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- PDB-9v75: Psl polysaccharide related protein structures -

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Basic information

Entry
Database: PDB / ID: 9v75
TitlePsl polysaccharide related protein structures
Components
  • Biofilm formation protein PslD
  • Biofilm formation protein PslE
KeywordsPROTEIN TRANSPORT / complex
Function / homology
Function and homology information


Transferases; Transferring phosphorus-containing groups; Protein-tyrosine kinases / polysaccharide transmembrane transporter activity / porin activity / pore complex / monoatomic ion transport / cell outer membrane / protein tyrosine kinase activity / plasma membrane
Similarity search - Function
Polysaccharide export protein / : / Polysaccharide biosynthesis/export protein / : / : / SLBB domain / Prokaryotic membrane lipoprotein lipid attachment site profile. / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Biofilm formation protein PslE / Biofilm formation protein PslD
Similarity search - Component
Biological speciesPseudomonas aeruginosa (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.89 Å
AuthorsJiao, L. / Yihua, H. / Jiao, L.
Funding support China, 1items
OrganizationGrant numberCountry
Chinese Academy of Sciences China
CitationJournal: Nat Commun / Year: 2026
Title: Structural insights into PSL polysaccharide assembly and export to the cell surface via the Wzx/Wzy-dependent pathway.
Authors: Jiao Liu / Yanlong Han / Lanxin Wang / Luyan Z Ma / Dongchun Ni / Yihua Huang /
Abstract: Bacterial extracellular polysaccharides play a crucial role in mediating pathogen-host interactions and bacterial fitness via biofilm formation. The Wzx/Wzy-dependent pathway is the most prevalent ...Bacterial extracellular polysaccharides play a crucial role in mediating pathogen-host interactions and bacterial fitness via biofilm formation. The Wzx/Wzy-dependent pathway is the most prevalent and conserved strategy for polysaccharide biosynthesis. Psl (polysaccharide synthesis locus) is a key biofilm matrix polysaccharide in Pseudomonas aeruginosa PAO1, and its biosynthesis machinery is predicted to be a Wzx/Wzy-dependent biosynthesis system. Here, using Psl in PAO1 as a model strain, we determine the cryo-EM structures of the PslD-PslE complex. These structures reveal that PslD-PslE complex forms a continuous, protected conduit across the entire cell envelope. Further structural and functional analyses demonstrate that the polymerase PslJ, is likely localized in the membrane lumen formed by the octameric arrangement of PslE's transmembrane helical pairs. We propose a mechanistic model in which Und-PP-linked pentasaccharide units of Psl access PslJ through side portals in the PslE octamer, shielding the polymerization and translocation processes from degradation by PlsG, a periplasm-localized endoglycosidase. The iterative addition of incoming repeat units to the reducing terminus of the growing polysaccharide chain is hypothesized to drive Psl export through the channel, a mechanism that may be conserved across the Wzx/Wzy-dependent polysaccharide biosynthesis pathways.
History
DepositionMay 27, 2025Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Jun 3, 2026Provider: repository / Type: Initial release
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Biofilm formation protein PslE
B: Biofilm formation protein PslE
C: Biofilm formation protein PslD
D: Biofilm formation protein PslE
E: Biofilm formation protein PslD
F: Biofilm formation protein PslE
G: Biofilm formation protein PslD
H: Biofilm formation protein PslE
I: Biofilm formation protein PslD
J: Biofilm formation protein PslE
K: Biofilm formation protein PslD
L: Biofilm formation protein PslE
M: Biofilm formation protein PslD
N: Biofilm formation protein PslE
O: Biofilm formation protein PslD
P: Biofilm formation protein PslD


Theoretical massNumber of molelcules
Total (without water)819,85416
Polymers819,85416
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein
Biofilm formation protein PslE / Tyrosine-protein kinase ptk


Mass: 74543.523 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Pseudomonas aeruginosa (bacteria)
Gene: ptk, CAZ10_28150, GNQ48_26400, PAERUG_P19_London_7_VIM_2_05_10_00389
Production host: Escherichia coli (E. coli)
References: UniProt: A0A073A0N3, Transferases; Transferring phosphorus-containing groups; Protein-tyrosine kinases
#2: Protein
Biofilm formation protein PslD / Polysaccharide biosynthesis/export family protein / Polysaccharide export protein


Mass: 27938.232 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Pseudomonas aeruginosa (bacteria)
Gene: CAZ10_28145, CSB93_4277, GNQ48_26405, IPC1295_16340, L4V69_19100
Production host: Escherichia coli (E. coli) / References: UniProt: A0A140SD11
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: dual complex of Psl transport channel PslD and PslE / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightValue: 828 MDa / Experimental value: NO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: NITROGEN

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 96000 X / Nominal defocus max: 2000 nm / Nominal defocus min: 1200 nm / Cs: 2.7 mm
Specimen holderCryogen: NITROGEN
Image recordingElectron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM software
IDNameCategory
1cryoSPARCparticle selection
13cryoSPARC3D reconstruction
CTF correctionType: NONE
3D reconstructionResolution: 2.89 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 116722 / Symmetry type: POINT

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