASDCA3: Zinc finger and BTB domain-containing protein 38 (ZBTB38) complex... -
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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDCA3 |
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![]() | Zinc finger and BTB domain-containing protein 38 (ZBTB38) complexed with methylated C-terminal ZBTB38 binding sequence
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Function / homology | ![]() methyl-CpG binding / regulation of DNA replication / chromosome / blood microparticle / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA damage response / regulation of DNA-templated transcription ...methyl-CpG binding / regulation of DNA replication / chromosome / blood microparticle / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA damage response / regulation of DNA-templated transcription / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / metal ion binding Similarity search - Function |
Biological species | ![]() |
![]() | ![]() Title: The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. Authors: Amir Pozner / Nicholas O Hudson / Jill Trewhella / Tommy W Terooatea / Sven A Miller / Bethany A Buck-Koehntop / ![]() ![]() Abstract: Methyl-CpG binding proteins play an essential role in translating DNA methylation marks into a downstream transcriptional response, which has implications for both normal cell function as well as ...Methyl-CpG binding proteins play an essential role in translating DNA methylation marks into a downstream transcriptional response, which has implications for both normal cell function as well as disease. Although for many of these proteins, a detailed mechanistic understanding for how this cellular process is mediated remains to be determined. ZBTB38 is an under-characterized member of the zinc finger (ZF) family of methyl-CpG binding proteins. Functional knowledge has been gained for its conserved methylated DNA binding N-terminal ZF region; however, a specific role for the C-terminal set of five ZFs remains to be elucidated. Here we demonstrate for the first time that a subset of the C-terminal ZBTB38 ZFs exhibit high-affinity DNA interactions and that preferential targeting of the consensus DNA site is methyl specific. Utilizing a hybrid approach, a model for the C-terminal ZBTB38 ZFs in complex with its cognate DNA target is proposed, providing insight into a possible novel mode of methylated DNA recognition. Furthermore, it is shown that the C-terminal ZFs of ZBTB38 can directly occupy promoters harboring the newly identified sequence motif in cell in a methyl-dependent manner and, depending on the gene context, contribute to modulating transcriptional response. Combined, these findings provide evidence for a key and novel physiological function for the C-terminal ZF domain of ZBTB38. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data |
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External links
Related items in Molecule of the Month |
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-Models
Model #1238 | ![]() Type: dummy / Software: (1.45) / Radius of dummy atoms: 2.75 A / Comment: ZBTB38(1006-1153)-mCZ38BS complex / Chi-square value: 1.268 / P-value: 0.123700 ![]() |
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Model #1237 | ![]() Type: atomic / Software: (2.2) / Symmetry: P1 / Comment: ZBTB38(1006-1153)-mCZ38BS complex / Chi-square value: 1.739 / P-value: 0.027726 ![]() |
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Sample
![]() | Name: Zinc finger and BTB domain-containing protein 38 (ZBTB38) complexed with methylated C-terminal ZBTB38 binding sequence Specimen concentration: 3.4 mg/ml / Entity id: 663 / 667 |
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Buffer | Name: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O pH: 6.8 Comment: Solvent blank taken from concentration step flow through |
Entity #663 | Name: ZBTB38 / Type: protein Description: Zinc finger and BTB domain-containing protein 38 Formula weight: 17.337 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q8NAP3 Sequence: TSRPYACELC AKQFQSPSTL KMHMRCHTGE KPYQCKTCGR CFSVQGNLQK HERIHLGLKE FVCQYCNKAF TLNETLKIHE RIHTGEKRYH CQFCFQRFLY LSTKRNHEQR HIREHNGKGY ACFQCPKICK TAAALGMHQK KHLFKSP |
Entity #667 | Name: mZBTB38BS / Type: DNA / Description: methylated C-terminal ZBTB38 binding sequence / Formula weight: 16.705 / Num. of mol.: 1 Sequence: GCACTCATCG GCGCAGATCA GCTAGCCGGC TAGCTGATCT GCGCCGATGA GTGC |
-Experimental information
Beam | Instrument name: Department of Chemistry, University of Utah Anton Paar SAXSess City: Salt Lake City, UT / 国: USA ![]() | ||||||||||||||||||||||||||||||||||||||||||
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Detector | Name: Dectris Mythen 1K / Type: 1D diode array detector / Pixsize x: 50 mm | ||||||||||||||||||||||||||||||||||||||||||
Scan | Measurement date: Nov 11, 2016 / Cell temperature: 22 °C / Exposure time: 30 sec. / Number of frames: 120 / Unit: 1/nm /
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Distance distribution function P(R) |
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Result |
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