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- PDB-9sob: Structural Model of the Nuclear Pore Complex in Arabidopsis thaliana -

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Entry
Database: PDB / ID: 9sob
TitleStructural Model of the Nuclear Pore Complex in Arabidopsis thaliana
Components
  • (Nuclear pore complex protein ...) x 17
  • Aladin
  • E3 ubiquitin-protein ligase HOS1
  • NUP58
  • Nucleoporin (DUF3414)
  • Nucleoporin protein Ndc1-Nup protein
  • Protein SEH1
  • Protein transport protein SEC13 homolog B
KeywordsNUCLEAR PROTEIN / Nuclear Pore Complex / Nuclear Transport / Nuclear-cytoplasmic transport
Function / homology
Function and homology information


negative regulation of auxin mediated signaling pathway / regulation of timing of transition from vegetative to reproductive phase / long-day photoperiodism, flowering / nuclear pore transmembrane ring / zygote asymmetric cell division / induced systemic resistance / vegetative to reproductive phase transition of meristem / systemic acquired resistance / systemic acquired resistance, salicylic acid mediated signaling pathway / innate immune response-activating signaling pathway ...negative regulation of auxin mediated signaling pathway / regulation of timing of transition from vegetative to reproductive phase / long-day photoperiodism, flowering / nuclear pore transmembrane ring / zygote asymmetric cell division / induced systemic resistance / vegetative to reproductive phase transition of meristem / systemic acquired resistance / systemic acquired resistance, salicylic acid mediated signaling pathway / innate immune response-activating signaling pathway / nuclear pore inner ring / developmental process / nuclear pore organization / response to auxin / embryo development ending in seed dormancy / response to abscisic acid / plasmodesma / structural constituent of nuclear pore / RNA export from nucleus / nucleocytoplasmic transport / poly(A)+ mRNA export from nucleus / porin activity / ribosomal large subunit export from nucleus / mRNA transport / nuclear pore / mRNA export from nucleus / vesicle-mediated transport / ribosomal small subunit export from nucleus / nuclear periphery / protein export from nucleus / response to cold / response to bacterium / circadian regulation of gene expression / defense response / RING-type E3 ubiquitin transferase / protein import into nucleus / ubiquitin-protein transferase activity / nuclear envelope / protein transport / nuclear membrane / protein-macromolecule adaptor activity / nucleic acid binding / protein ubiquitination / innate immune response / negative regulation of DNA-templated transcription / nucleolus / structural molecule activity / endoplasmic reticulum / Golgi apparatus / mitochondrion / zinc ion binding / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Nuclear pore complex protein NUP160 / Nuclear pore complex protein NUP214 / E3 ubiquitin-protein ligase HOS1 / NUP160, helical domain / Nucleoporin protein Ndc1-Nup / Aladin seven-bladed propeller / Nucleoporin, NUP53 / Aladin / ELYS-like domain / Nuclear pore complex assembly ...Nuclear pore complex protein NUP160 / Nuclear pore complex protein NUP214 / E3 ubiquitin-protein ligase HOS1 / NUP160, helical domain / Nucleoporin protein Ndc1-Nup / Aladin seven-bladed propeller / Nucleoporin, NUP53 / Aladin / ELYS-like domain / Nuclear pore complex assembly / Nucleoporin Nup88 / Nuclear pore component / : / : / Nup160 C-terminal TPR / NUP160/120 middle TPR / RNA-recognition motif (RRM) Nup35-type domain / Nup53/35/40-type RNA recognition motif / RNA-recognition motif (RRM) Nup35-type domain profile. / Nucleoporin NUP88/NUP82 / Nucleoporin Nup188 / Nucleoporin, Nup155-like, C-terminal, subdomain 3 / Nucleoporin Nup120/160, beta-propeller domain / Nucleoporin Nup54/Nup57/Nup44 / Nucleoporin Nup54, alpha-helical domain / Nucleoporin complex subunit 54 / Nucleoporin, NSP1-like, C-terminal / Nucleoporin NSP1/NUP62 / Nsp1-like C-terminal region / Nucleoporin Nup85-like / Nucleoporin Nup120/160 / Nup85 Nucleoporin / Nuclear pore protein 84/107 / Nuclear pore protein 84 / 107 / Nuclear pore complex protein Nup133-like / Nucleoporin, Nup155-like, C-terminal, subdomain 1 / Nucleoporin, Nup155-like, C-terminal, subdomain 2 / Nucleoporin, Nup155-like / Nucleoporin Nup186/Nup192/Nup205 / Nuclear pore complex scaffold, nucleoporins 186/192/205 / Nucleoporin, Nup133/Nup155-like, C-terminal / Non-repetitive/WGA-negative nucleoporin C-terminal / Nucleoporin interacting component Nup93/Nic96 / Nup93/Nic96 / Nucleoporin, Nup133/Nup155-like, N-terminal / Nup133 N terminal like / Nuclear pore complex protein NUP96, C-terminal domain / Nuclear protein 96 / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain superfamily / Sec13/Seh1 family / Nucleoporin peptidase S59-like / Nup98-96 autopeptidase S59 / NUP C-terminal domain profile. / RNA-binding domain superfamily / WD domain, G-beta repeat / Zinc finger, RING/FYVE/PHD-type / G-protein beta WD-40 repeat / Nucleotide-binding alpha-beta plait domain superfamily / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Nuclear pore complex protein NUP155 / Nuclear pore complex protein NUP214 / Nuclear pore complex protein NUP133 / Nucleoporin (DUF3414) / Nuclear pore complex protein NUP205 / Nuclear pore complex protein NUP35 / Nuclear pore complex protein NUP93A / Protein transport protein SEC13 homolog B / Nuclear pore complex protein NUP43 / E3 ubiquitin-protein ligase HOS1 ...Nuclear pore complex protein NUP155 / Nuclear pore complex protein NUP214 / Nuclear pore complex protein NUP133 / Nucleoporin (DUF3414) / Nuclear pore complex protein NUP205 / Nuclear pore complex protein NUP35 / Nuclear pore complex protein NUP93A / Protein transport protein SEC13 homolog B / Nuclear pore complex protein NUP43 / E3 ubiquitin-protein ligase HOS1 / Aladin / Nuclear pore complex protein NUP54 / Nuclear pore complex protein NUP107 / Nuclear pore complex protein NUP62 / Nucleoporin protein Ndc1-Nup protein / Nuclear pore complex protein NUP96 / Nuclear pore complex protein NUP85 / Protein SEH1 / Nuclear pore complex protein NUP160 / Nuclear pore complex protein NUP88
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
MethodELECTRON MICROSCOPY / subtomogram averaging / cryo EM / Resolution: 35 Å
AuthorsObarska-Kosinska, A. / Sanchez Carrillo, I.B. / Hoffmann, P.C. / Fourcassie, V. / Beck, M. / Germain, H.
Funding support Germany, European Union, Canada, 4items
OrganizationGrant numberCountry
Max Planck Society Germany
European Research Council (ERC)NPCvalve 101054823European Union
Natural Sciences and Engineering Research Council (NSERC, Canada)RG2020-04002 Canada
European Molecular Biology Organization (EMBO)ALTF 33-2021European Union
CitationJournal: Nat Plants / Year: 2025
Title: In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana.
Authors: Ingrid Berenice Sanchez Carrillo / Patrick C Hoffmann / Agnieszka Obarska-Kosinska / Victor Fourcassié / Martin Beck / Hugo Germain /
