SASDC52: Bruton tyrosine kinase (Tyrosine-protein kinase BTK (R28C mutant)...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDC52

試料

Bruton tyrosine kinase

• Tyrosine-protein kinase BTK (R28C mutant) (protein), BTK, Homo sapiens

機能・相同性

分子機能:

regulation of B cell cytokine production / proteoglycan catabolic process / monocyte proliferation / positive regulation of interleukin-17A production / eosinophil homeostasis / regulation of B cell apoptotic process / positive regulation of type III hypersensitivity / B cell affinity maturation / positive regulation of synoviocyte proliferation / histamine secretion by mast cell / neutrophil homeostasis / positive regulation of cGAS/STING signaling pathway / cellular response to molecule of fungal origin / positive regulation of type I hypersensitivity / cellular response to interleukin-7 / MyD88 deficiency (TLR2/4) / IRAK4 deficiency (TLR2/4) / MyD88-dependent toll-like receptor signaling pathway / MyD88:MAL(TIRAP) cascade initiated on plasma membrane / positive regulation of B cell differentiation / positive regulation of immunoglobulin production / phospholipase activator activity / negative regulation of interleukin-10 production / negative regulation of B cell proliferation / positive regulation of NLRP3 inflammasome complex assembly / Fc-epsilon receptor signaling pathway / mesoderm development / phospholipase binding / phosphatidylinositol-3,4,5-trisphosphate binding / B cell activation / RHO GTPases Activate WASPs and WAVEs / cell maturation / positive regulation of phagocytosis / positive regulation of B cell proliferation / FCERI mediated Ca+2 mobilization / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / cellular response to reactive oxygen species / non-membrane spanning protein tyrosine kinase activity / B cell receptor signaling pathway / FCGR3A-mediated phagocytosis / non-specific protein-tyrosine kinase / apoptotic signaling pathway / calcium-mediated signaling / peptidyl-tyrosine phosphorylation / Regulation of actin dynamics for phagocytic cup formation / positive regulation of interleukin-6 production / G beta:gamma signalling through BTK / positive regulation of NF-kappaB transcription factor activity / positive regulation of tumor necrosis factor production / DAP12 signaling / G alpha (12/13) signalling events / T cell receptor signaling pathway / ER-Phagosome pathway / protein tyrosine kinase activity / cytoplasmic vesicle / G alpha (q) signalling events / adaptive immune response / response to lipopolysaccharide / Potential therapeutics for SARS / intracellular signal transduction / protein phosphorylation / membrane raft / innate immune response / perinuclear region of cytoplasm / zinc ion binding / ATP binding / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm

ドメイン・相同性:

Tyrosine-protein kinase BTK, SH3 domain / Zinc finger, Btk motif / BTK motif / Zinc finger Btk-type profile. / Bruton's tyrosine kinase Cys-rich motif / PH domain / : / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / SH2 domain superfamily / Src homology 3 domains / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / PH-like domain superfamily / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily

構成要素:

Tyrosine-protein kinase BTK

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: EMBO J / 年: 2003; タイトル: Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering.; 著者: José A Márquez / C I Edvard Smith / Maxim V Petoukhov / Paola Lo Surdo / Pekka T Mattsson / Marika Knekt / Anna Westlund / Klaus Scheffzek / Matti Saraste / Dmitri I Svergun / ; 要旨: Brutons's tyrosine kinase (Btk) is a non-receptor protein tyrosine kinase (nrPTK) essential for the development of B lymphocytes in humans and mice. Like Src and Abl PTKs, Btk contains a conserved cassette formed by SH3, SH2 and protein kinase domains, but differs from them by the presence of an N-terminal PH domain and the Tec homology region. The domain structure of Btk was analysed using X-ray synchrotron radiation scattering in solution. Low resolution shapes of the full-length protein and several deletion mutants determined ab initio from the scattering data indicated a linear arrangement of domains. This arrangement was further confirmed by rigid body modelling using known high resolution structures of individual domains. The final model of Btk displays an extended conformation with no, or little, inter-domain interactions. In agreement with these results, deletion of non-catalytic domains failed to enhance the activity of Btk. Taken together, our results indicate that, contrary to Src and Abl, Btk might not require an assembled conformation for the regulation of its activity.

登録者

• Dmitri Svergun (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #1149: ; タイプ: dummy / ダミー原子の半径: 1.90 A / カイ2乗値: 0.3481 / P-value: 0.790000; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #1150: ; タイプ: mix / ダミー原子の半径: 1.90 A / カイ2乗値: 0.4096 / P-value: 0.003000; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Bruton tyrosine kinase / 試料濃度: 1.00-10.00
• バッファ: 名称: 20 mM HEPES 200 mM NaCl, 2 mM DTT and 1 mM MgCl2 / pH: 7.5

要素 #599

名称: BTK / タイプ: protein / 記述: Tyrosine-protein kinase BTK (R28C mutant) / 分子量: 76.227 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: Q06187
配列:

MAAVILESIF LKRSQQKKKT SPLNFKKCLF LLTVHKLSYY EYDFERGRRG SKKGSIDVEK ITCVETVVPE KNPPPERQIP RRGEESSEME QISIIERFPY PFQVVYDEGP LYVFSPTEEL RKRWIHQLKN VIRYNSDLVQ KYHPCFWIDG QYLCCSQTAK NAMGCQILEN RNGSLKPGSS HRKTKKPLPP TPEEDQILKK PLPPEPAAAP VSTSELKKVV ALYDYMPMNA NDLQLRKGDE YFILEESNLP WWRARDKNGQ EGYIPSNYVT EAEDSIEMYE WYSKHMTRSQ AEQLLKQEGK EGGFIVRDSS KAGKYTVSVF AKSTGDPQGV IRHYVVCSTP QSQYYLAEKH LFSTIPELIN YHQHNSAGLI SRLKYPVSQQ NKNAPSTAGL GYGSWEIDPK DLTFLKELGT GQFGVVKYGK WRGQYDVAIK MIKEGSMSED EFIEEAKVMM NLSHEKLVQL YGVCTKQRPI FIITEYMANG CLLNYLREMR HRFQTQQLLE MCKDVCEAME YLESKQFLHR DLAARNCLVN DQGVVKVSDF GLSRYVLDDE YTSSVGSKFP VRWSPPEVLM YSKFSSKSDI WAFGVLMWEI YSLGKMPYER FTNSETAEHI AQGLRLYRPH LASEKVYTIM YSCWHEKADE RPTFKILLSN ILDVMDEES

実験情報

• ビーム: 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron / 波長: 0.15 Å / スペクトロメータ・検出器間距離: 3 mm
• 検出器: 名称: 1D Gas detector

スキャン

タイトル: Bruton tyrosine kinase / 測定日: 2002年4月2日 / 保管温度: 6 °C / セル温度: 10 °C / 照射時間: 60 sec. / フレーム数: 15 / 単位: 1/A /

	Min	Max
Q	0.0216	0.4575

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 400 /

	Min	Max
Q	0.02164	0.4575
P(R) point	1	400
R	0	200

結果

カーブのタイプ: merged
コメント: The Guinier range is very short subject to experimental conditions

	実験値	Standard	Standard error	Porod
分子量	76 kDa	76 kDa	10	75 kDa
体積	-	-	-	130 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	190	13	183	16
慣性半径, Rg	5.6 nm	0.35	5 nm	4

	Min	Max	Error
D	-	20	2
Guinier point	1	8	-