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- EMDB-64810: Psl polysaccharide related protein structures -

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Basic information

Entry
Database: EMDB / ID: EMD-64810
TitlePsl polysaccharide related protein structures
Map data
Sample
  • Complex: dual complex of Psl transport channel PslD and PslE
    • Protein or peptide: Biofilm formation protein PslE
    • Protein or peptide: Biofilm formation protein PslD
Keywordscomplex / PROTEIN TRANSPORT
Function / homology
Function and homology information


Transferases; Transferring phosphorus-containing groups; Protein-tyrosine kinases / polysaccharide transmembrane transporter activity / porin activity / pore complex / monoatomic ion transport / cell outer membrane / protein tyrosine kinase activity / plasma membrane
Similarity search - Function
Polysaccharide export protein / : / Polysaccharide biosynthesis/export protein / : / : / SLBB domain / Prokaryotic membrane lipoprotein lipid attachment site profile. / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Biofilm formation protein PslE / Biofilm formation protein PslD
Similarity search - Component
Biological speciesPseudomonas aeruginosa (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.89 Å
AuthorsJiao L / Yihua H
Funding support China, 1 items
OrganizationGrant numberCountry
Chinese Academy of Sciences China
CitationJournal: Nat Commun / Year: 2026
Title: Structural insights into PSL polysaccharide assembly and export to the cell surface via the Wzx/Wzy-dependent pathway.
Authors: Jiao Liu / Yanlong Han / Lanxin Wang / Luyan Z Ma / Dongchun Ni / Yihua Huang /
Abstract: Bacterial extracellular polysaccharides play a crucial role in mediating pathogen-host interactions and bacterial fitness via biofilm formation. The Wzx/Wzy-dependent pathway is the most prevalent ...Bacterial extracellular polysaccharides play a crucial role in mediating pathogen-host interactions and bacterial fitness via biofilm formation. The Wzx/Wzy-dependent pathway is the most prevalent and conserved strategy for polysaccharide biosynthesis. Psl (polysaccharide synthesis locus) is a key biofilm matrix polysaccharide in Pseudomonas aeruginosa PAO1, and its biosynthesis machinery is predicted to be a Wzx/Wzy-dependent biosynthesis system. Here, using Psl in PAO1 as a model strain, we determine the cryo-EM structures of the PslD-PslE complex. These structures reveal that PslD-PslE complex forms a continuous, protected conduit across the entire cell envelope. Further structural and functional analyses demonstrate that the polymerase PslJ, is likely localized in the membrane lumen formed by the octameric arrangement of PslE's transmembrane helical pairs. We propose a mechanistic model in which Und-PP-linked pentasaccharide units of Psl access PslJ through side portals in the PslE octamer, shielding the polymerization and translocation processes from degradation by PlsG, a periplasm-localized endoglycosidase. The iterative addition of incoming repeat units to the reducing terminus of the growing polysaccharide chain is hypothesized to drive Psl export through the channel, a mechanism that may be conserved across the Wzx/Wzy-dependent polysaccharide biosynthesis pathways.
History
DepositionMay 27, 2025-
Header (metadata) releaseJun 3, 2026-
Map releaseJun 3, 2026-
UpdateJul 22, 2026-
Current statusJul 22, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_64810.png

Downloads & links

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Map

FileDownload / File: emd_64810.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
1.03 Å/pix.
x 640 pix.
= 659.2 Å		1.03 Å/pix.
x 640 pix.
= 659.2 Å		1.03 Å/pix.
x 640 pix.
= 659.2 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.03 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.803
Minimum - Maximum-1.9399434 - 4.483198
Average (Standard dev.)-0.0004879072 (±0.093900315)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions640640640
Spacing640640640
CellA=B=C: 659.19995 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_64810_additional_1.map
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Half map: #2

Fileemd_64810_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_64810_half_map_2.map
Projections & Slices
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Sample components

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Entire : dual complex of Psl transport channel PslD and PslE