Abstract: The nucleus is enclosed by the nuclear envelope, which contains nuclear pore complexes (NPCs). While NPCs have been well studied in vertebrates, yeast and algae, in situ structural data for higher ...The nucleus is enclosed by the nuclear envelope, which contains nuclear pore complexes (NPCs). While NPCs have been well studied in vertebrates, yeast and algae, in situ structural data for higher plants is lacking. Here we show that individual nucleoporins of Arabidopsis thaliana and humans exhibit high structural similarity. We report an in situ NPC structure of higher plants, derived from A. thaliana root protoplasts using cryo-electron tomography, subtomogram averaging and homology-based integrative modelling. We present the AtNPC model based on predictions of A. thaliana nucleoporins (NUPs), supported by mass spectrometry. Here the AtNPC scaffold contains one Y-complex ring at the cytosolic and two at the nuclear ring. The AtNPC contains prominent NUP155 connector elements that are conserved in human NPCs but not in Chlamydomonas reinhardtii NPCs. Our model suggests that the ELYS homologue HOS1 plays an important role in the head-to-tail connection of Y-complexes in AtNPCs.
History
DepositionSep 12, 2025Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 5, 2025Provider: repository / Type: Initial release
Revision 1.1Nov 12, 2025Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Dec 3, 2025Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
R: Nuclear pore complex protein NUP160
M: Nuclear pore complex protein NUP96
N: Protein transport protein SEC13 homolog B
R8: Nuclear pore complex protein NUP160
M8: Nuclear pore complex protein NUP96
N8: Protein transport protein SEC13 homolog B
R16: Nuclear pore complex protein NUP160
M16: Nuclear pore complex protein NUP96
N16: Protein transport protein SEC13 homolog B
T: E3 ubiquitin-protein ligase HOS1
T8: E3 ubiquitin-protein ligase HOS1
T16: E3 ubiquitin-protein ligase HOS1
P: Nuclear pore complex protein NUP85
O: Protein SEH1
Q: Nuclear pore complex protein NUP43
P8: Nuclear pore complex protein NUP85
O8: Protein SEH1
Q8: Nuclear pore complex protein NUP43
P16: Nuclear pore complex protein NUP85
O16: Protein SEH1
Q16: Nuclear pore complex protein NUP43
L: Nuclear pore complex protein NUP107
L8: Nuclear pore complex protein NUP107
L16: Nuclear pore complex protein NUP107
K: Nuclear pore complex protein NUP133
K8: Nuclear pore complex protein NUP133
K16: Nuclear pore complex protein NUP133
C16: Nuclear pore complex protein NUP205
A24: Nuclear pore complex protein NUP93A
C24: Nuclear pore complex protein NUP205
A40: Nuclear pore complex protein NUP93A
A: Nuclear pore complex protein NUP93A
V: Nuclear pore complex protein NUP214
W: Nuclear pore complex protein NUP88
J: Nuclear pore complex protein NUP62
C: Nuclear pore complex protein NUP205
C8: Nuclear pore complex protein NUP205
A8: Nuclear pore complex protein NUP93A
A16: Nuclear pore complex protein NUP93A
F8: Nuclear pore complex protein NUP35
F: Nuclear pore complex protein NUP35
A32: Nuclear pore complex protein NUP93A
F24: Nuclear pore complex protein NUP35
F16: Nuclear pore complex protein NUP35
B: Nucleoporin (DUF3414)
B8: Nucleoporin (DUF3414)
4: Aladin
E: Nucleoporin protein Ndc1-Nup protein
48: Aladin
E8: Nucleoporin protein Ndc1-Nup protein
H: Nuclear pore complex protein NUP54
I: NUP58
J32: Nuclear pore complex protein NUP62
H8: Nuclear pore complex protein NUP54
I8: NUP58
J8: Nuclear pore complex protein NUP62
H24: Nuclear pore complex protein NUP54
I24: NUP58
J24: Nuclear pore complex protein NUP62
H16: Nuclear pore complex protein NUP54
I16: NUP58
J16: Nuclear pore complex protein NUP62
D: Nuclear pore complex protein NUP155
D8: Nuclear pore complex protein NUP155
D16: Nuclear pore complex protein NUP155
D24: Nuclear pore complex protein NUP155
D32: Nuclear pore complex protein NUP155
D40: Nuclear pore complex protein NUP155
C32: Nuclear pore complex protein NUP205
A48: Nuclear pore complex protein NUP93A


