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Yorodumi- PDB-9sob: Structural Model of the Nuclear Pore Complex in Arabidopsis thaliana -
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Open data
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Basic information
| Entry | Database: PDB / ID: 9sob | |||||||||||||||
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| Title | Structural Model of the Nuclear Pore Complex in Arabidopsis thaliana | |||||||||||||||
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Keywords | NUCLEAR PROTEIN / Nuclear Pore Complex / Nuclear Transport / Nuclear-cytoplasmic transport | |||||||||||||||
| Function / homology | Function and homology informationnegative regulation of auxin mediated signaling pathway / regulation of timing of transition from vegetative to reproductive phase / long-day photoperiodism, flowering / nuclear pore transmembrane ring / zygote asymmetric cell division / induced systemic resistance / vegetative to reproductive phase transition of meristem / systemic acquired resistance, salicylic acid mediated signaling pathway / systemic acquired resistance / innate immune response-activating signaling pathway ...negative regulation of auxin mediated signaling pathway / regulation of timing of transition from vegetative to reproductive phase / long-day photoperiodism, flowering / nuclear pore transmembrane ring / zygote asymmetric cell division / induced systemic resistance / vegetative to reproductive phase transition of meristem / systemic acquired resistance, salicylic acid mediated signaling pathway / systemic acquired resistance / innate immune response-activating signaling pathway / nuclear pore inner ring / developmental process / nuclear pore organization / response to auxin / embryo development ending in seed dormancy / response to abscisic acid / plasmodesma / structural constituent of nuclear pore / RNA export from nucleus / nucleocytoplasmic transport / poly(A)+ mRNA export from nucleus / porin activity / ribosomal large subunit export from nucleus / mRNA transport / nuclear pore / mRNA export from nucleus / ribosomal small subunit export from nucleus / vesicle-mediated transport / nuclear periphery / protein export from nucleus / response to cold / response to bacterium / circadian regulation of gene expression / defense response / RING-type E3 ubiquitin transferase / protein import into nucleus / ubiquitin-protein transferase activity / nuclear envelope / protein transport / nuclear membrane / protein-macromolecule adaptor activity / nucleic acid binding / protein ubiquitination / innate immune response / negative regulation of DNA-templated transcription / nucleolus / structural molecule activity / endoplasmic reticulum / Golgi apparatus / mitochondrion / zinc ion binding / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | |||||||||||||||
| Biological species | ![]() | |||||||||||||||
| Method | ELECTRON MICROSCOPY / subtomogram averaging / cryo EM / Resolution: 35 Å | |||||||||||||||
Authors | Obarska-Kosinska, A. / Sanchez Carrillo, I.B. / Hoffmann, P.C. / Fourcassie, V. / Beck, M. / Germain, H. | |||||||||||||||
| Funding support | Germany, European Union, Canada, 4items
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Citation | Journal: Nat.Plants / Year: 2025Title: In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana Authors: Sanchez Carrillo, I.B. / Hoffmann, P.C. / Obarska-Kosinska, A. / Fourcassie, V. / Beck, M. / Germain, H. | |||||||||||||||
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9sob.cif.gz | 8.5 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9sob.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9sob.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Summary document | 9sob_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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| Full document | 9sob_full_validation.pdf.gz | 4.2 MB | Display | |
| Data in XML | 9sob_validation.xml.gz | 1.3 MB | Display | |
| Data in CIF | 9sob_validation.cif.gz | 1.8 MB | Display | |
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/so/9sob ftp://data.pdbj.org/pub/pdb/validation_reports/so/9sob | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 54657 M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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| 1 | x 15![]()
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Components
-Nuclear pore complex protein ... , 17 types, 51 molecules RR8R16MM8M16PP8P16QQ8Q16LL8L16KK8K16C16C24CC8C32A24A40A16A32AA48V...
| #1: Protein | Mass: 168456.297 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Protein | Mass: 80453.953 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #5: Protein | Mass: 80946.992 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #7: Protein | Mass: 39388.680 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #8: Protein | Mass: 111106.227 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #9: Protein | Mass: 81469.633 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #10: Protein | Mass: 206947.188 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) ![]() #11: Protein | Mass: 96591.953 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #12: Protein | Mass: 85783.711 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #13: Protein | | Mass: 22728.789 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #14: Protein | | Mass: 89832.617 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #15: Protein | | Mass: 21492.982 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #16: Protein | | Mass: 7520.575 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #17: Protein/peptide | Mass: 3045.227 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #21: Protein | Mass: 28956.146 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #23: Protein | Mass: 22892.584 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #24: Protein | Mass: 160181.938 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Protein , 7 types, 19 molecules NN8N16TT8T16OO8O16BB8448EE8II8I24I16
| #3: Protein | Mass: 32674.375 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #4: Protein | Mass: 78435.930 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: Q84JU6, RING-type E3 ubiquitin transferase #6: Protein | Mass: 35729.016 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #18: Protein | Mass: 219705.188 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #19: Protein | Mass: 48532.457 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #20: Protein | Mass: 57475.391 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #22: Protein | Mass: 27626.533 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Details
| Has protein modification | N |
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-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: CELL / 3D reconstruction method: subtomogram averaging |
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Sample preparation
| Component | Name: Nuclear Pore Complex / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: NATURAL |
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| Source (natural) | Organism: ![]() |
| Buffer solution | pH: 5.7 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER |
| Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 5000 nm / Nominal defocus min: 2500 nm |
| Image recording | Electron dose: 2.2 e/Å2 / Avg electron dose per subtomogram: 130 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
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| CTF correction | Type: PHASE FLIPPING ONLY | |||||||||
| Symmetry | Point symmetry: C8 (8 fold cyclic) | |||||||||
| 3D reconstruction | Resolution: 35 Å / Resolution method: OTHER / Num. of particles: 75 / Symmetry type: POINT | |||||||||
| EM volume selection | Num. of tomograms: 37 / Num. of volumes extracted: 79 |
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Germany, European Union,
Canada, 4items
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FIELD EMISSION GUN