EntireName: dual complex of Psl transport channel PslD and PslE
Components
  • Complex: dual complex of Psl transport channel PslD and PslE
    • Protein or peptide: Biofilm formation protein PslE
    • Protein or peptide: Biofilm formation protein PslD

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Supramolecule #1: dual complex of Psl transport channel PslD and PslE

SupramoleculeName: dual complex of Psl transport channel PslD and PslE / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 828 MDa

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Macromolecule #1: Biofilm formation protein PslE

MacromoleculeName: Biofilm formation protein PslE / type: protein_or_peptide / ID: 1 / Number of copies: 8 / Enantiomer: LEVO
EC number: Transferases; Transferring phosphorus-containing groups; Protein-tyrosine kinases
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 74.543523 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MIEIRSLRDL LRLLFIYQRE FKLAALSAVV IIVLGAFLLP AKYESNARLL VKPGRDSTLP IEISNRQALV MPSTQRDPIV DEERLLTGR PIVRQVAERY LEVLANRPPP EGLWKRTKFY VKKAIGAVFD GIRVTLETFG VIEETTAVER LAKDLEKKFE V THAAGSTV ...String:
MIEIRSLRDL LRLLFIYQRE FKLAALSAVV IIVLGAFLLP AKYESNARLL VKPGRDSTLP IEISNRQALV MPSTQRDPIV DEERLLTGR PIVRQVAERY LEVLANRPPP EGLWKRTKFY VKKAIGAVFD GIRVTLETFG VIEETTAVER LAKDLEKKFE V THAAGSTV MEISFTWSEP EVAQEVVKAW IEIYMEERTQ ALGRKSLYAF YEAQTADSAA QIKSYKAQIL KHLNEIGASS IE DRLQDLS ERINVLRGER FNSIRLIASS DSALESTRQQ LKGLPREIVT VRQIALNPAQ QDLRRLLNQK RLERADMMRT YTD DAPPVK ALDASIRALE KEVQDEGATV QSSEDRAPNT LTTHLERVLL DETSNNAALR TQLAEQEKQL AELEAQRREA LDIE PTLAR LQRELNATER NYALYVDSLE KSRIDRELDK SQISNISVIE EATYNPGRIF PKTLLMLFLA VPFGLAVGLL VVYLC YLLD QRIHDGGLVE EKFGLPLWTT LPELSNSTAE SSNAFTASIC RLYGLLPYAR IEEKGLTLGL TSARHGEGVT FIIEQL RRL LEENGVRVRI GGEPAQPGEV VLLDAPALLD SCEAFIALRR ADLIALVVEA QKSTVPVVEH ALTILTTAFG KVDGIII NR RRFEVPSKVL QTIARYRSAF

UniProtKB: Biofilm formation protein PslE

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Macromolecule #2: Biofilm formation protein PslD

MacromoleculeName: Biofilm formation protein PslD / type: protein_or_peptide / ID: 2 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 27.938232 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MKRTLLMLAM LALAACNTPA RIPAPDSDTV DSGKRALEEL ARLPPAMERV RVGDTLRIVR DAGEMPTLSA FNVATIYELT LYTVLNDGS IYYPFIGRIQ AAHRTPQEIA NELTTKLAPI YREPRVTVNI NQAPGNTVFV GGAVRNPSAV PIPAANNMEQ A ILGAGGIL ...String:
MKRTLLMLAM LALAACNTPA RIPAPDSDTV DSGKRALEEL ARLPPAMERV RVGDTLRIVR DAGEMPTLSA FNVATIYELT LYTVLNDGS IYYPFIGRIQ AAHRTPQEIA NELTTKLAPI YREPRVTVNI NQAPGNTVFV GGAVRNPSAV PIPAANNMEQ A ILGAGGIL PVGDARRVAL MREDSEGRYH AYFLDFSQLM KIGPEGRKPL AMQRGDIVFV PKSMVGDRIE GVDVYLNQLL PF AKSIGVG VSYTVNNDR

UniProtKB: Biofilm formation protein PslD

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: NITROGEN

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 96000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.89 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 116722
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: PROJECTION MATCHING
FSC plot (resolution estimation)

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