Theoretical massNumber of molelcules
Total (without water)5,802,82970
Polymers5,802,82970
Non-polymers00
Water00
1
R: Nuclear pore complex protein NUP160
M: Nuclear pore complex protein NUP96
N: Protein transport protein SEC13 homolog B
R8: Nuclear pore complex protein NUP160
M8: Nuclear pore complex protein NUP96
N8: Protein transport protein SEC13 homolog B
R16: Nuclear pore complex protein NUP160
M16: Nuclear pore complex protein NUP96
N16: Protein transport protein SEC13 homolog B
T: E3 ubiquitin-protein ligase HOS1
T8: E3 ubiquitin-protein ligase HOS1
T16: E3 ubiquitin-protein ligase HOS1
P: Nuclear pore complex protein NUP85
O: Protein SEH1
Q: Nuclear pore complex protein NUP43
P8: Nuclear pore complex protein NUP85
O8: Protein SEH1
Q8: Nuclear pore complex protein NUP43
P16: Nuclear pore complex protein NUP85
O16: Protein SEH1
Q16: Nuclear pore complex protein NUP43
L: Nuclear pore complex protein NUP107
L8: Nuclear pore complex protein NUP107
L16: Nuclear pore complex protein NUP107
K: Nuclear pore complex protein NUP133
K8: Nuclear pore complex protein NUP133
K16: Nuclear pore complex protein NUP133
C16: Nuclear pore complex protein NUP205
A24: Nuclear pore complex protein NUP93A
C24: Nuclear pore complex protein NUP205
A40: Nuclear pore complex protein NUP93A
A: Nuclear pore complex protein NUP93A
V: Nuclear pore complex protein NUP214
W: Nuclear pore complex protein NUP88
J: Nuclear pore complex protein NUP62
C: Nuclear pore complex protein NUP205
C8: Nuclear pore complex protein NUP205
A8: Nuclear pore complex protein NUP93A
A16: Nuclear pore complex protein NUP93A
F8: Nuclear pore complex protein NUP35
F: Nuclear pore complex protein NUP35
A32: Nuclear pore complex protein NUP93A
F24: Nuclear pore complex protein NUP35
F16: Nuclear pore complex protein NUP35
B: Nucleoporin (DUF3414)
B8: Nucleoporin (DUF3414)
4: Aladin
E: Nucleoporin protein Ndc1-Nup protein
48: Aladin
E8: Nucleoporin protein Ndc1-Nup protein
H: Nuclear pore complex protein NUP54
I: NUP58
J32: Nuclear pore complex protein NUP62
H8: Nuclear pore complex protein NUP54
I8: NUP58
J8: Nuclear pore complex protein NUP62
H24: Nuclear pore complex protein NUP54
I24: NUP58
J24: Nuclear pore complex protein NUP62
H16: Nuclear pore complex protein NUP54
I16: NUP58
J16: Nuclear pore complex protein NUP62
D: Nuclear pore complex protein NUP155
D8: Nuclear pore complex protein NUP155
D16: Nuclear pore complex protein NUP155
D24: Nuclear pore complex protein NUP155
D32: Nuclear pore complex protein NUP155
D40: Nuclear pore complex protein NUP155
C32: Nuclear pore complex protein NUP205
A48: Nuclear pore complex protein NUP93A
x 15


Theoretical massNumber of molelcules
Total (without water)87,042,4371050
Polymers87,042,4371050
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
point symmetry operation14

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Components

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Nuclear pore complex protein ... , 17 types, 51 molecules RR8R16MM8M16PP8P16QQ8Q16LL8L16KK8K16C16C24CC8C32A24A40A16A32AA48V...

#1: Protein Nuclear pore complex protein NUP160 / AtNUP160 / Nucleoporin 160 / Protein SUPPRESSOR OF AUXIN RESISTANCE 1


Mass: 168456.297 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q9C811
#2: Protein Nuclear pore complex protein NUP96 / AtNUP96 / Nucleoporin 96 / Nucleoporin PRECOCIOUS / Nucleoporin PRECOZ / Protein MODIFIER OF SNC1 3 ...AtNUP96 / Nucleoporin 96 / Nucleoporin PRECOCIOUS / Nucleoporin PRECOZ / Protein MODIFIER OF SNC1 3 / Protein SUPPRESSOR OF AUXIN RESISTANCE 3


Mass: 80453.953 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q8LLD0
#5: Protein Nuclear pore complex protein NUP85 / Nuclear pore complex protein NUP75 / Nucleoporin 75 / Nucleoporin 85


Mass: 80946.992 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q8RXH2
#7: Protein Nuclear pore complex protein NUP43 / Nucleoporin 43


Mass: 39388.680 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q24JJ9
#8: Protein Nuclear pore complex protein NUP107 / Nucleoporin 107


Mass: 111106.227 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q8L748
#9: Protein Nuclear pore complex protein NUP133 / Nucleoporin 133 / Nucleoporin Nup155-like


Mass: 81469.633 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: F4IGA5
#10: Protein
Nuclear pore complex protein NUP205 / Nucleoporin 205 / Protein EMBRYO DEFECTIVE 3142


Mass: 206947.188 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: F4KBW6
#11: Protein
Nuclear pore complex protein NUP93A / Nucleoporin 93A


Mass: 96591.953 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: O22224
#12: Protein Nuclear pore complex protein NUP93A / Nucleoporin 93A


Mass: 85783.711 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: O22224
#13: Protein Nuclear pore complex protein NUP214 / Nucleoporin 214 / Protein EMBRYO DEFECTIVE 1011 / Protein LNO1 / Protein LONO1


Mass: 22728.789 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: F4I1T7
#14: Protein Nuclear pore complex protein NUP88 / Nucleoporin 88 / Protein EMBRYO DEFECTIVE 2789 / Protein MODIFIER OF SNC1 / 7


Mass: 89832.617 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q9FFK6
#15: Protein Nuclear pore complex protein NUP62 / Nucleoporin 62 / NP62 / Protein EMBRYO DEFECTIVE 2766


Mass: 21492.982 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q8L7F7
#16: Protein Nuclear pore complex protein NUP93A / Nucleoporin 93A


Mass: 7520.575 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: O22224
#17: Protein/peptide
Nuclear pore complex protein NUP35 / Nucleoporin 35


Mass: 3045.227 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: O04326
#21: Protein
Nuclear pore complex protein NUP54 / Nucleoporin 54


Mass: 28956.146 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q8GYF7
#23: Protein
Nuclear pore complex protein NUP62 / Nucleoporin 62 / NP62 / Protein EMBRYO DEFECTIVE 2766


Mass: 22892.584 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q8L7F7
#24: Protein
Nuclear pore complex protein NUP155 / Nucleoporin 155


Mass: 160181.938 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: F4HXV6

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Protein , 7 types, 19 molecules NN8N16TT8T16OO8O16BB8448EE8II8I24I16

#3: Protein Protein transport protein SEC13 homolog B / SEC13-like protein B


Mass: 32674.375 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: O64740
#4: Protein E3 ubiquitin-protein ligase HOS1 / Protein HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 / RING finger protein HOS1 / RING-type E3 ...Protein HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 / RING finger protein HOS1 / RING-type E3 ubiquitin transferase HOS1


Mass: 78435.930 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress)
References: UniProt: Q84JU6, RING-type E3 ubiquitin transferase
#6: Protein Protein SEH1


Mass: 35729.016 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q93VR9
#18: Protein Nucleoporin (DUF3414)


Mass: 219705.188 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: F4JUG3
#19: Protein Aladin


Mass: 48532.457 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q8GWR1
#20: Protein Nucleoporin protein Ndc1-Nup protein


Mass: 57475.391 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress) / References: UniProt: Q8LAF4
#22: Protein
NUP58


Mass: 27626.533 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Arabidopsis thaliana (thale cress)

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Details

Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: CELL / 3D reconstruction method: subtomogram averaging

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Sample preparation

ComponentName: Nuclear Pore Complex / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: NATURAL
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Buffer solutionpH: 5.7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 5000 nm / Nominal defocus min: 2500 nm
Image recordingElectron dose: 2.2 e/Å2 / Avg electron dose per subtomogram: 130 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

EM software
IDNameCategory
1novaSTAvolume selection
13novaSTA3D reconstruction
CTF correctionType: PHASE FLIPPING ONLY
SymmetryPoint symmetry: C8 (8 fold cyclic)
3D reconstructionResolution: 35 Å / Resolution method: OTHER / Num. of particles: 75 / Symmetry type: POINT
EM volume selectionNum. of tomograms: 37 / Num. of volumes extracted: 79

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